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Draft genome assembly and transcriptome sequencing of the golden algae Hydrurus foetidus (Chrysophyceae)

Hydrurus foetidus is a freshwater chrysophyte alga. It thrives in cold rivers in polar and high alpine regions. It has several morphological traits reminiscent of single-celled eukaryotes, but can also form macroscopic thalli. Despite its ability to produce polyunsaturated fatty acids, its life unde...

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Autores principales: Bråte, Jon, Fuss, Janina, Mehrotra, Shruti, Jakobsen, Kjetill S., Klaveness, Dag
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784874/
https://www.ncbi.nlm.nih.gov/pubmed/31632652
http://dx.doi.org/10.12688/f1000research.16734.3
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author Bråte, Jon
Fuss, Janina
Mehrotra, Shruti
Jakobsen, Kjetill S.
Klaveness, Dag
author_facet Bråte, Jon
Fuss, Janina
Mehrotra, Shruti
Jakobsen, Kjetill S.
Klaveness, Dag
author_sort Bråte, Jon
collection PubMed
description Hydrurus foetidus is a freshwater chrysophyte alga. It thrives in cold rivers in polar and high alpine regions. It has several morphological traits reminiscent of single-celled eukaryotes, but can also form macroscopic thalli. Despite its ability to produce polyunsaturated fatty acids, its life under cold conditions and its variable morphology, very little is known about its genome and transcriptome. Here, we present an extensive set of next-generation sequencing data, including genomic short reads from Illumina sequencing and long reads from Nanopore sequencing, as well as full length cDNAs from PacBio IsoSeq sequencing and a small RNA dataset (smaller than 200 bp) sequenced with Illumina. The genome sequences were combined  to produce an assembly consisting of 5069 contigs, with a total assembly size of 171 Mb and a 77% BUSCO completeness. The new data generated here may contribute to a better understanding of the evolution and ecological roles of chrysophyte algae, as well as to resolve the branching patterns at a larger phylogenetic scale.
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spelling pubmed-67848742019-10-17 Draft genome assembly and transcriptome sequencing of the golden algae Hydrurus foetidus (Chrysophyceae) Bråte, Jon Fuss, Janina Mehrotra, Shruti Jakobsen, Kjetill S. Klaveness, Dag F1000Res Data Note Hydrurus foetidus is a freshwater chrysophyte alga. It thrives in cold rivers in polar and high alpine regions. It has several morphological traits reminiscent of single-celled eukaryotes, but can also form macroscopic thalli. Despite its ability to produce polyunsaturated fatty acids, its life under cold conditions and its variable morphology, very little is known about its genome and transcriptome. Here, we present an extensive set of next-generation sequencing data, including genomic short reads from Illumina sequencing and long reads from Nanopore sequencing, as well as full length cDNAs from PacBio IsoSeq sequencing and a small RNA dataset (smaller than 200 bp) sequenced with Illumina. The genome sequences were combined  to produce an assembly consisting of 5069 contigs, with a total assembly size of 171 Mb and a 77% BUSCO completeness. The new data generated here may contribute to a better understanding of the evolution and ecological roles of chrysophyte algae, as well as to resolve the branching patterns at a larger phylogenetic scale. F1000 Research Limited 2019-10-07 /pmc/articles/PMC6784874/ /pubmed/31632652 http://dx.doi.org/10.12688/f1000research.16734.3 Text en Copyright: © 2019 Bråte J et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Data Note
Bråte, Jon
Fuss, Janina
Mehrotra, Shruti
Jakobsen, Kjetill S.
Klaveness, Dag
Draft genome assembly and transcriptome sequencing of the golden algae Hydrurus foetidus (Chrysophyceae)
title Draft genome assembly and transcriptome sequencing of the golden algae Hydrurus foetidus (Chrysophyceae)
title_full Draft genome assembly and transcriptome sequencing of the golden algae Hydrurus foetidus (Chrysophyceae)
title_fullStr Draft genome assembly and transcriptome sequencing of the golden algae Hydrurus foetidus (Chrysophyceae)
title_full_unstemmed Draft genome assembly and transcriptome sequencing of the golden algae Hydrurus foetidus (Chrysophyceae)
title_short Draft genome assembly and transcriptome sequencing of the golden algae Hydrurus foetidus (Chrysophyceae)
title_sort draft genome assembly and transcriptome sequencing of the golden algae hydrurus foetidus (chrysophyceae)
topic Data Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784874/
https://www.ncbi.nlm.nih.gov/pubmed/31632652
http://dx.doi.org/10.12688/f1000research.16734.3
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