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Bletilla striata polysaccharides ameliorates lipopolysaccharide-induced injury in intestinal epithelial cells

BACKGROUND/AIMS: This study was carried out to investigate the effect of Bletilla striata polysaccharide (BSP) treating on lipopolysaccharide (LPS)-induced intestinal epithelial barrier disruption in rat intestinal epithelial cell (IEC) line. MATERIALS AND METHODS: LPS was used to stimulate the IEC-...

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Autores principales: Luo, Lei, Liu, Yuqing, Cai, Xin, Wang, Yao, Xue, Juan, Zhang, Juan, Yang, Fan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784437/
https://www.ncbi.nlm.nih.gov/pubmed/31044747
http://dx.doi.org/10.4103/sjg.SJG_520_18
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author Luo, Lei
Liu, Yuqing
Cai, Xin
Wang, Yao
Xue, Juan
Zhang, Juan
Yang, Fan
author_facet Luo, Lei
Liu, Yuqing
Cai, Xin
Wang, Yao
Xue, Juan
Zhang, Juan
Yang, Fan
author_sort Luo, Lei
collection PubMed
description BACKGROUND/AIMS: This study was carried out to investigate the effect of Bletilla striata polysaccharide (BSP) treating on lipopolysaccharide (LPS)-induced intestinal epithelial barrier disruption in rat intestinal epithelial cell (IEC) line. MATERIALS AND METHODS: LPS was used to stimulate the IEC-18 cells (1 μg/ml), with or without different concentrations of BSP (25, 50 and 100 μg/ml) for 24 h. Transepithelial electrical resistance (TEER) was measured to detect the permeability of cells. Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the cell supernatant were detected with the enzyme-linked immunosorbent assay (ELISA). Real-time polymerase chain reaction (PCR) was employed to detect the mRNA levels of zonulae occludens (ZO)-1 and occludin. Western blot and immunofluorescence analysis were used for analyzing the expression level and the distribution patterns of ZO-1 and occludin protein. RESULTS: After treatment with BSP, the IL-6 and TNF-α levels in the cell supernatant were significantly decreased compared with the experiment group (P < 0.05 or 0.01). The permeability of IEC was decreased in BSP groups when compared with the experiment group (P < 0.05 or 0.01). In addition, compared with the experiment group, treatment with BSP up-regulated mRNA and protein expression levels of ZO-1 and occludin, and kept the ZO-1 and occludin protein intact in IEC-18 cells injured with LPS (P < 0.05 or 0.01). CONCLUSION: BSP has the capacity to protect IEC-18 cells from LPS-induced injury. The mechanisms may be associated with decreasing the inflammatory cytokine levels of IL-6 and TNF-α, and elevating the expression of ZO-1 and occludin, which might serve as a new protective agent for LPS-induced intestinal epithelial barrier disruption.
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spelling pubmed-67844372019-10-16 Bletilla striata polysaccharides ameliorates lipopolysaccharide-induced injury in intestinal epithelial cells Luo, Lei Liu, Yuqing Cai, Xin Wang, Yao Xue, Juan Zhang, Juan Yang, Fan Saudi J Gastroenterol Original Article BACKGROUND/AIMS: This study was carried out to investigate the effect of Bletilla striata polysaccharide (BSP) treating on lipopolysaccharide (LPS)-induced intestinal epithelial barrier disruption in rat intestinal epithelial cell (IEC) line. MATERIALS AND METHODS: LPS was used to stimulate the IEC-18 cells (1 μg/ml), with or without different concentrations of BSP (25, 50 and 100 μg/ml) for 24 h. Transepithelial electrical resistance (TEER) was measured to detect the permeability of cells. Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the cell supernatant were detected with the enzyme-linked immunosorbent assay (ELISA). Real-time polymerase chain reaction (PCR) was employed to detect the mRNA levels of zonulae occludens (ZO)-1 and occludin. Western blot and immunofluorescence analysis were used for analyzing the expression level and the distribution patterns of ZO-1 and occludin protein. RESULTS: After treatment with BSP, the IL-6 and TNF-α levels in the cell supernatant were significantly decreased compared with the experiment group (P < 0.05 or 0.01). The permeability of IEC was decreased in BSP groups when compared with the experiment group (P < 0.05 or 0.01). In addition, compared with the experiment group, treatment with BSP up-regulated mRNA and protein expression levels of ZO-1 and occludin, and kept the ZO-1 and occludin protein intact in IEC-18 cells injured with LPS (P < 0.05 or 0.01). CONCLUSION: BSP has the capacity to protect IEC-18 cells from LPS-induced injury. The mechanisms may be associated with decreasing the inflammatory cytokine levels of IL-6 and TNF-α, and elevating the expression of ZO-1 and occludin, which might serve as a new protective agent for LPS-induced intestinal epithelial barrier disruption. Wolters Kluwer - Medknow 2019-09-26 /pmc/articles/PMC6784437/ /pubmed/31044747 http://dx.doi.org/10.4103/sjg.SJG_520_18 Text en Copyright: © 2019 Saudi Journal of Gastroenterology http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Luo, Lei
Liu, Yuqing
Cai, Xin
Wang, Yao
Xue, Juan
Zhang, Juan
Yang, Fan
Bletilla striata polysaccharides ameliorates lipopolysaccharide-induced injury in intestinal epithelial cells
title Bletilla striata polysaccharides ameliorates lipopolysaccharide-induced injury in intestinal epithelial cells
title_full Bletilla striata polysaccharides ameliorates lipopolysaccharide-induced injury in intestinal epithelial cells
title_fullStr Bletilla striata polysaccharides ameliorates lipopolysaccharide-induced injury in intestinal epithelial cells
title_full_unstemmed Bletilla striata polysaccharides ameliorates lipopolysaccharide-induced injury in intestinal epithelial cells
title_short Bletilla striata polysaccharides ameliorates lipopolysaccharide-induced injury in intestinal epithelial cells
title_sort bletilla striata polysaccharides ameliorates lipopolysaccharide-induced injury in intestinal epithelial cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784437/
https://www.ncbi.nlm.nih.gov/pubmed/31044747
http://dx.doi.org/10.4103/sjg.SJG_520_18
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