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Replicating Arabidopsis Model Leaf Surfaces for Phyllosphere Microbiology
Artificial surfaces are commonly used in place of leaves in phyllosphere microbiology to study microbial behaviour on plant leaf surfaces. These surfaces enable a reductionist approach to be undertaken, to enable individual environmental factors influencing microorganisms to be studied. Commonly use...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6783459/ https://www.ncbi.nlm.nih.gov/pubmed/31595008 http://dx.doi.org/10.1038/s41598-019-50983-7 |
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author | Soffe, Rebecca Bernach, Michal Remus-Emsermann, Mitja N. P. Nock, Volker |
author_facet | Soffe, Rebecca Bernach, Michal Remus-Emsermann, Mitja N. P. Nock, Volker |
author_sort | Soffe, Rebecca |
collection | PubMed |
description | Artificial surfaces are commonly used in place of leaves in phyllosphere microbiology to study microbial behaviour on plant leaf surfaces. These surfaces enable a reductionist approach to be undertaken, to enable individual environmental factors influencing microorganisms to be studied. Commonly used artificial surfaces include nutrient agar, isolated leaf cuticles, and reconstituted leaf waxes. Recently, replica surfaces mimicking the complex topography of leaf surfaces for phyllosphere microbiology studies are appearing in literature. Replica leaf surfaces have been produced in agar, epoxy, polystyrene, and polydimethylsiloxane (PDMS). However, none of these protocols are suitable for replicating fragile leaves such as of the model plant Arabidopsis thaliana. This is of importance, as A. thaliana is a model system for molecular plant genetics, molecular plant biology, and microbial ecology. To overcome this limitation, we introduce a versatile replication protocol for replicating fragile leaf surfaces into PDMS. Here we demonstrate the capacity of our replication process using optical microscopy, atomic force microscopy (AFM), and contact angle measurements to compare living and PDMS replica A. thaliana leaf surfaces. To highlight the use of our replica leaf surfaces for phyllosphere microbiology, we visualise bacteria on the replica leaf surfaces in comparison to living leaf surfaces. |
format | Online Article Text |
id | pubmed-6783459 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-67834592019-10-16 Replicating Arabidopsis Model Leaf Surfaces for Phyllosphere Microbiology Soffe, Rebecca Bernach, Michal Remus-Emsermann, Mitja N. P. Nock, Volker Sci Rep Article Artificial surfaces are commonly used in place of leaves in phyllosphere microbiology to study microbial behaviour on plant leaf surfaces. These surfaces enable a reductionist approach to be undertaken, to enable individual environmental factors influencing microorganisms to be studied. Commonly used artificial surfaces include nutrient agar, isolated leaf cuticles, and reconstituted leaf waxes. Recently, replica surfaces mimicking the complex topography of leaf surfaces for phyllosphere microbiology studies are appearing in literature. Replica leaf surfaces have been produced in agar, epoxy, polystyrene, and polydimethylsiloxane (PDMS). However, none of these protocols are suitable for replicating fragile leaves such as of the model plant Arabidopsis thaliana. This is of importance, as A. thaliana is a model system for molecular plant genetics, molecular plant biology, and microbial ecology. To overcome this limitation, we introduce a versatile replication protocol for replicating fragile leaf surfaces into PDMS. Here we demonstrate the capacity of our replication process using optical microscopy, atomic force microscopy (AFM), and contact angle measurements to compare living and PDMS replica A. thaliana leaf surfaces. To highlight the use of our replica leaf surfaces for phyllosphere microbiology, we visualise bacteria on the replica leaf surfaces in comparison to living leaf surfaces. Nature Publishing Group UK 2019-10-08 /pmc/articles/PMC6783459/ /pubmed/31595008 http://dx.doi.org/10.1038/s41598-019-50983-7 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Soffe, Rebecca Bernach, Michal Remus-Emsermann, Mitja N. P. Nock, Volker Replicating Arabidopsis Model Leaf Surfaces for Phyllosphere Microbiology |
title | Replicating Arabidopsis Model Leaf Surfaces for Phyllosphere Microbiology |
title_full | Replicating Arabidopsis Model Leaf Surfaces for Phyllosphere Microbiology |
title_fullStr | Replicating Arabidopsis Model Leaf Surfaces for Phyllosphere Microbiology |
title_full_unstemmed | Replicating Arabidopsis Model Leaf Surfaces for Phyllosphere Microbiology |
title_short | Replicating Arabidopsis Model Leaf Surfaces for Phyllosphere Microbiology |
title_sort | replicating arabidopsis model leaf surfaces for phyllosphere microbiology |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6783459/ https://www.ncbi.nlm.nih.gov/pubmed/31595008 http://dx.doi.org/10.1038/s41598-019-50983-7 |
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