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A Recombinant Turkey Herpesvirus Expressing F and HN Genes of Avian Avulavirus-1 (AAvV-1) Genotype VI Confers Cross-Protection against Challenge with Virulent AAvV-1 Genotypes IV and VII in Chickens

Newcastle disease (ND) is responsible for significant economic losses in the poultry industry. The disease is caused by virulent strains of Avian avulavirus 1 (AAvV-1), a species within the family Paramyxoviridae. We developed a recombinant construct based on the herpesvirus of turkeys (HVT) as a ve...

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Autores principales: Śmietanka, Krzysztof, Tyborowska, Jolanta, Olszewska-Tomczyk, Monika, Domańska-Blicharz, Katarzyna, Minta, Zenon, Rabalski, Lukasz, Czarnota, Anna, Kucharczyk, Krzysztof, Szewczyk, Boguslaw
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784189/
https://www.ncbi.nlm.nih.gov/pubmed/31450681
http://dx.doi.org/10.3390/v11090784
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author Śmietanka, Krzysztof
Tyborowska, Jolanta
Olszewska-Tomczyk, Monika
Domańska-Blicharz, Katarzyna
Minta, Zenon
Rabalski, Lukasz
Czarnota, Anna
Kucharczyk, Krzysztof
Szewczyk, Boguslaw
author_facet Śmietanka, Krzysztof
Tyborowska, Jolanta
Olszewska-Tomczyk, Monika
Domańska-Blicharz, Katarzyna
Minta, Zenon
Rabalski, Lukasz
Czarnota, Anna
Kucharczyk, Krzysztof
Szewczyk, Boguslaw
author_sort Śmietanka, Krzysztof
collection PubMed
description Newcastle disease (ND) is responsible for significant economic losses in the poultry industry. The disease is caused by virulent strains of Avian avulavirus 1 (AAvV-1), a species within the family Paramyxoviridae. We developed a recombinant construct based on the herpesvirus of turkeys (HVT) as a vector expressing two genes: F and HN (HVT-NDV-F-HN) derived from the AAvV-1 genotype VI (“pigeon variant” of AAvV-1). This recombinant viral vaccine candidate was used to subcutaneously immunize one group of specific pathogen-free (SPF) chickens and two groups of broiler chickens (20 one-day-old birds/group). Humoral immune response was evaluated by hemagglutination-inhibition test and enzyme-linked immunosorbent assay (ELISA). The efficacy of the immunization was assessed in two separate challenge studies performed at 6 weeks of age with the use of virulent AAvV-1 strains representing heterologous genotypes IV and VII. The developed vaccine candidate elicited complete protection in SPF chickens since none of the birds became sick or died during the 2-week observation period. In the broiler groups, 90% and 100% clinical protection were achieved after challenges with AAvV-1 of IV and VII genotypes, respectively. We found no obvious relationship between antibody levels and protection assessed in broilers in the challenge study. The developed recombinant HVT-NDV-F-HN construct containing genes from a genotype VI AAvV-1 offers promising results as a potential vaccine candidate against ND in chickens.
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spelling pubmed-67841892019-10-16 A Recombinant Turkey Herpesvirus Expressing F and HN Genes of Avian Avulavirus-1 (AAvV-1) Genotype VI Confers Cross-Protection against Challenge with Virulent AAvV-1 Genotypes IV and VII in Chickens Śmietanka, Krzysztof Tyborowska, Jolanta Olszewska-Tomczyk, Monika Domańska-Blicharz, Katarzyna Minta, Zenon Rabalski, Lukasz Czarnota, Anna Kucharczyk, Krzysztof Szewczyk, Boguslaw Viruses Article Newcastle disease (ND) is responsible for significant economic losses in the poultry industry. The disease is caused by virulent strains of Avian avulavirus 1 (AAvV-1), a species within the family Paramyxoviridae. We developed a recombinant construct based on the herpesvirus of turkeys (HVT) as a vector expressing two genes: F and HN (HVT-NDV-F-HN) derived from the AAvV-1 genotype VI (“pigeon variant” of AAvV-1). This recombinant viral vaccine candidate was used to subcutaneously immunize one group of specific pathogen-free (SPF) chickens and two groups of broiler chickens (20 one-day-old birds/group). Humoral immune response was evaluated by hemagglutination-inhibition test and enzyme-linked immunosorbent assay (ELISA). The efficacy of the immunization was assessed in two separate challenge studies performed at 6 weeks of age with the use of virulent AAvV-1 strains representing heterologous genotypes IV and VII. The developed vaccine candidate elicited complete protection in SPF chickens since none of the birds became sick or died during the 2-week observation period. In the broiler groups, 90% and 100% clinical protection were achieved after challenges with AAvV-1 of IV and VII genotypes, respectively. We found no obvious relationship between antibody levels and protection assessed in broilers in the challenge study. The developed recombinant HVT-NDV-F-HN construct containing genes from a genotype VI AAvV-1 offers promising results as a potential vaccine candidate against ND in chickens. MDPI 2019-08-25 /pmc/articles/PMC6784189/ /pubmed/31450681 http://dx.doi.org/10.3390/v11090784 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Śmietanka, Krzysztof
Tyborowska, Jolanta
Olszewska-Tomczyk, Monika
Domańska-Blicharz, Katarzyna
Minta, Zenon
Rabalski, Lukasz
Czarnota, Anna
Kucharczyk, Krzysztof
Szewczyk, Boguslaw
A Recombinant Turkey Herpesvirus Expressing F and HN Genes of Avian Avulavirus-1 (AAvV-1) Genotype VI Confers Cross-Protection against Challenge with Virulent AAvV-1 Genotypes IV and VII in Chickens
title A Recombinant Turkey Herpesvirus Expressing F and HN Genes of Avian Avulavirus-1 (AAvV-1) Genotype VI Confers Cross-Protection against Challenge with Virulent AAvV-1 Genotypes IV and VII in Chickens
title_full A Recombinant Turkey Herpesvirus Expressing F and HN Genes of Avian Avulavirus-1 (AAvV-1) Genotype VI Confers Cross-Protection against Challenge with Virulent AAvV-1 Genotypes IV and VII in Chickens
title_fullStr A Recombinant Turkey Herpesvirus Expressing F and HN Genes of Avian Avulavirus-1 (AAvV-1) Genotype VI Confers Cross-Protection against Challenge with Virulent AAvV-1 Genotypes IV and VII in Chickens
title_full_unstemmed A Recombinant Turkey Herpesvirus Expressing F and HN Genes of Avian Avulavirus-1 (AAvV-1) Genotype VI Confers Cross-Protection against Challenge with Virulent AAvV-1 Genotypes IV and VII in Chickens
title_short A Recombinant Turkey Herpesvirus Expressing F and HN Genes of Avian Avulavirus-1 (AAvV-1) Genotype VI Confers Cross-Protection against Challenge with Virulent AAvV-1 Genotypes IV and VII in Chickens
title_sort recombinant turkey herpesvirus expressing f and hn genes of avian avulavirus-1 (aavv-1) genotype vi confers cross-protection against challenge with virulent aavv-1 genotypes iv and vii in chickens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784189/
https://www.ncbi.nlm.nih.gov/pubmed/31450681
http://dx.doi.org/10.3390/v11090784
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