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Correlative vibrational spectroscopy and 2D X-ray diffraction to probe the mineralization of bone in phosphate-deficient mice

Bone crystallite chemistry and structure change during bone maturation. However, these properties of bone can also be affected by limited uptake of the chemical constituents of the mineral by the animal. This makes probing the effect of bone-mineralization-related diseases a complicated task. Here i...

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Autores principales: King, Helen E., Tommasini, Steven M., Rodriguez-Navarro, Alejandro B., Mercado, Brandon Q., Skinner, H. Catherine W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6782074/
https://www.ncbi.nlm.nih.gov/pubmed/31636517
http://dx.doi.org/10.1107/S1600576719009361
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author King, Helen E.
Tommasini, Steven M.
Rodriguez-Navarro, Alejandro B.
Mercado, Brandon Q.
Skinner, H. Catherine W.
author_facet King, Helen E.
Tommasini, Steven M.
Rodriguez-Navarro, Alejandro B.
Mercado, Brandon Q.
Skinner, H. Catherine W.
author_sort King, Helen E.
collection PubMed
description Bone crystallite chemistry and structure change during bone maturation. However, these properties of bone can also be affected by limited uptake of the chemical constituents of the mineral by the animal. This makes probing the effect of bone-mineralization-related diseases a complicated task. Here it is shown that the combination of vibrational spectroscopy with two-dimensional X-ray diffraction can provide unparalleled information on the changes in bone chemistry and structure associated with different bone pathologies (phosphate deficiency) and/or health conditions (pregnancy, lactation). Using a synergistic analytical approach, it was possible to trace the effect that changes in the remodelling regime have on the bone mineral chemistry and structure in normal and mineral-deficient (hypophosphatemic) mice. The results indicate that hypophosphatemic mice have increased bone remodelling, increased carbonate content and decreased crystallinity of the bone mineral, as well as increased misalignment of crystallites within the bone tissue. Pregnant and lactating mice that are normal and hypophosphatemic showed changes in the chemistry and misalignment of the apatite crystals that can be related to changes in remodelling rates associated with different calcium demand during pregnancy and lactation.
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spelling pubmed-67820742019-10-21 Correlative vibrational spectroscopy and 2D X-ray diffraction to probe the mineralization of bone in phosphate-deficient mice King, Helen E. Tommasini, Steven M. Rodriguez-Navarro, Alejandro B. Mercado, Brandon Q. Skinner, H. Catherine W. J Appl Crystallogr Research Papers Bone crystallite chemistry and structure change during bone maturation. However, these properties of bone can also be affected by limited uptake of the chemical constituents of the mineral by the animal. This makes probing the effect of bone-mineralization-related diseases a complicated task. Here it is shown that the combination of vibrational spectroscopy with two-dimensional X-ray diffraction can provide unparalleled information on the changes in bone chemistry and structure associated with different bone pathologies (phosphate deficiency) and/or health conditions (pregnancy, lactation). Using a synergistic analytical approach, it was possible to trace the effect that changes in the remodelling regime have on the bone mineral chemistry and structure in normal and mineral-deficient (hypophosphatemic) mice. The results indicate that hypophosphatemic mice have increased bone remodelling, increased carbonate content and decreased crystallinity of the bone mineral, as well as increased misalignment of crystallites within the bone tissue. Pregnant and lactating mice that are normal and hypophosphatemic showed changes in the chemistry and misalignment of the apatite crystals that can be related to changes in remodelling rates associated with different calcium demand during pregnancy and lactation. International Union of Crystallography 2019-08-23 /pmc/articles/PMC6782074/ /pubmed/31636517 http://dx.doi.org/10.1107/S1600576719009361 Text en © Helen E. King et al. 2019 http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/4.0/
spellingShingle Research Papers
King, Helen E.
Tommasini, Steven M.
Rodriguez-Navarro, Alejandro B.
Mercado, Brandon Q.
Skinner, H. Catherine W.
Correlative vibrational spectroscopy and 2D X-ray diffraction to probe the mineralization of bone in phosphate-deficient mice
title Correlative vibrational spectroscopy and 2D X-ray diffraction to probe the mineralization of bone in phosphate-deficient mice
title_full Correlative vibrational spectroscopy and 2D X-ray diffraction to probe the mineralization of bone in phosphate-deficient mice
title_fullStr Correlative vibrational spectroscopy and 2D X-ray diffraction to probe the mineralization of bone in phosphate-deficient mice
title_full_unstemmed Correlative vibrational spectroscopy and 2D X-ray diffraction to probe the mineralization of bone in phosphate-deficient mice
title_short Correlative vibrational spectroscopy and 2D X-ray diffraction to probe the mineralization of bone in phosphate-deficient mice
title_sort correlative vibrational spectroscopy and 2d x-ray diffraction to probe the mineralization of bone in phosphate-deficient mice
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6782074/
https://www.ncbi.nlm.nih.gov/pubmed/31636517
http://dx.doi.org/10.1107/S1600576719009361
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