Photoactivation of silicon rhodamines via a light-induced protonation

Photoactivatable fluorophores are important for single-particle tracking and super-resolution microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent protonation. In contrast to other photoactivatable fluorophores, no cagi...

Descripción completa

Detalles Bibliográficos
Autores principales: Frei, Michelle S., Hoess, Philipp, Lampe, Marko, Nijmeijer, Bianca, Kueblbeck, Moritz, Ellenberg, Jan, Wadepohl, Hubert, Ries, Jonas, Pitsch, Stefan, Reymond, Luc, Johnsson, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6783549/
https://www.ncbi.nlm.nih.gov/pubmed/31594948
http://dx.doi.org/10.1038/s41467-019-12480-3
_version_ 1783457579927601152
author Frei, Michelle S.
Hoess, Philipp
Lampe, Marko
Nijmeijer, Bianca
Kueblbeck, Moritz
Ellenberg, Jan
Wadepohl, Hubert
Ries, Jonas
Pitsch, Stefan
Reymond, Luc
Johnsson, Kai
author_facet Frei, Michelle S.
Hoess, Philipp
Lampe, Marko
Nijmeijer, Bianca
Kueblbeck, Moritz
Ellenberg, Jan
Wadepohl, Hubert
Ries, Jonas
Pitsch, Stefan
Reymond, Luc
Johnsson, Kai
author_sort Frei, Michelle S.
collection PubMed
description Photoactivatable fluorophores are important for single-particle tracking and super-resolution microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent protonation. In contrast to other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we create probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore’s outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy.
format Online
Article
Text
id pubmed-6783549
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-67835492019-10-10 Photoactivation of silicon rhodamines via a light-induced protonation Frei, Michelle S. Hoess, Philipp Lampe, Marko Nijmeijer, Bianca Kueblbeck, Moritz Ellenberg, Jan Wadepohl, Hubert Ries, Jonas Pitsch, Stefan Reymond, Luc Johnsson, Kai Nat Commun Article Photoactivatable fluorophores are important for single-particle tracking and super-resolution microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent protonation. In contrast to other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we create probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore’s outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy. Nature Publishing Group UK 2019-10-08 /pmc/articles/PMC6783549/ /pubmed/31594948 http://dx.doi.org/10.1038/s41467-019-12480-3 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Frei, Michelle S.
Hoess, Philipp
Lampe, Marko
Nijmeijer, Bianca
Kueblbeck, Moritz
Ellenberg, Jan
Wadepohl, Hubert
Ries, Jonas
Pitsch, Stefan
Reymond, Luc
Johnsson, Kai
Photoactivation of silicon rhodamines via a light-induced protonation
title Photoactivation of silicon rhodamines via a light-induced protonation
title_full Photoactivation of silicon rhodamines via a light-induced protonation
title_fullStr Photoactivation of silicon rhodamines via a light-induced protonation
title_full_unstemmed Photoactivation of silicon rhodamines via a light-induced protonation
title_short Photoactivation of silicon rhodamines via a light-induced protonation
title_sort photoactivation of silicon rhodamines via a light-induced protonation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6783549/
https://www.ncbi.nlm.nih.gov/pubmed/31594948
http://dx.doi.org/10.1038/s41467-019-12480-3
work_keys_str_mv AT freimichelles photoactivationofsiliconrhodaminesviaalightinducedprotonation
AT hoessphilipp photoactivationofsiliconrhodaminesviaalightinducedprotonation
AT lampemarko photoactivationofsiliconrhodaminesviaalightinducedprotonation
AT nijmeijerbianca photoactivationofsiliconrhodaminesviaalightinducedprotonation
AT kueblbeckmoritz photoactivationofsiliconrhodaminesviaalightinducedprotonation
AT ellenbergjan photoactivationofsiliconrhodaminesviaalightinducedprotonation
AT wadepohlhubert photoactivationofsiliconrhodaminesviaalightinducedprotonation
AT riesjonas photoactivationofsiliconrhodaminesviaalightinducedprotonation
AT pitschstefan photoactivationofsiliconrhodaminesviaalightinducedprotonation
AT reymondluc photoactivationofsiliconrhodaminesviaalightinducedprotonation
AT johnssonkai photoactivationofsiliconrhodaminesviaalightinducedprotonation

Ejemplares similares