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Antibiofilm Activity of Three Different Irrigation Techniques: An in Vitro Study

The microbial infection of the endodontic space occurs in a necrotic tooth as a result of dental caries, trauma, periodontal disease, or previous root canal therapy. The disruption of the biofilms and the reduction of the bacterial load inside root canals are crucial for the success of root canal th...

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Autores principales: Eneide, Caterina, Castagnola, Raffaella, Martini, Cecilia, Grande, Nicola Maria, Bugli, Francesca, Patini, Romeo, Cordaro, Massimo, Sanguinetti, Maurizio, Olivi, Giovanni, Isola, Gaetano, Marigo, Luca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784003/
https://www.ncbi.nlm.nih.gov/pubmed/31405049
http://dx.doi.org/10.3390/antibiotics8030112
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author Eneide, Caterina
Castagnola, Raffaella
Martini, Cecilia
Grande, Nicola Maria
Bugli, Francesca
Patini, Romeo
Cordaro, Massimo
Sanguinetti, Maurizio
Olivi, Giovanni
Isola, Gaetano
Marigo, Luca
author_facet Eneide, Caterina
Castagnola, Raffaella
Martini, Cecilia
Grande, Nicola Maria
Bugli, Francesca
Patini, Romeo
Cordaro, Massimo
Sanguinetti, Maurizio
Olivi, Giovanni
Isola, Gaetano
Marigo, Luca
author_sort Eneide, Caterina
collection PubMed
description The microbial infection of the endodontic space occurs in a necrotic tooth as a result of dental caries, trauma, periodontal disease, or previous root canal therapy. The disruption of the biofilms and the reduction of the bacterial load inside root canals are crucial for the success of root canal therapy. The aim of this study was to compare, in vitro, the antibiofilm efficacy of a novel passive sonic irrigation (PSI) device with passive ultrasonic irrigation (PUI) and conventional needle irrigation (CNI). Forty-four single-rooted human teeth were inoculated with a culture of E. faecalis for 28 days. The specimens were randomly divided into three groups: PUI, CNI, and PSI (n = 12). The activation protocols were performed using both 17% EDTA and 5.25% NaOCl. Residual bacterial biofilm was taken by means of a canal brush and colony-forming unit (CFU) were counted. The data were analyzed using one-way ANOVA and Games-Howell’s post hoc tests. A major reduction in CFU was observed in the PSI and PUI groups, in comparison with the CNI group. No difference was found (p > 0.05) in terms of CFU reduction between PSI and PUI. PSI could be as effective as PUI in the removal of bacterial biofilms from straight root canals.
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spelling pubmed-67840032019-10-16 Antibiofilm Activity of Three Different Irrigation Techniques: An in Vitro Study Eneide, Caterina Castagnola, Raffaella Martini, Cecilia Grande, Nicola Maria Bugli, Francesca Patini, Romeo Cordaro, Massimo Sanguinetti, Maurizio Olivi, Giovanni Isola, Gaetano Marigo, Luca Antibiotics (Basel) Article The microbial infection of the endodontic space occurs in a necrotic tooth as a result of dental caries, trauma, periodontal disease, or previous root canal therapy. The disruption of the biofilms and the reduction of the bacterial load inside root canals are crucial for the success of root canal therapy. The aim of this study was to compare, in vitro, the antibiofilm efficacy of a novel passive sonic irrigation (PSI) device with passive ultrasonic irrigation (PUI) and conventional needle irrigation (CNI). Forty-four single-rooted human teeth were inoculated with a culture of E. faecalis for 28 days. The specimens were randomly divided into three groups: PUI, CNI, and PSI (n = 12). The activation protocols were performed using both 17% EDTA and 5.25% NaOCl. Residual bacterial biofilm was taken by means of a canal brush and colony-forming unit (CFU) were counted. The data were analyzed using one-way ANOVA and Games-Howell’s post hoc tests. A major reduction in CFU was observed in the PSI and PUI groups, in comparison with the CNI group. No difference was found (p > 0.05) in terms of CFU reduction between PSI and PUI. PSI could be as effective as PUI in the removal of bacterial biofilms from straight root canals. MDPI 2019-08-09 /pmc/articles/PMC6784003/ /pubmed/31405049 http://dx.doi.org/10.3390/antibiotics8030112 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Eneide, Caterina
Castagnola, Raffaella
Martini, Cecilia
Grande, Nicola Maria
Bugli, Francesca
Patini, Romeo
Cordaro, Massimo
Sanguinetti, Maurizio
Olivi, Giovanni
Isola, Gaetano
Marigo, Luca
Antibiofilm Activity of Three Different Irrigation Techniques: An in Vitro Study
title Antibiofilm Activity of Three Different Irrigation Techniques: An in Vitro Study
title_full Antibiofilm Activity of Three Different Irrigation Techniques: An in Vitro Study
title_fullStr Antibiofilm Activity of Three Different Irrigation Techniques: An in Vitro Study
title_full_unstemmed Antibiofilm Activity of Three Different Irrigation Techniques: An in Vitro Study
title_short Antibiofilm Activity of Three Different Irrigation Techniques: An in Vitro Study
title_sort antibiofilm activity of three different irrigation techniques: an in vitro study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784003/
https://www.ncbi.nlm.nih.gov/pubmed/31405049
http://dx.doi.org/10.3390/antibiotics8030112
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