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Smoking cessation affects human platelet activation induced by collagen

It is firmly established that smoking is a risk factor of cardiovascular disease, stroke and peripheral vascular disease. Although smoking alters the hemostatic process, the influence of smoking on human platelet activation remains controversial. For patients undergoing surgery, cessation of smoking...

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Autores principales: Kito, Yuko, Iida, Mami, Tanabe, Kumiko, Onuma, Takashi, Tsujimoto, Masanori, Nagase, Kiyoshi, Tokuda, Haruhiko, Iwama, Toru, Kozawa, Osamu, Iida, Hiroki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6781815/
https://www.ncbi.nlm.nih.gov/pubmed/31616510
http://dx.doi.org/10.3892/etm.2019.8025
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author Kito, Yuko
Iida, Mami
Tanabe, Kumiko
Onuma, Takashi
Tsujimoto, Masanori
Nagase, Kiyoshi
Tokuda, Haruhiko
Iwama, Toru
Kozawa, Osamu
Iida, Hiroki
author_facet Kito, Yuko
Iida, Mami
Tanabe, Kumiko
Onuma, Takashi
Tsujimoto, Masanori
Nagase, Kiyoshi
Tokuda, Haruhiko
Iwama, Toru
Kozawa, Osamu
Iida, Hiroki
author_sort Kito, Yuko
collection PubMed
description It is firmly established that smoking is a risk factor of cardiovascular disease, stroke and peripheral vascular disease. Although smoking alters the hemostatic process, the influence of smoking on human platelet activation remains controversial. For patients undergoing surgery, cessation of smoking prior to the procedure is recommended as it increases the risk of postoperative morbidity or mortality. The presented study investigated the effects of smoking cessation on human platelet activation induced via collagen (n=19 patients). Blood samples were taken on four occasions: Before smoking cessation, and at 4, 8 and 12 weeks after smoking cessation. Platelet aggregation using citrated platelet-rich plasma (PRP) was monitored using a PA-200 aggregometer, which determined the size of platelet aggregates using laser scattering methods. A low dose of collagen (1 µg/ml) accelerated platelet aggregation at 4 or 8 weeks after smoking cessation when compared with results before cessation. After 12 weeks, levels of platelet aggregation induced by collagen were almost equal to those recorded prior to smoking cessation. The secretion levels of collagen-induced platelet-derived growth factor (PDGF)-AB at 4 or 8 weeks after smoking cessation were significantly higher than those before smoking was stopped. Furthermore, smoking cessation markedly strengthened the collagen-induced phosphorylation of p38 mitogen-activated protein (MAP) kinase after 4 weeks. The results of the current study indicated that smoking cessation causes temporary short-term human platelet hyper-activation. The further suggest that the incidence of complications due to human platelet hyper-reactivity may be lowered by considering the period of smoking abstinence.
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spelling pubmed-67818152019-10-15 Smoking cessation affects human platelet activation induced by collagen Kito, Yuko Iida, Mami Tanabe, Kumiko Onuma, Takashi Tsujimoto, Masanori Nagase, Kiyoshi Tokuda, Haruhiko Iwama, Toru Kozawa, Osamu Iida, Hiroki Exp Ther Med Articles It is firmly established that smoking is a risk factor of cardiovascular disease, stroke and peripheral vascular disease. Although smoking alters the hemostatic process, the influence of smoking on human platelet activation remains controversial. For patients undergoing surgery, cessation of smoking prior to the procedure is recommended as it increases the risk of postoperative morbidity or mortality. The presented study investigated the effects of smoking cessation on human platelet activation induced via collagen (n=19 patients). Blood samples were taken on four occasions: Before smoking cessation, and at 4, 8 and 12 weeks after smoking cessation. Platelet aggregation using citrated platelet-rich plasma (PRP) was monitored using a PA-200 aggregometer, which determined the size of platelet aggregates using laser scattering methods. A low dose of collagen (1 µg/ml) accelerated platelet aggregation at 4 or 8 weeks after smoking cessation when compared with results before cessation. After 12 weeks, levels of platelet aggregation induced by collagen were almost equal to those recorded prior to smoking cessation. The secretion levels of collagen-induced platelet-derived growth factor (PDGF)-AB at 4 or 8 weeks after smoking cessation were significantly higher than those before smoking was stopped. Furthermore, smoking cessation markedly strengthened the collagen-induced phosphorylation of p38 mitogen-activated protein (MAP) kinase after 4 weeks. The results of the current study indicated that smoking cessation causes temporary short-term human platelet hyper-activation. The further suggest that the incidence of complications due to human platelet hyper-reactivity may be lowered by considering the period of smoking abstinence. D.A. Spandidos 2019-11 2019-09-18 /pmc/articles/PMC6781815/ /pubmed/31616510 http://dx.doi.org/10.3892/etm.2019.8025 Text en Copyright: © Kito et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Kito, Yuko
Iida, Mami
Tanabe, Kumiko
Onuma, Takashi
Tsujimoto, Masanori
Nagase, Kiyoshi
Tokuda, Haruhiko
Iwama, Toru
Kozawa, Osamu
Iida, Hiroki
Smoking cessation affects human platelet activation induced by collagen
title Smoking cessation affects human platelet activation induced by collagen
title_full Smoking cessation affects human platelet activation induced by collagen
title_fullStr Smoking cessation affects human platelet activation induced by collagen
title_full_unstemmed Smoking cessation affects human platelet activation induced by collagen
title_short Smoking cessation affects human platelet activation induced by collagen
title_sort smoking cessation affects human platelet activation induced by collagen
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6781815/
https://www.ncbi.nlm.nih.gov/pubmed/31616510
http://dx.doi.org/10.3892/etm.2019.8025
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