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Metaviromics Reveals Unknown Viral Diversity in the Biting Midge Culicoides impunctatus
Biting midges (Culicoides species) are vectors of arboviruses and were responsible for the emergence and spread of Schmallenberg virus (SBV) in Europe in 2011 and are likely to be involved in the emergence of other arboviruses in Europe. Improved surveillance and better understanding of risks requir...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784199/ https://www.ncbi.nlm.nih.gov/pubmed/31533247 http://dx.doi.org/10.3390/v11090865 |
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author | Modha, Sejal Hughes, Joseph Bianco, Giovanni Ferguson, Heather M. Helm, Barbara Tong, Lily Wilkie, Gavin S. Kohl, Alain Schnettler, Esther |
author_facet | Modha, Sejal Hughes, Joseph Bianco, Giovanni Ferguson, Heather M. Helm, Barbara Tong, Lily Wilkie, Gavin S. Kohl, Alain Schnettler, Esther |
author_sort | Modha, Sejal |
collection | PubMed |
description | Biting midges (Culicoides species) are vectors of arboviruses and were responsible for the emergence and spread of Schmallenberg virus (SBV) in Europe in 2011 and are likely to be involved in the emergence of other arboviruses in Europe. Improved surveillance and better understanding of risks require a better understanding of the circulating viral diversity in these biting insects. In this study, we expand the sequence space of RNA viruses by identifying a number of novel RNA viruses from Culicoides impunctatus (biting midge) using a meta-transcriptomic approach. A novel metaviromic pipeline called MetaViC was developed specifically to identify novel virus sequence signatures from high throughput sequencing (HTS) datasets in the absence of a known host genome. MetaViC is a protein centric pipeline that looks for specific protein signatures in the reads and contigs generated as part of the pipeline. Several novel viruses, including an alphanodavirus with both segments, a novel relative of the Hubei sobemo-like virus 49, two rhabdo-like viruses and a chuvirus, were identified in the Scottish midge samples. The newly identified viruses were found to be phylogenetically distinct to those previous known. These findings expand our current knowledge of viral diversity in arthropods and especially in these understudied disease vectors. |
format | Online Article Text |
id | pubmed-6784199 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67841992019-10-16 Metaviromics Reveals Unknown Viral Diversity in the Biting Midge Culicoides impunctatus Modha, Sejal Hughes, Joseph Bianco, Giovanni Ferguson, Heather M. Helm, Barbara Tong, Lily Wilkie, Gavin S. Kohl, Alain Schnettler, Esther Viruses Article Biting midges (Culicoides species) are vectors of arboviruses and were responsible for the emergence and spread of Schmallenberg virus (SBV) in Europe in 2011 and are likely to be involved in the emergence of other arboviruses in Europe. Improved surveillance and better understanding of risks require a better understanding of the circulating viral diversity in these biting insects. In this study, we expand the sequence space of RNA viruses by identifying a number of novel RNA viruses from Culicoides impunctatus (biting midge) using a meta-transcriptomic approach. A novel metaviromic pipeline called MetaViC was developed specifically to identify novel virus sequence signatures from high throughput sequencing (HTS) datasets in the absence of a known host genome. MetaViC is a protein centric pipeline that looks for specific protein signatures in the reads and contigs generated as part of the pipeline. Several novel viruses, including an alphanodavirus with both segments, a novel relative of the Hubei sobemo-like virus 49, two rhabdo-like viruses and a chuvirus, were identified in the Scottish midge samples. The newly identified viruses were found to be phylogenetically distinct to those previous known. These findings expand our current knowledge of viral diversity in arthropods and especially in these understudied disease vectors. MDPI 2019-09-17 /pmc/articles/PMC6784199/ /pubmed/31533247 http://dx.doi.org/10.3390/v11090865 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Modha, Sejal Hughes, Joseph Bianco, Giovanni Ferguson, Heather M. Helm, Barbara Tong, Lily Wilkie, Gavin S. Kohl, Alain Schnettler, Esther Metaviromics Reveals Unknown Viral Diversity in the Biting Midge Culicoides impunctatus |
title | Metaviromics Reveals Unknown Viral Diversity in the Biting Midge Culicoides impunctatus |
title_full | Metaviromics Reveals Unknown Viral Diversity in the Biting Midge Culicoides impunctatus |
title_fullStr | Metaviromics Reveals Unknown Viral Diversity in the Biting Midge Culicoides impunctatus |
title_full_unstemmed | Metaviromics Reveals Unknown Viral Diversity in the Biting Midge Culicoides impunctatus |
title_short | Metaviromics Reveals Unknown Viral Diversity in the Biting Midge Culicoides impunctatus |
title_sort | metaviromics reveals unknown viral diversity in the biting midge culicoides impunctatus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784199/ https://www.ncbi.nlm.nih.gov/pubmed/31533247 http://dx.doi.org/10.3390/v11090865 |
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