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Intra-Sample Heterogeneity of Potato Starch Reveals Fluctuation of Starch-Binding Proteins According to Granule Morphology

Starch granule morphology is highly variable depending on the botanical origin. Moreover, all investigated plant species display intra-tissular variability of granule size. In potato tubers, the size distribution of starch granules follows a unimodal pattern with diameters ranging from 5 to 100 µm....

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Autores principales: Helle, Stanislas, Bray, Fabrice, Putaux, Jean-Luc, Verbeke, Jérémy, Flament, Stéphanie, Rolando, Christian, D’Hulst, Christophe, Szydlowski, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784226/
https://www.ncbi.nlm.nih.gov/pubmed/31487879
http://dx.doi.org/10.3390/plants8090324
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author Helle, Stanislas
Bray, Fabrice
Putaux, Jean-Luc
Verbeke, Jérémy
Flament, Stéphanie
Rolando, Christian
D’Hulst, Christophe
Szydlowski, Nicolas
author_facet Helle, Stanislas
Bray, Fabrice
Putaux, Jean-Luc
Verbeke, Jérémy
Flament, Stéphanie
Rolando, Christian
D’Hulst, Christophe
Szydlowski, Nicolas
author_sort Helle, Stanislas
collection PubMed
description Starch granule morphology is highly variable depending on the botanical origin. Moreover, all investigated plant species display intra-tissular variability of granule size. In potato tubers, the size distribution of starch granules follows a unimodal pattern with diameters ranging from 5 to 100 µm. Several evidences indicate that granule morphology in plants is related to the complex starch metabolic pathway. However, the intra-sample variability of starch-binding metabolic proteins remains unknown. Here, we report on the molecular characterization of size-fractionated potato starch granules with average diameters of 14.2 ± 3.7 µm, 24.5 ± 6.5 µm, 47.7 ± 12.8 µm, and 61.8 ± 17.4 µm. In addition to changes in the phosphate contents as well as small differences in the amylopectin structure, we found that the starch-binding protein stoichiometry varies significantly according to granule size. Label-free quantitative proteomics of each granule fraction revealed that individual proteins can be grouped according to four distinct abundance patterns. This study corroborates that the starch proteome may influence starch granule growth and architecture and opens up new perspectives in understanding the dynamics of starch biosynthesis.
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spelling pubmed-67842262019-10-16 Intra-Sample Heterogeneity of Potato Starch Reveals Fluctuation of Starch-Binding Proteins According to Granule Morphology Helle, Stanislas Bray, Fabrice Putaux, Jean-Luc Verbeke, Jérémy Flament, Stéphanie Rolando, Christian D’Hulst, Christophe Szydlowski, Nicolas Plants (Basel) Article Starch granule morphology is highly variable depending on the botanical origin. Moreover, all investigated plant species display intra-tissular variability of granule size. In potato tubers, the size distribution of starch granules follows a unimodal pattern with diameters ranging from 5 to 100 µm. Several evidences indicate that granule morphology in plants is related to the complex starch metabolic pathway. However, the intra-sample variability of starch-binding metabolic proteins remains unknown. Here, we report on the molecular characterization of size-fractionated potato starch granules with average diameters of 14.2 ± 3.7 µm, 24.5 ± 6.5 µm, 47.7 ± 12.8 µm, and 61.8 ± 17.4 µm. In addition to changes in the phosphate contents as well as small differences in the amylopectin structure, we found that the starch-binding protein stoichiometry varies significantly according to granule size. Label-free quantitative proteomics of each granule fraction revealed that individual proteins can be grouped according to four distinct abundance patterns. This study corroborates that the starch proteome may influence starch granule growth and architecture and opens up new perspectives in understanding the dynamics of starch biosynthesis. MDPI 2019-09-04 /pmc/articles/PMC6784226/ /pubmed/31487879 http://dx.doi.org/10.3390/plants8090324 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Helle, Stanislas
Bray, Fabrice
Putaux, Jean-Luc
Verbeke, Jérémy
Flament, Stéphanie
Rolando, Christian
D’Hulst, Christophe
Szydlowski, Nicolas
Intra-Sample Heterogeneity of Potato Starch Reveals Fluctuation of Starch-Binding Proteins According to Granule Morphology
title Intra-Sample Heterogeneity of Potato Starch Reveals Fluctuation of Starch-Binding Proteins According to Granule Morphology
title_full Intra-Sample Heterogeneity of Potato Starch Reveals Fluctuation of Starch-Binding Proteins According to Granule Morphology
title_fullStr Intra-Sample Heterogeneity of Potato Starch Reveals Fluctuation of Starch-Binding Proteins According to Granule Morphology
title_full_unstemmed Intra-Sample Heterogeneity of Potato Starch Reveals Fluctuation of Starch-Binding Proteins According to Granule Morphology
title_short Intra-Sample Heterogeneity of Potato Starch Reveals Fluctuation of Starch-Binding Proteins According to Granule Morphology
title_sort intra-sample heterogeneity of potato starch reveals fluctuation of starch-binding proteins according to granule morphology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784226/
https://www.ncbi.nlm.nih.gov/pubmed/31487879
http://dx.doi.org/10.3390/plants8090324
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