Cargando…

Cucurbit Chlorotic Yellows Virus p22 Protein Interacts with Cucumber SKP1LB1 and Its F-Box-Like Motif Is Crucial for Silencing Suppressor Activity

Plants use RNA silencing as a defense against viruses. In response, viruses encode various RNA silencing suppressors to counteract the antiviral silencing. Here, we identified p22 as a silencing suppressor of cucurbit chlorotic yellows crinivirus and showed that p22 interacts with CsSKP1LB1, a Cucum...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Siyu, Sun, Xinyan, Shi, Yajuan, Wei, Ying, Han, Xiaoyu, Li, Honglian, Chen, Linlin, Sun, Bingjian, Sun, Hangjun, Shi, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784205/
https://www.ncbi.nlm.nih.gov/pubmed/31487883
http://dx.doi.org/10.3390/v11090818
_version_ 1783457716330561536
author Chen, Siyu
Sun, Xinyan
Shi, Yajuan
Wei, Ying
Han, Xiaoyu
Li, Honglian
Chen, Linlin
Sun, Bingjian
Sun, Hangjun
Shi, Yan
author_facet Chen, Siyu
Sun, Xinyan
Shi, Yajuan
Wei, Ying
Han, Xiaoyu
Li, Honglian
Chen, Linlin
Sun, Bingjian
Sun, Hangjun
Shi, Yan
author_sort Chen, Siyu
collection PubMed
description Plants use RNA silencing as a defense against viruses. In response, viruses encode various RNA silencing suppressors to counteract the antiviral silencing. Here, we identified p22 as a silencing suppressor of cucurbit chlorotic yellows crinivirus and showed that p22 interacts with CsSKP1LB1, a Cucumis sativus ortholog of S-phase kinase-associated protein 1 (SKP1). The F-box-like motif of p22 was identified through sequence analysis and found to be necessary for the interaction using a yeast two-hybrid assay. The involvement of the F-box-like motif in p22 silencing suppressor activity was determined. Proteomics analysis of Nicotiana benthamiana leaves expressing p22, and its F-box-like motif deletion mutant showed 228 differentially expressed proteins and five enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways: ABC transporters, sesquiterpenoid and triterpenoid biosynthesis, ubiquitin-mediated proteolysis, riboflavin metabolism, and cysteine and methionine metabolism. Collectively, our results demonstrate the interaction between p22 and CsSKP1LB1 and show that the deletion of F-box-like motif inhibits p22 silencing suppressor activity. The possible pathways regulated by the p22 through the F-box-like motif were identified using proteomics analysis.
format Online
Article
Text
id pubmed-6784205
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-67842052019-10-16 Cucurbit Chlorotic Yellows Virus p22 Protein Interacts with Cucumber SKP1LB1 and Its F-Box-Like Motif Is Crucial for Silencing Suppressor Activity Chen, Siyu Sun, Xinyan Shi, Yajuan Wei, Ying Han, Xiaoyu Li, Honglian Chen, Linlin Sun, Bingjian Sun, Hangjun Shi, Yan Viruses Article Plants use RNA silencing as a defense against viruses. In response, viruses encode various RNA silencing suppressors to counteract the antiviral silencing. Here, we identified p22 as a silencing suppressor of cucurbit chlorotic yellows crinivirus and showed that p22 interacts with CsSKP1LB1, a Cucumis sativus ortholog of S-phase kinase-associated protein 1 (SKP1). The F-box-like motif of p22 was identified through sequence analysis and found to be necessary for the interaction using a yeast two-hybrid assay. The involvement of the F-box-like motif in p22 silencing suppressor activity was determined. Proteomics analysis of Nicotiana benthamiana leaves expressing p22, and its F-box-like motif deletion mutant showed 228 differentially expressed proteins and five enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways: ABC transporters, sesquiterpenoid and triterpenoid biosynthesis, ubiquitin-mediated proteolysis, riboflavin metabolism, and cysteine and methionine metabolism. Collectively, our results demonstrate the interaction between p22 and CsSKP1LB1 and show that the deletion of F-box-like motif inhibits p22 silencing suppressor activity. The possible pathways regulated by the p22 through the F-box-like motif were identified using proteomics analysis. MDPI 2019-09-04 /pmc/articles/PMC6784205/ /pubmed/31487883 http://dx.doi.org/10.3390/v11090818 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Siyu
Sun, Xinyan
Shi, Yajuan
Wei, Ying
Han, Xiaoyu
Li, Honglian
Chen, Linlin
Sun, Bingjian
Sun, Hangjun
Shi, Yan
Cucurbit Chlorotic Yellows Virus p22 Protein Interacts with Cucumber SKP1LB1 and Its F-Box-Like Motif Is Crucial for Silencing Suppressor Activity
title Cucurbit Chlorotic Yellows Virus p22 Protein Interacts with Cucumber SKP1LB1 and Its F-Box-Like Motif Is Crucial for Silencing Suppressor Activity
title_full Cucurbit Chlorotic Yellows Virus p22 Protein Interacts with Cucumber SKP1LB1 and Its F-Box-Like Motif Is Crucial for Silencing Suppressor Activity
title_fullStr Cucurbit Chlorotic Yellows Virus p22 Protein Interacts with Cucumber SKP1LB1 and Its F-Box-Like Motif Is Crucial for Silencing Suppressor Activity
title_full_unstemmed Cucurbit Chlorotic Yellows Virus p22 Protein Interacts with Cucumber SKP1LB1 and Its F-Box-Like Motif Is Crucial for Silencing Suppressor Activity
title_short Cucurbit Chlorotic Yellows Virus p22 Protein Interacts with Cucumber SKP1LB1 and Its F-Box-Like Motif Is Crucial for Silencing Suppressor Activity
title_sort cucurbit chlorotic yellows virus p22 protein interacts with cucumber skp1lb1 and its f-box-like motif is crucial for silencing suppressor activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784205/
https://www.ncbi.nlm.nih.gov/pubmed/31487883
http://dx.doi.org/10.3390/v11090818
work_keys_str_mv AT chensiyu cucurbitchloroticyellowsvirusp22proteininteractswithcucumberskp1lb1anditsfboxlikemotifiscrucialforsilencingsuppressoractivity
AT sunxinyan cucurbitchloroticyellowsvirusp22proteininteractswithcucumberskp1lb1anditsfboxlikemotifiscrucialforsilencingsuppressoractivity
AT shiyajuan cucurbitchloroticyellowsvirusp22proteininteractswithcucumberskp1lb1anditsfboxlikemotifiscrucialforsilencingsuppressoractivity
AT weiying cucurbitchloroticyellowsvirusp22proteininteractswithcucumberskp1lb1anditsfboxlikemotifiscrucialforsilencingsuppressoractivity
AT hanxiaoyu cucurbitchloroticyellowsvirusp22proteininteractswithcucumberskp1lb1anditsfboxlikemotifiscrucialforsilencingsuppressoractivity
AT lihonglian cucurbitchloroticyellowsvirusp22proteininteractswithcucumberskp1lb1anditsfboxlikemotifiscrucialforsilencingsuppressoractivity
AT chenlinlin cucurbitchloroticyellowsvirusp22proteininteractswithcucumberskp1lb1anditsfboxlikemotifiscrucialforsilencingsuppressoractivity
AT sunbingjian cucurbitchloroticyellowsvirusp22proteininteractswithcucumberskp1lb1anditsfboxlikemotifiscrucialforsilencingsuppressoractivity
AT sunhangjun cucurbitchloroticyellowsvirusp22proteininteractswithcucumberskp1lb1anditsfboxlikemotifiscrucialforsilencingsuppressoractivity
AT shiyan cucurbitchloroticyellowsvirusp22proteininteractswithcucumberskp1lb1anditsfboxlikemotifiscrucialforsilencingsuppressoractivity