Cargando…
Synergistic Action of Phage and Antibiotics: Parameters to Enhance the Killing Efficacy Against Mono and Dual-Species Biofilms
Pseudomonas aeruginosa and Staphylococcus aureus are opportunistic pathogens and are commonly found in polymicrobial biofilm-associated diseases, namely chronic wounds. Their co-existence in a biofilm contributes to an increased tolerance of the biofilm to antibiotics. Combined treatments of bacteri...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6783858/ https://www.ncbi.nlm.nih.gov/pubmed/31349628 http://dx.doi.org/10.3390/antibiotics8030103 |
_version_ | 1783457620667924480 |
---|---|
author | Akturk, Ergun Oliveira, Hugo Santos, Sílvio B. Costa, Susana Kuyumcu, Suleyman Melo, Luís D. R. Azeredo, Joana |
author_facet | Akturk, Ergun Oliveira, Hugo Santos, Sílvio B. Costa, Susana Kuyumcu, Suleyman Melo, Luís D. R. Azeredo, Joana |
author_sort | Akturk, Ergun |
collection | PubMed |
description | Pseudomonas aeruginosa and Staphylococcus aureus are opportunistic pathogens and are commonly found in polymicrobial biofilm-associated diseases, namely chronic wounds. Their co-existence in a biofilm contributes to an increased tolerance of the biofilm to antibiotics. Combined treatments of bacteriophages and antibiotics have shown a promising antibiofilm activity, due to the profound differences in their mechanisms of action. In this study, 48 h old mono and dual-species biofilms were treated with a newly isolated P. aeruginosa infecting phage (EPA1) and seven different antibiotics (gentamicin, kanamycin, tetracycline, chloramphenicol, erythromycin, ciprofloxacin, and meropenem), alone and in simultaneous or sequential combinations. The therapeutic efficacy of the tested antimicrobials was determined. Phage or antibiotics alone had a modest effect in reducing biofilm bacteria. However, when applied simultaneously, a profound improvement in the killing effect was observed. Moreover, an impressive biofilm reduction (below the detection limit) was observed when gentamicin or ciprofloxacin were added sequentially after 6 h of phage treatment. The effect observed does not depend on the type of antibiotic but is influenced by its concentration. Moreover, in dual-species biofilms it was necessary to increase gentamicin concentration to obtain a similar killing effect as occurs in mono-species. Overall, combining phages with antibiotics can be synergistic in reducing the bacterial density in biofilms. However, the concentration of antibiotic and the time of antibiotic application are essential factors that need to be considered in the combined treatments. |
format | Online Article Text |
id | pubmed-6783858 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67838582019-10-16 Synergistic Action of Phage and Antibiotics: Parameters to Enhance the Killing Efficacy Against Mono and Dual-Species Biofilms Akturk, Ergun Oliveira, Hugo Santos, Sílvio B. Costa, Susana Kuyumcu, Suleyman Melo, Luís D. R. Azeredo, Joana Antibiotics (Basel) Article Pseudomonas aeruginosa and Staphylococcus aureus are opportunistic pathogens and are commonly found in polymicrobial biofilm-associated diseases, namely chronic wounds. Their co-existence in a biofilm contributes to an increased tolerance of the biofilm to antibiotics. Combined treatments of bacteriophages and antibiotics have shown a promising antibiofilm activity, due to the profound differences in their mechanisms of action. In this study, 48 h old mono and dual-species biofilms were treated with a newly isolated P. aeruginosa infecting phage (EPA1) and seven different antibiotics (gentamicin, kanamycin, tetracycline, chloramphenicol, erythromycin, ciprofloxacin, and meropenem), alone and in simultaneous or sequential combinations. The therapeutic efficacy of the tested antimicrobials was determined. Phage or antibiotics alone had a modest effect in reducing biofilm bacteria. However, when applied simultaneously, a profound improvement in the killing effect was observed. Moreover, an impressive biofilm reduction (below the detection limit) was observed when gentamicin or ciprofloxacin were added sequentially after 6 h of phage treatment. The effect observed does not depend on the type of antibiotic but is influenced by its concentration. Moreover, in dual-species biofilms it was necessary to increase gentamicin concentration to obtain a similar killing effect as occurs in mono-species. Overall, combining phages with antibiotics can be synergistic in reducing the bacterial density in biofilms. However, the concentration of antibiotic and the time of antibiotic application are essential factors that need to be considered in the combined treatments. MDPI 2019-07-25 /pmc/articles/PMC6783858/ /pubmed/31349628 http://dx.doi.org/10.3390/antibiotics8030103 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Akturk, Ergun Oliveira, Hugo Santos, Sílvio B. Costa, Susana Kuyumcu, Suleyman Melo, Luís D. R. Azeredo, Joana Synergistic Action of Phage and Antibiotics: Parameters to Enhance the Killing Efficacy Against Mono and Dual-Species Biofilms |
title | Synergistic Action of Phage and Antibiotics: Parameters to Enhance the Killing Efficacy Against Mono and Dual-Species Biofilms |
title_full | Synergistic Action of Phage and Antibiotics: Parameters to Enhance the Killing Efficacy Against Mono and Dual-Species Biofilms |
title_fullStr | Synergistic Action of Phage and Antibiotics: Parameters to Enhance the Killing Efficacy Against Mono and Dual-Species Biofilms |
title_full_unstemmed | Synergistic Action of Phage and Antibiotics: Parameters to Enhance the Killing Efficacy Against Mono and Dual-Species Biofilms |
title_short | Synergistic Action of Phage and Antibiotics: Parameters to Enhance the Killing Efficacy Against Mono and Dual-Species Biofilms |
title_sort | synergistic action of phage and antibiotics: parameters to enhance the killing efficacy against mono and dual-species biofilms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6783858/ https://www.ncbi.nlm.nih.gov/pubmed/31349628 http://dx.doi.org/10.3390/antibiotics8030103 |
work_keys_str_mv | AT akturkergun synergisticactionofphageandantibioticsparameterstoenhancethekillingefficacyagainstmonoanddualspeciesbiofilms AT oliveirahugo synergisticactionofphageandantibioticsparameterstoenhancethekillingefficacyagainstmonoanddualspeciesbiofilms AT santossilviob synergisticactionofphageandantibioticsparameterstoenhancethekillingefficacyagainstmonoanddualspeciesbiofilms AT costasusana synergisticactionofphageandantibioticsparameterstoenhancethekillingefficacyagainstmonoanddualspeciesbiofilms AT kuyumcusuleyman synergisticactionofphageandantibioticsparameterstoenhancethekillingefficacyagainstmonoanddualspeciesbiofilms AT meloluisdr synergisticactionofphageandantibioticsparameterstoenhancethekillingefficacyagainstmonoanddualspeciesbiofilms AT azeredojoana synergisticactionofphageandantibioticsparameterstoenhancethekillingefficacyagainstmonoanddualspeciesbiofilms |