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Reactive Oxygen Species Alleviate Cell Death Induced by Thaxtomin A in Arabidopsis thaliana Cell Cultures

Thaxtomin A (TA) is a cellulose biosynthesis inhibitor synthesized by the soil actinobacterium Streptomyces scabies, which is the main causal agent of potato common scab. TA is essential for the induction of scab lesions on potato tubers. When added to Arabidopsis thaliana cell cultures, TA induces...

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Autores principales: Awwad, Fatima, Bertrand, Guillaume, Grandbois, Michel, Beaudoin, Nathalie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784117/
https://www.ncbi.nlm.nih.gov/pubmed/31489878
http://dx.doi.org/10.3390/plants8090332
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author Awwad, Fatima
Bertrand, Guillaume
Grandbois, Michel
Beaudoin, Nathalie
author_facet Awwad, Fatima
Bertrand, Guillaume
Grandbois, Michel
Beaudoin, Nathalie
author_sort Awwad, Fatima
collection PubMed
description Thaxtomin A (TA) is a cellulose biosynthesis inhibitor synthesized by the soil actinobacterium Streptomyces scabies, which is the main causal agent of potato common scab. TA is essential for the induction of scab lesions on potato tubers. When added to Arabidopsis thaliana cell cultures, TA induces an atypical programmed cell death (PCD). Although production of reactive oxygen species (ROS) often correlates with the induction of PCD, we observed a decrease in ROS levels following TA treatment. We show that this decrease in ROS accumulation in TA-treated cells is not due to the activation of antioxidant enzymes. Moreover, Arabidopsis cell cultures treated with hydrogen peroxide (H(2)O(2)) prior to TA treatment had significantly fewer dead cells than cultures treated with TA alone. This suggests that H(2)O(2) induces biochemical or molecular changes in cell cultures that alleviate the activation of PCD by TA. Investigation of the cell wall mechanics using atomic force microscopy showed that H(2)O(2) treatment can prevent the decrease in cell wall rigidity observed after TA exposure. While we cannot exclude the possibility that H(2)O(2) may promote cell survival by altering the cellular redox environment or signaling pathways, our results suggest that H(2)O(2) may inhibit cell death, at least partially, by reinforcing the cell wall to prevent or compensate for damages induced by TA.
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spelling pubmed-67841172019-10-16 Reactive Oxygen Species Alleviate Cell Death Induced by Thaxtomin A in Arabidopsis thaliana Cell Cultures Awwad, Fatima Bertrand, Guillaume Grandbois, Michel Beaudoin, Nathalie Plants (Basel) Article Thaxtomin A (TA) is a cellulose biosynthesis inhibitor synthesized by the soil actinobacterium Streptomyces scabies, which is the main causal agent of potato common scab. TA is essential for the induction of scab lesions on potato tubers. When added to Arabidopsis thaliana cell cultures, TA induces an atypical programmed cell death (PCD). Although production of reactive oxygen species (ROS) often correlates with the induction of PCD, we observed a decrease in ROS levels following TA treatment. We show that this decrease in ROS accumulation in TA-treated cells is not due to the activation of antioxidant enzymes. Moreover, Arabidopsis cell cultures treated with hydrogen peroxide (H(2)O(2)) prior to TA treatment had significantly fewer dead cells than cultures treated with TA alone. This suggests that H(2)O(2) induces biochemical or molecular changes in cell cultures that alleviate the activation of PCD by TA. Investigation of the cell wall mechanics using atomic force microscopy showed that H(2)O(2) treatment can prevent the decrease in cell wall rigidity observed after TA exposure. While we cannot exclude the possibility that H(2)O(2) may promote cell survival by altering the cellular redox environment or signaling pathways, our results suggest that H(2)O(2) may inhibit cell death, at least partially, by reinforcing the cell wall to prevent or compensate for damages induced by TA. MDPI 2019-09-06 /pmc/articles/PMC6784117/ /pubmed/31489878 http://dx.doi.org/10.3390/plants8090332 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Awwad, Fatima
Bertrand, Guillaume
Grandbois, Michel
Beaudoin, Nathalie
Reactive Oxygen Species Alleviate Cell Death Induced by Thaxtomin A in Arabidopsis thaliana Cell Cultures
title Reactive Oxygen Species Alleviate Cell Death Induced by Thaxtomin A in Arabidopsis thaliana Cell Cultures
title_full Reactive Oxygen Species Alleviate Cell Death Induced by Thaxtomin A in Arabidopsis thaliana Cell Cultures
title_fullStr Reactive Oxygen Species Alleviate Cell Death Induced by Thaxtomin A in Arabidopsis thaliana Cell Cultures
title_full_unstemmed Reactive Oxygen Species Alleviate Cell Death Induced by Thaxtomin A in Arabidopsis thaliana Cell Cultures
title_short Reactive Oxygen Species Alleviate Cell Death Induced by Thaxtomin A in Arabidopsis thaliana Cell Cultures
title_sort reactive oxygen species alleviate cell death induced by thaxtomin a in arabidopsis thaliana cell cultures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784117/
https://www.ncbi.nlm.nih.gov/pubmed/31489878
http://dx.doi.org/10.3390/plants8090332
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