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Diverse Morphology and Structural Features of Old and New World Hantaviruses

To further understanding of the structure and morphology of the Orthohantavirus, family Hantaviridae, we have employed cryo-electron microscopy (cryo-EM) for three New World hantaviruses: Andes (ANDV), Sin Nombre (SNV), and Black Creek Canal (BCCV). Building upon our prior cryo-EM and cryo-tomograph...

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Autores principales: Parvate, Amar, Williams, Evan P., Taylor, Mariah K., Chu, Yong-Kyu, Lanman, Jason, Saphire, Erica Ollmann, Jonsson, Colleen B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6783877/
https://www.ncbi.nlm.nih.gov/pubmed/31527500
http://dx.doi.org/10.3390/v11090862
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author Parvate, Amar
Williams, Evan P.
Taylor, Mariah K.
Chu, Yong-Kyu
Lanman, Jason
Saphire, Erica Ollmann
Jonsson, Colleen B.
author_facet Parvate, Amar
Williams, Evan P.
Taylor, Mariah K.
Chu, Yong-Kyu
Lanman, Jason
Saphire, Erica Ollmann
Jonsson, Colleen B.
author_sort Parvate, Amar
collection PubMed
description To further understanding of the structure and morphology of the Orthohantavirus, family Hantaviridae, we have employed cryo-electron microscopy (cryo-EM) for three New World hantaviruses: Andes (ANDV), Sin Nombre (SNV), and Black Creek Canal (BCCV). Building upon our prior cryo-EM and cryo-tomography study of the Old World hantavirus, Hantaan virus (HTNV), we have expanded our studies to examine the entire virion population present in cell culture supernatant. Hence, in contrast to the prior cryo-EM/ET studies in which we used a polyethylene precipitation, a sucrose gradient, and a sucrose cushion, we used two sucrose cushions. We inactivated the material after the first cushion. We tested the method using HTNV which has a known cryo-EM structure and observed equivalent results. Therefore, we used this method to assess the particle distribution of the New World hantaviruses by cryo-EM. Cryo-EM images showed a diverse range of sizes and morphologies for the New World viruses that we classified as round, tubular, and irregular. Strikingly, BCCV virions were mostly tubular. These first cryo-EM images of the New World Orthohantavirus confirm prior EM observations that noted tubular projections of SNV at the plasma membrane during virion morphogenesis but were not confirmed. These findings underscore the need for further investigation of virion morphogenesis of the Orthohantavirus.
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spelling pubmed-67838772019-10-16 Diverse Morphology and Structural Features of Old and New World Hantaviruses Parvate, Amar Williams, Evan P. Taylor, Mariah K. Chu, Yong-Kyu Lanman, Jason Saphire, Erica Ollmann Jonsson, Colleen B. Viruses Article To further understanding of the structure and morphology of the Orthohantavirus, family Hantaviridae, we have employed cryo-electron microscopy (cryo-EM) for three New World hantaviruses: Andes (ANDV), Sin Nombre (SNV), and Black Creek Canal (BCCV). Building upon our prior cryo-EM and cryo-tomography study of the Old World hantavirus, Hantaan virus (HTNV), we have expanded our studies to examine the entire virion population present in cell culture supernatant. Hence, in contrast to the prior cryo-EM/ET studies in which we used a polyethylene precipitation, a sucrose gradient, and a sucrose cushion, we used two sucrose cushions. We inactivated the material after the first cushion. We tested the method using HTNV which has a known cryo-EM structure and observed equivalent results. Therefore, we used this method to assess the particle distribution of the New World hantaviruses by cryo-EM. Cryo-EM images showed a diverse range of sizes and morphologies for the New World viruses that we classified as round, tubular, and irregular. Strikingly, BCCV virions were mostly tubular. These first cryo-EM images of the New World Orthohantavirus confirm prior EM observations that noted tubular projections of SNV at the plasma membrane during virion morphogenesis but were not confirmed. These findings underscore the need for further investigation of virion morphogenesis of the Orthohantavirus. MDPI 2019-09-16 /pmc/articles/PMC6783877/ /pubmed/31527500 http://dx.doi.org/10.3390/v11090862 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Parvate, Amar
Williams, Evan P.
Taylor, Mariah K.
Chu, Yong-Kyu
Lanman, Jason
Saphire, Erica Ollmann
Jonsson, Colleen B.
Diverse Morphology and Structural Features of Old and New World Hantaviruses
title Diverse Morphology and Structural Features of Old and New World Hantaviruses
title_full Diverse Morphology and Structural Features of Old and New World Hantaviruses
title_fullStr Diverse Morphology and Structural Features of Old and New World Hantaviruses
title_full_unstemmed Diverse Morphology and Structural Features of Old and New World Hantaviruses
title_short Diverse Morphology and Structural Features of Old and New World Hantaviruses
title_sort diverse morphology and structural features of old and new world hantaviruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6783877/
https://www.ncbi.nlm.nih.gov/pubmed/31527500
http://dx.doi.org/10.3390/v11090862
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