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Hepatitis C Core-Antigen Testing from Dried Blood Spots
In order to expand hepatitis C virus (HCV) screening, a change in the diagnostic paradigm is warranted to improve accessibility and decrease costs, such as utilizing dried blood spot (DBS) collection. In our study, blood from 68 patients with chronic HCV infection was spotted onto DBS cards and stor...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784259/ https://www.ncbi.nlm.nih.gov/pubmed/31489933 http://dx.doi.org/10.3390/v11090830 |
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author | Biondi, Mia J. van Tilborg, Marjolein Smookler, David Heymann, Gregory Aquino, Analiza Perusini, Stephen Mandel, Erin Kozak, Robert A. Cherepanov, Vera Kowgier, Matthew Hansen, Bettina Goneau, Lee W. Janssen, Harry L.A. Mazzulli, Tony Cloherty, Gavin de Knegt, Robert J. Feld, Jordan J. |
author_facet | Biondi, Mia J. van Tilborg, Marjolein Smookler, David Heymann, Gregory Aquino, Analiza Perusini, Stephen Mandel, Erin Kozak, Robert A. Cherepanov, Vera Kowgier, Matthew Hansen, Bettina Goneau, Lee W. Janssen, Harry L.A. Mazzulli, Tony Cloherty, Gavin de Knegt, Robert J. Feld, Jordan J. |
author_sort | Biondi, Mia J. |
collection | PubMed |
description | In order to expand hepatitis C virus (HCV) screening, a change in the diagnostic paradigm is warranted to improve accessibility and decrease costs, such as utilizing dried blood spot (DBS) collection. In our study, blood from 68 patients with chronic HCV infection was spotted onto DBS cards and stored at the following temperatures for one week: −80 °C, 4 °C, 21 °C, 37 °C, and alternating 37 °C and 4 °C; to assess whether temperature change during transportation would affect sensitivity. Sample was eluted from the DBS cards and tested for HCV antibodies (HCV-Ab) and HCV core antigen (core-Ag). HCV-Abs were detected from 68/68 DBS samples at −80 °C, 4 °C, 21 °C, and 67/68 at 37 °C and alternating 37 °C and 4 °C. Sensitivity of core-Ag was as follows: 94% (−80 °C), 94% (4 °C), 91% (21 °C), 93% (37 °C), and 93% (37 °C/4 °C). Not only did temperature not greatly affect sensitivity, but sensitivities are higher than previously reported, and support the use of this assay as an alternative to HCV RNA. We then completed a head-to-head comparison (n = 49) of venous versus capillary samples, and one versus two DBS. No difference in core-Ag sensitivity was observed by sample type, but there was an improvement when using two spots. We conclude that HCV-Abs and core-Ag testing from DBS cards has high diagnostic accuracy and could be considered as an alternative to HCV RNA in certain settings. |
format | Online Article Text |
id | pubmed-6784259 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67842592019-10-16 Hepatitis C Core-Antigen Testing from Dried Blood Spots Biondi, Mia J. van Tilborg, Marjolein Smookler, David Heymann, Gregory Aquino, Analiza Perusini, Stephen Mandel, Erin Kozak, Robert A. Cherepanov, Vera Kowgier, Matthew Hansen, Bettina Goneau, Lee W. Janssen, Harry L.A. Mazzulli, Tony Cloherty, Gavin de Knegt, Robert J. Feld, Jordan J. Viruses Article In order to expand hepatitis C virus (HCV) screening, a change in the diagnostic paradigm is warranted to improve accessibility and decrease costs, such as utilizing dried blood spot (DBS) collection. In our study, blood from 68 patients with chronic HCV infection was spotted onto DBS cards and stored at the following temperatures for one week: −80 °C, 4 °C, 21 °C, 37 °C, and alternating 37 °C and 4 °C; to assess whether temperature change during transportation would affect sensitivity. Sample was eluted from the DBS cards and tested for HCV antibodies (HCV-Ab) and HCV core antigen (core-Ag). HCV-Abs were detected from 68/68 DBS samples at −80 °C, 4 °C, 21 °C, and 67/68 at 37 °C and alternating 37 °C and 4 °C. Sensitivity of core-Ag was as follows: 94% (−80 °C), 94% (4 °C), 91% (21 °C), 93% (37 °C), and 93% (37 °C/4 °C). Not only did temperature not greatly affect sensitivity, but sensitivities are higher than previously reported, and support the use of this assay as an alternative to HCV RNA. We then completed a head-to-head comparison (n = 49) of venous versus capillary samples, and one versus two DBS. No difference in core-Ag sensitivity was observed by sample type, but there was an improvement when using two spots. We conclude that HCV-Abs and core-Ag testing from DBS cards has high diagnostic accuracy and could be considered as an alternative to HCV RNA in certain settings. MDPI 2019-09-06 /pmc/articles/PMC6784259/ /pubmed/31489933 http://dx.doi.org/10.3390/v11090830 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Biondi, Mia J. van Tilborg, Marjolein Smookler, David Heymann, Gregory Aquino, Analiza Perusini, Stephen Mandel, Erin Kozak, Robert A. Cherepanov, Vera Kowgier, Matthew Hansen, Bettina Goneau, Lee W. Janssen, Harry L.A. Mazzulli, Tony Cloherty, Gavin de Knegt, Robert J. Feld, Jordan J. Hepatitis C Core-Antigen Testing from Dried Blood Spots |
title | Hepatitis C Core-Antigen Testing from Dried Blood Spots |
title_full | Hepatitis C Core-Antigen Testing from Dried Blood Spots |
title_fullStr | Hepatitis C Core-Antigen Testing from Dried Blood Spots |
title_full_unstemmed | Hepatitis C Core-Antigen Testing from Dried Blood Spots |
title_short | Hepatitis C Core-Antigen Testing from Dried Blood Spots |
title_sort | hepatitis c core-antigen testing from dried blood spots |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784259/ https://www.ncbi.nlm.nih.gov/pubmed/31489933 http://dx.doi.org/10.3390/v11090830 |
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