Cargando…
A circRNA signature predicts postoperative recurrence in stage II/III colon cancer
Accurate risk stratification for patients with stage II/III colon cancer is pivotal for postoperative treatment decisions. Here, we aimed to identify and validate a circRNA‐based signature that could improve postoperative prognostic stratification for these patients. In current retrospective analysi...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6783650/ https://www.ncbi.nlm.nih.gov/pubmed/31475771 http://dx.doi.org/10.15252/emmm.201810168 |
_version_ | 1783457594388512768 |
---|---|
author | Ju, Huai‐Qiang Zhao, Qi Wang, Feng Lan, Ping Wang, Zixian Zuo, Zhi‐Xiang Wu, Qi‐Nian Fan, Xin‐Juan Mo, Hai‐Yu Chen, Li Li, Ting Ren, Chao Wan, Xiang‐Bo Chen, Gong Li, Yu‐Hong Jia, Wei‐Hua Xu, Rui‐Hua |
author_facet | Ju, Huai‐Qiang Zhao, Qi Wang, Feng Lan, Ping Wang, Zixian Zuo, Zhi‐Xiang Wu, Qi‐Nian Fan, Xin‐Juan Mo, Hai‐Yu Chen, Li Li, Ting Ren, Chao Wan, Xiang‐Bo Chen, Gong Li, Yu‐Hong Jia, Wei‐Hua Xu, Rui‐Hua |
author_sort | Ju, Huai‐Qiang |
collection | PubMed |
description | Accurate risk stratification for patients with stage II/III colon cancer is pivotal for postoperative treatment decisions. Here, we aimed to identify and validate a circRNA‐based signature that could improve postoperative prognostic stratification for these patients. In current retrospective analysis, we included 667 patients with R0 resected stage II/III colon cancer. Using RNA‐seq analysis of 20 paired frozen tissues collected postoperation, we profiled differential circRNA expression between patients with and without recurrence, followed by quantitative validation. With clinical information, we generated a four‐circRNA‐based cirScore to classify patients into high‐risk and low‐risk groups in the training cohort. The patients with high cirScores in the training cohort had a shorter disease‐free survival (DFS) and overall survival (OS) than patients with low cirScores. The prognostic capacity of the classifier was validated in the internal and external cohorts. Loss‐of‐function assays indicated that the selected circRNAs played functional roles in colon cancer progression. Overall, our four‐circRNA‐based classifier is a reliable prognostic tool for postoperative disease recurrence in patients with stage II/III colon cancer. |
format | Online Article Text |
id | pubmed-6783650 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67836502019-10-17 A circRNA signature predicts postoperative recurrence in stage II/III colon cancer Ju, Huai‐Qiang Zhao, Qi Wang, Feng Lan, Ping Wang, Zixian Zuo, Zhi‐Xiang Wu, Qi‐Nian Fan, Xin‐Juan Mo, Hai‐Yu Chen, Li Li, Ting Ren, Chao Wan, Xiang‐Bo Chen, Gong Li, Yu‐Hong Jia, Wei‐Hua Xu, Rui‐Hua EMBO Mol Med Articles Accurate risk stratification for patients with stage II/III colon cancer is pivotal for postoperative treatment decisions. Here, we aimed to identify and validate a circRNA‐based signature that could improve postoperative prognostic stratification for these patients. In current retrospective analysis, we included 667 patients with R0 resected stage II/III colon cancer. Using RNA‐seq analysis of 20 paired frozen tissues collected postoperation, we profiled differential circRNA expression between patients with and without recurrence, followed by quantitative validation. With clinical information, we generated a four‐circRNA‐based cirScore to classify patients into high‐risk and low‐risk groups in the training cohort. The patients with high cirScores in the training cohort had a shorter disease‐free survival (DFS) and overall survival (OS) than patients with low cirScores. The prognostic capacity of the classifier was validated in the internal and external cohorts. Loss‐of‐function assays indicated that the selected circRNAs played functional roles in colon cancer progression. Overall, our four‐circRNA‐based classifier is a reliable prognostic tool for postoperative disease recurrence in patients with stage II/III colon cancer. John Wiley and Sons Inc. 2019-09-02 2019-10 /pmc/articles/PMC6783650/ /pubmed/31475771 http://dx.doi.org/10.15252/emmm.201810168 Text en © 2019 The Authors. Published under the terms of the CC BY 4.0 license This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Ju, Huai‐Qiang Zhao, Qi Wang, Feng Lan, Ping Wang, Zixian Zuo, Zhi‐Xiang Wu, Qi‐Nian Fan, Xin‐Juan Mo, Hai‐Yu Chen, Li Li, Ting Ren, Chao Wan, Xiang‐Bo Chen, Gong Li, Yu‐Hong Jia, Wei‐Hua Xu, Rui‐Hua A circRNA signature predicts postoperative recurrence in stage II/III colon cancer |
title | A circRNA signature predicts postoperative recurrence in stage II/III colon cancer |
title_full | A circRNA signature predicts postoperative recurrence in stage II/III colon cancer |
title_fullStr | A circRNA signature predicts postoperative recurrence in stage II/III colon cancer |
title_full_unstemmed | A circRNA signature predicts postoperative recurrence in stage II/III colon cancer |
title_short | A circRNA signature predicts postoperative recurrence in stage II/III colon cancer |
title_sort | circrna signature predicts postoperative recurrence in stage ii/iii colon cancer |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6783650/ https://www.ncbi.nlm.nih.gov/pubmed/31475771 http://dx.doi.org/10.15252/emmm.201810168 |
work_keys_str_mv | AT juhuaiqiang acircrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT zhaoqi acircrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT wangfeng acircrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT lanping acircrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT wangzixian acircrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT zuozhixiang acircrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT wuqinian acircrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT fanxinjuan acircrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT mohaiyu acircrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT chenli acircrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT liting acircrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT renchao acircrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT wanxiangbo acircrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT chengong acircrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT liyuhong acircrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT jiaweihua acircrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT xuruihua acircrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT juhuaiqiang circrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT zhaoqi circrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT wangfeng circrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT lanping circrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT wangzixian circrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT zuozhixiang circrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT wuqinian circrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT fanxinjuan circrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT mohaiyu circrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT chenli circrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT liting circrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT renchao circrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT wanxiangbo circrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT chengong circrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT liyuhong circrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT jiaweihua circrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer AT xuruihua circrnasignaturepredictspostoperativerecurrenceinstageiiiiicoloncancer |