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Draft genome assembly and transcriptome sequencing of the golden algae Hydrurus foetidus (Chrysophyceae)
Hydrurus foetidus is a freshwater chrysophyte alga. It thrives in cold rivers in polar and high alpine regions. It has several morphological traits reminiscent of single-celled eukaryotes, but can also form macroscopic thalli. Despite its ability to produce polyunsaturated fatty acids, its life unde...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
F1000 Research Limited
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784874/ https://www.ncbi.nlm.nih.gov/pubmed/31632652 http://dx.doi.org/10.12688/f1000research.16734.3 |
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author | Bråte, Jon Fuss, Janina Mehrotra, Shruti Jakobsen, Kjetill S. Klaveness, Dag |
author_facet | Bråte, Jon Fuss, Janina Mehrotra, Shruti Jakobsen, Kjetill S. Klaveness, Dag |
author_sort | Bråte, Jon |
collection | PubMed |
description | Hydrurus foetidus is a freshwater chrysophyte alga. It thrives in cold rivers in polar and high alpine regions. It has several morphological traits reminiscent of single-celled eukaryotes, but can also form macroscopic thalli. Despite its ability to produce polyunsaturated fatty acids, its life under cold conditions and its variable morphology, very little is known about its genome and transcriptome. Here, we present an extensive set of next-generation sequencing data, including genomic short reads from Illumina sequencing and long reads from Nanopore sequencing, as well as full length cDNAs from PacBio IsoSeq sequencing and a small RNA dataset (smaller than 200 bp) sequenced with Illumina. The genome sequences were combined to produce an assembly consisting of 5069 contigs, with a total assembly size of 171 Mb and a 77% BUSCO completeness. The new data generated here may contribute to a better understanding of the evolution and ecological roles of chrysophyte algae, as well as to resolve the branching patterns at a larger phylogenetic scale. |
format | Online Article Text |
id | pubmed-6784874 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | F1000 Research Limited |
record_format | MEDLINE/PubMed |
spelling | pubmed-67848742019-10-17 Draft genome assembly and transcriptome sequencing of the golden algae Hydrurus foetidus (Chrysophyceae) Bråte, Jon Fuss, Janina Mehrotra, Shruti Jakobsen, Kjetill S. Klaveness, Dag F1000Res Data Note Hydrurus foetidus is a freshwater chrysophyte alga. It thrives in cold rivers in polar and high alpine regions. It has several morphological traits reminiscent of single-celled eukaryotes, but can also form macroscopic thalli. Despite its ability to produce polyunsaturated fatty acids, its life under cold conditions and its variable morphology, very little is known about its genome and transcriptome. Here, we present an extensive set of next-generation sequencing data, including genomic short reads from Illumina sequencing and long reads from Nanopore sequencing, as well as full length cDNAs from PacBio IsoSeq sequencing and a small RNA dataset (smaller than 200 bp) sequenced with Illumina. The genome sequences were combined to produce an assembly consisting of 5069 contigs, with a total assembly size of 171 Mb and a 77% BUSCO completeness. The new data generated here may contribute to a better understanding of the evolution and ecological roles of chrysophyte algae, as well as to resolve the branching patterns at a larger phylogenetic scale. F1000 Research Limited 2019-10-07 /pmc/articles/PMC6784874/ /pubmed/31632652 http://dx.doi.org/10.12688/f1000research.16734.3 Text en Copyright: © 2019 Bråte J et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Data Note Bråte, Jon Fuss, Janina Mehrotra, Shruti Jakobsen, Kjetill S. Klaveness, Dag Draft genome assembly and transcriptome sequencing of the golden algae Hydrurus foetidus (Chrysophyceae) |
title | Draft genome assembly and transcriptome sequencing of the golden algae
Hydrurus foetidus (Chrysophyceae) |
title_full | Draft genome assembly and transcriptome sequencing of the golden algae
Hydrurus foetidus (Chrysophyceae) |
title_fullStr | Draft genome assembly and transcriptome sequencing of the golden algae
Hydrurus foetidus (Chrysophyceae) |
title_full_unstemmed | Draft genome assembly and transcriptome sequencing of the golden algae
Hydrurus foetidus (Chrysophyceae) |
title_short | Draft genome assembly and transcriptome sequencing of the golden algae
Hydrurus foetidus (Chrysophyceae) |
title_sort | draft genome assembly and transcriptome sequencing of the golden algae
hydrurus foetidus (chrysophyceae) |
topic | Data Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784874/ https://www.ncbi.nlm.nih.gov/pubmed/31632652 http://dx.doi.org/10.12688/f1000research.16734.3 |
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