Cargando…

Chemical, Clinical and Histomorphometric Comparison between Equine Bone Manufactured through Enzymatic Antigen-Elimination and Bovine Bone Made Non-Antigenic Using a High-Temperature Process in Post-Extractive Socket Grafting. A Comparative Retrospective Clinical Study

Enzyme-deantigenic equine bone (EDEB) and anorganic bovine bone (ABB) are two xenografts made non-antigenic through different processing methods. This study aimed to characterize them for the presence of native bone collagen and other proteins and to compare their histomorphometric outcome when they...

Descripción completa

Detalles Bibliográficos
Autores principales: Di Stefano, Danilo Alessio, Zaniol, Terry, Cinci, Lorenzo, Pieri, Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784468/
https://www.ncbi.nlm.nih.gov/pubmed/31266159
http://dx.doi.org/10.3390/dj7030070
_version_ 1783457764356390912
author Di Stefano, Danilo Alessio
Zaniol, Terry
Cinci, Lorenzo
Pieri, Laura
author_facet Di Stefano, Danilo Alessio
Zaniol, Terry
Cinci, Lorenzo
Pieri, Laura
author_sort Di Stefano, Danilo Alessio
collection PubMed
description Enzyme-deantigenic equine bone (EDEB) and anorganic bovine bone (ABB) are two xenografts made non-antigenic through different processing methods. This study aimed to characterize them for the presence of native bone collagen and other proteins and to compare their histomorphometric outcome when they were used to graft post-extractive sockets. The records of 46 patients treated with EDEB (n = 22) or ABB (n = 24) and followed-up for at least four months after delayed implant placement, were retrospectively collected. Samples of EDEB and ABB were analyzed using Attenuated Total Reflection Fourier Transform Infrared and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis for the presence of collagen and other proteins. For histomorphometric analysis on bone specimens, newly formed bone and residual biomaterial percentages were calculated. Results of the present study show that EDEB contains type I bone collagen in its native conformation, while no proteins were detected in ABB. Grafting EDEB resulted in a significantly greater quantity of newly formed bone and less residual biomaterial. Our findings suggest that the manufacturing process can greatly affect the graft behavior and a process preserving collagen in its native form may favor bone tissue regeneration.
format Online
Article
Text
id pubmed-6784468
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-67844682019-10-16 Chemical, Clinical and Histomorphometric Comparison between Equine Bone Manufactured through Enzymatic Antigen-Elimination and Bovine Bone Made Non-Antigenic Using a High-Temperature Process in Post-Extractive Socket Grafting. A Comparative Retrospective Clinical Study Di Stefano, Danilo Alessio Zaniol, Terry Cinci, Lorenzo Pieri, Laura Dent J (Basel) Article Enzyme-deantigenic equine bone (EDEB) and anorganic bovine bone (ABB) are two xenografts made non-antigenic through different processing methods. This study aimed to characterize them for the presence of native bone collagen and other proteins and to compare their histomorphometric outcome when they were used to graft post-extractive sockets. The records of 46 patients treated with EDEB (n = 22) or ABB (n = 24) and followed-up for at least four months after delayed implant placement, were retrospectively collected. Samples of EDEB and ABB were analyzed using Attenuated Total Reflection Fourier Transform Infrared and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis for the presence of collagen and other proteins. For histomorphometric analysis on bone specimens, newly formed bone and residual biomaterial percentages were calculated. Results of the present study show that EDEB contains type I bone collagen in its native conformation, while no proteins were detected in ABB. Grafting EDEB resulted in a significantly greater quantity of newly formed bone and less residual biomaterial. Our findings suggest that the manufacturing process can greatly affect the graft behavior and a process preserving collagen in its native form may favor bone tissue regeneration. MDPI 2019-07-01 /pmc/articles/PMC6784468/ /pubmed/31266159 http://dx.doi.org/10.3390/dj7030070 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Di Stefano, Danilo Alessio
Zaniol, Terry
Cinci, Lorenzo
Pieri, Laura
Chemical, Clinical and Histomorphometric Comparison between Equine Bone Manufactured through Enzymatic Antigen-Elimination and Bovine Bone Made Non-Antigenic Using a High-Temperature Process in Post-Extractive Socket Grafting. A Comparative Retrospective Clinical Study
title Chemical, Clinical and Histomorphometric Comparison between Equine Bone Manufactured through Enzymatic Antigen-Elimination and Bovine Bone Made Non-Antigenic Using a High-Temperature Process in Post-Extractive Socket Grafting. A Comparative Retrospective Clinical Study
title_full Chemical, Clinical and Histomorphometric Comparison between Equine Bone Manufactured through Enzymatic Antigen-Elimination and Bovine Bone Made Non-Antigenic Using a High-Temperature Process in Post-Extractive Socket Grafting. A Comparative Retrospective Clinical Study
title_fullStr Chemical, Clinical and Histomorphometric Comparison between Equine Bone Manufactured through Enzymatic Antigen-Elimination and Bovine Bone Made Non-Antigenic Using a High-Temperature Process in Post-Extractive Socket Grafting. A Comparative Retrospective Clinical Study
title_full_unstemmed Chemical, Clinical and Histomorphometric Comparison between Equine Bone Manufactured through Enzymatic Antigen-Elimination and Bovine Bone Made Non-Antigenic Using a High-Temperature Process in Post-Extractive Socket Grafting. A Comparative Retrospective Clinical Study
title_short Chemical, Clinical and Histomorphometric Comparison between Equine Bone Manufactured through Enzymatic Antigen-Elimination and Bovine Bone Made Non-Antigenic Using a High-Temperature Process in Post-Extractive Socket Grafting. A Comparative Retrospective Clinical Study
title_sort chemical, clinical and histomorphometric comparison between equine bone manufactured through enzymatic antigen-elimination and bovine bone made non-antigenic using a high-temperature process in post-extractive socket grafting. a comparative retrospective clinical study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784468/
https://www.ncbi.nlm.nih.gov/pubmed/31266159
http://dx.doi.org/10.3390/dj7030070
work_keys_str_mv AT distefanodaniloalessio chemicalclinicalandhistomorphometriccomparisonbetweenequinebonemanufacturedthroughenzymaticantigeneliminationandbovinebonemadenonantigenicusingahightemperatureprocessinpostextractivesocketgraftingacomparativeretrospectiveclinicalstudy
AT zaniolterry chemicalclinicalandhistomorphometriccomparisonbetweenequinebonemanufacturedthroughenzymaticantigeneliminationandbovinebonemadenonantigenicusingahightemperatureprocessinpostextractivesocketgraftingacomparativeretrospectiveclinicalstudy
AT cincilorenzo chemicalclinicalandhistomorphometriccomparisonbetweenequinebonemanufacturedthroughenzymaticantigeneliminationandbovinebonemadenonantigenicusingahightemperatureprocessinpostextractivesocketgraftingacomparativeretrospectiveclinicalstudy
AT pierilaura chemicalclinicalandhistomorphometriccomparisonbetweenequinebonemanufacturedthroughenzymaticantigeneliminationandbovinebonemadenonantigenicusingahightemperatureprocessinpostextractivesocketgraftingacomparativeretrospectiveclinicalstudy