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Detection of Pig Cells Harboring Porcine Endogenous Retroviruses in Non-Human Primate Bladder After Renal Xenotransplantation

Pigs are used as potential donor animals for xenotransplantation. However, porcine endogenous retrovirus (PERV), shown to infect both human and non-human primate (NHP) cells in vitro, presents a risk of transmission to humans in xenotransplantation. In this study, we analyzed PERV transmission in va...

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Autores principales: Heo, Yoonki, Cho, Yeondong, Oh, Keon Bong, Park, Ki Hoon, Cho, Hansam, Choi, Hanul, Kim, Minjee, Yun, Ik Jin, Lee, Hee Jung, Kim, Young Bong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784250/
https://www.ncbi.nlm.nih.gov/pubmed/31470671
http://dx.doi.org/10.3390/v11090801
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author Heo, Yoonki
Cho, Yeondong
Oh, Keon Bong
Park, Ki Hoon
Cho, Hansam
Choi, Hanul
Kim, Minjee
Yun, Ik Jin
Lee, Hee Jung
Kim, Young Bong
author_facet Heo, Yoonki
Cho, Yeondong
Oh, Keon Bong
Park, Ki Hoon
Cho, Hansam
Choi, Hanul
Kim, Minjee
Yun, Ik Jin
Lee, Hee Jung
Kim, Young Bong
author_sort Heo, Yoonki
collection PubMed
description Pigs are used as potential donor animals for xenotransplantation. However, porcine endogenous retrovirus (PERV), shown to infect both human and non-human primate (NHP) cells in vitro, presents a risk of transmission to humans in xenotransplantation. In this study, we analyzed PERV transmission in various organs after pig-to-NHP xenotransplantation. We utilized pig-to-NHP xenotransplant tissue samples obtained using two types of transgenic pigs from the National Institute of Animal Science (NIAS, Republic of Korea), and examined them for the existence of PERV genes in different organs via PCR and RT-PCR with specific primers. To determine PERV insertion into chromosomes, inverse PCR using PERV long terminal repeat (LTR) region-specific primers was conducted. The PERV gene was not detected in NHP organs in cardiac xenotransplantation but detected in NHP bladders in renal xenotransplantation. The insertion experiment confirmed that PERVs originate from porcine donor cells rather than integrated provirus in the NHP chromosome. We also demonstrate the presence of pig cells in the NHP bladder after renal xenotransplantation using specific-porcine mitochondrial DNA gene PCR. The PERV sequence was detected in the bladder of NHPs after renal xenotransplantation by porcine cell-microchimerism but did not integrate into the NHP chromosome.
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spelling pubmed-67842502019-10-16 Detection of Pig Cells Harboring Porcine Endogenous Retroviruses in Non-Human Primate Bladder After Renal Xenotransplantation Heo, Yoonki Cho, Yeondong Oh, Keon Bong Park, Ki Hoon Cho, Hansam Choi, Hanul Kim, Minjee Yun, Ik Jin Lee, Hee Jung Kim, Young Bong Viruses Article Pigs are used as potential donor animals for xenotransplantation. However, porcine endogenous retrovirus (PERV), shown to infect both human and non-human primate (NHP) cells in vitro, presents a risk of transmission to humans in xenotransplantation. In this study, we analyzed PERV transmission in various organs after pig-to-NHP xenotransplantation. We utilized pig-to-NHP xenotransplant tissue samples obtained using two types of transgenic pigs from the National Institute of Animal Science (NIAS, Republic of Korea), and examined them for the existence of PERV genes in different organs via PCR and RT-PCR with specific primers. To determine PERV insertion into chromosomes, inverse PCR using PERV long terminal repeat (LTR) region-specific primers was conducted. The PERV gene was not detected in NHP organs in cardiac xenotransplantation but detected in NHP bladders in renal xenotransplantation. The insertion experiment confirmed that PERVs originate from porcine donor cells rather than integrated provirus in the NHP chromosome. We also demonstrate the presence of pig cells in the NHP bladder after renal xenotransplantation using specific-porcine mitochondrial DNA gene PCR. The PERV sequence was detected in the bladder of NHPs after renal xenotransplantation by porcine cell-microchimerism but did not integrate into the NHP chromosome. MDPI 2019-08-29 /pmc/articles/PMC6784250/ /pubmed/31470671 http://dx.doi.org/10.3390/v11090801 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Heo, Yoonki
Cho, Yeondong
Oh, Keon Bong
Park, Ki Hoon
Cho, Hansam
Choi, Hanul
Kim, Minjee
Yun, Ik Jin
Lee, Hee Jung
Kim, Young Bong
Detection of Pig Cells Harboring Porcine Endogenous Retroviruses in Non-Human Primate Bladder After Renal Xenotransplantation
title Detection of Pig Cells Harboring Porcine Endogenous Retroviruses in Non-Human Primate Bladder After Renal Xenotransplantation
title_full Detection of Pig Cells Harboring Porcine Endogenous Retroviruses in Non-Human Primate Bladder After Renal Xenotransplantation
title_fullStr Detection of Pig Cells Harboring Porcine Endogenous Retroviruses in Non-Human Primate Bladder After Renal Xenotransplantation
title_full_unstemmed Detection of Pig Cells Harboring Porcine Endogenous Retroviruses in Non-Human Primate Bladder After Renal Xenotransplantation
title_short Detection of Pig Cells Harboring Porcine Endogenous Retroviruses in Non-Human Primate Bladder After Renal Xenotransplantation
title_sort detection of pig cells harboring porcine endogenous retroviruses in non-human primate bladder after renal xenotransplantation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784250/
https://www.ncbi.nlm.nih.gov/pubmed/31470671
http://dx.doi.org/10.3390/v11090801
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