Cargando…

Infections of the Xylella fastidiosa subsp. pauca Strain “De Donno” in Alfalfa (Medicago sativa) Elicits an Overactive Immune Response

Diseases caused by Xylella fastidiosa are among the most destructive for several agricultural productions. A deadly disease of olive, termed olive quick decline syndrome, is one of the most recent examples of the severe impacts caused by the introduction and spread of this bacterium in new ecosystem...

Descripción completa

Detalles Bibliográficos
Autores principales: Abou Kubaa, Raied, Giampetruzzi, Annalisa, Altamura, Giuseppe, Saponari, Maria, Saldarelli, Pasquale
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784145/
https://www.ncbi.nlm.nih.gov/pubmed/31500293
http://dx.doi.org/10.3390/plants8090335
_version_ 1783457701283495936
author Abou Kubaa, Raied
Giampetruzzi, Annalisa
Altamura, Giuseppe
Saponari, Maria
Saldarelli, Pasquale
author_facet Abou Kubaa, Raied
Giampetruzzi, Annalisa
Altamura, Giuseppe
Saponari, Maria
Saldarelli, Pasquale
author_sort Abou Kubaa, Raied
collection PubMed
description Diseases caused by Xylella fastidiosa are among the most destructive for several agricultural productions. A deadly disease of olive, termed olive quick decline syndrome, is one of the most recent examples of the severe impacts caused by the introduction and spread of this bacterium in new ecosystems with favorable epidemiological conditions. Deciphering the cascade of events leading to the development of severe alterations in the susceptible host plants is a priority of several research programs investigating strategies to mitigate the detrimental impacts of the infections. However, in the case of olives, the long latent period (>1 year) makes this pathosystem not amenable for such studies. We have inoculated alfalfa (Medicago sativa) with the olive-infecting strain “De Donno” isolated from a symptomatic olive in Apulia (Italy), and we demonstrated that this highly pathogenic strain causes an overactive reaction that ends up with the necrosis of the inoculated stem, a reaction that differs from the notoriously Alfalfa Dwarf disease, caused by X. fastidiosa strains isolated from grapes and almonds. RNASeq analysis showed that major plant immunity pathways are activated, in particular, several calcium transmembrane transporters and enzymes responsible for the production of reactive oxygen species (ROS). Signs of the necrotic reaction are anticipated by the upregulation of genes responsible for plant cell death and the hypersensitive reaction. Overall the whole infection process takes four months in alfalfa, which makes this pathosystem suitable for studies involving either the plant response to the infection or the role of Xylella genes in the expression of symptoms.
format Online
Article
Text
id pubmed-6784145
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-67841452019-10-16 Infections of the Xylella fastidiosa subsp. pauca Strain “De Donno” in Alfalfa (Medicago sativa) Elicits an Overactive Immune Response Abou Kubaa, Raied Giampetruzzi, Annalisa Altamura, Giuseppe Saponari, Maria Saldarelli, Pasquale Plants (Basel) Article Diseases caused by Xylella fastidiosa are among the most destructive for several agricultural productions. A deadly disease of olive, termed olive quick decline syndrome, is one of the most recent examples of the severe impacts caused by the introduction and spread of this bacterium in new ecosystems with favorable epidemiological conditions. Deciphering the cascade of events leading to the development of severe alterations in the susceptible host plants is a priority of several research programs investigating strategies to mitigate the detrimental impacts of the infections. However, in the case of olives, the long latent period (>1 year) makes this pathosystem not amenable for such studies. We have inoculated alfalfa (Medicago sativa) with the olive-infecting strain “De Donno” isolated from a symptomatic olive in Apulia (Italy), and we demonstrated that this highly pathogenic strain causes an overactive reaction that ends up with the necrosis of the inoculated stem, a reaction that differs from the notoriously Alfalfa Dwarf disease, caused by X. fastidiosa strains isolated from grapes and almonds. RNASeq analysis showed that major plant immunity pathways are activated, in particular, several calcium transmembrane transporters and enzymes responsible for the production of reactive oxygen species (ROS). Signs of the necrotic reaction are anticipated by the upregulation of genes responsible for plant cell death and the hypersensitive reaction. Overall the whole infection process takes four months in alfalfa, which makes this pathosystem suitable for studies involving either the plant response to the infection or the role of Xylella genes in the expression of symptoms. MDPI 2019-09-07 /pmc/articles/PMC6784145/ /pubmed/31500293 http://dx.doi.org/10.3390/plants8090335 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Abou Kubaa, Raied
Giampetruzzi, Annalisa
Altamura, Giuseppe
Saponari, Maria
Saldarelli, Pasquale
Infections of the Xylella fastidiosa subsp. pauca Strain “De Donno” in Alfalfa (Medicago sativa) Elicits an Overactive Immune Response
title Infections of the Xylella fastidiosa subsp. pauca Strain “De Donno” in Alfalfa (Medicago sativa) Elicits an Overactive Immune Response
title_full Infections of the Xylella fastidiosa subsp. pauca Strain “De Donno” in Alfalfa (Medicago sativa) Elicits an Overactive Immune Response
title_fullStr Infections of the Xylella fastidiosa subsp. pauca Strain “De Donno” in Alfalfa (Medicago sativa) Elicits an Overactive Immune Response
title_full_unstemmed Infections of the Xylella fastidiosa subsp. pauca Strain “De Donno” in Alfalfa (Medicago sativa) Elicits an Overactive Immune Response
title_short Infections of the Xylella fastidiosa subsp. pauca Strain “De Donno” in Alfalfa (Medicago sativa) Elicits an Overactive Immune Response
title_sort infections of the xylella fastidiosa subsp. pauca strain “de donno” in alfalfa (medicago sativa) elicits an overactive immune response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784145/
https://www.ncbi.nlm.nih.gov/pubmed/31500293
http://dx.doi.org/10.3390/plants8090335
work_keys_str_mv AT aboukubaaraied infectionsofthexylellafastidiosasubsppaucastraindedonnoinalfalfamedicagosativaelicitsanoveractiveimmuneresponse
AT giampetruzziannalisa infectionsofthexylellafastidiosasubsppaucastraindedonnoinalfalfamedicagosativaelicitsanoveractiveimmuneresponse
AT altamuragiuseppe infectionsofthexylellafastidiosasubsppaucastraindedonnoinalfalfamedicagosativaelicitsanoveractiveimmuneresponse
AT saponarimaria infectionsofthexylellafastidiosasubsppaucastraindedonnoinalfalfamedicagosativaelicitsanoveractiveimmuneresponse
AT saldarellipasquale infectionsofthexylellafastidiosasubsppaucastraindedonnoinalfalfamedicagosativaelicitsanoveractiveimmuneresponse