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Antibacterial properties and regenerative potential of Sr(2+) and Ce(3+) doped fluorapatites; a potential solution for peri-implantitis

Scaffolds and implants in orthopaedics and regenerative dentistry usually fail because of bacterial infections. A promising solution would be the development of biomaterials with both significant regenerative potential and enhanced antibacterial activity. Working towards this direction, fluorapatite...

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Autores principales: Anastasiou, A. D., Nerantzaki, M., Gounari, E., Duggal, M. S., Giannoudis, P. V., Jha, A., Bikiaris, D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6785562/
https://www.ncbi.nlm.nih.gov/pubmed/31597949
http://dx.doi.org/10.1038/s41598-019-50916-4
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author Anastasiou, A. D.
Nerantzaki, M.
Gounari, E.
Duggal, M. S.
Giannoudis, P. V.
Jha, A.
Bikiaris, D.
author_facet Anastasiou, A. D.
Nerantzaki, M.
Gounari, E.
Duggal, M. S.
Giannoudis, P. V.
Jha, A.
Bikiaris, D.
author_sort Anastasiou, A. D.
collection PubMed
description Scaffolds and implants in orthopaedics and regenerative dentistry usually fail because of bacterial infections. A promising solution would be the development of biomaterials with both significant regenerative potential and enhanced antibacterial activity. Working towards this direction, fluorapatite was synthesised and doped with Sr(2+) and Ce(3+) ions in order to tailor its properties. After experiments with four common bacteria (i.e. E. Coli, S. Aureus, B. Subtilis, B. Cereus), it was found that the undoped and the Ce(3+) doped fluorapatites present better antibacterial response than the Sr(2+) doped material. The synthesised minerals were incorporated into chitosan scaffolds and tested with Dental Pulp Stem Cells (DPSCs) to check their regenerative potential. As was expected, the scaffolds containing Sr(2+)-doped fluorapatite, presented high osteoconductivity leading to the differentiation of the DPSCs into osteoblasts. Similar results were obtained for the Ce(3+)-doped material, since both the concentration of osteocalcin and the RUNX2 gene expression were considerably higher than that for the un-doped mineral. Overall, it was shown that doping with Ce(3+) retains the good antibacterial profile of fluorapatite and enhances its regenerative potential, which makes it a promising option for dealing with conditions where healing of hard tissues is compromised by bacterial contamination.
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spelling pubmed-67855622019-10-17 Antibacterial properties and regenerative potential of Sr(2+) and Ce(3+) doped fluorapatites; a potential solution for peri-implantitis Anastasiou, A. D. Nerantzaki, M. Gounari, E. Duggal, M. S. Giannoudis, P. V. Jha, A. Bikiaris, D. Sci Rep Article Scaffolds and implants in orthopaedics and regenerative dentistry usually fail because of bacterial infections. A promising solution would be the development of biomaterials with both significant regenerative potential and enhanced antibacterial activity. Working towards this direction, fluorapatite was synthesised and doped with Sr(2+) and Ce(3+) ions in order to tailor its properties. After experiments with four common bacteria (i.e. E. Coli, S. Aureus, B. Subtilis, B. Cereus), it was found that the undoped and the Ce(3+) doped fluorapatites present better antibacterial response than the Sr(2+) doped material. The synthesised minerals were incorporated into chitosan scaffolds and tested with Dental Pulp Stem Cells (DPSCs) to check their regenerative potential. As was expected, the scaffolds containing Sr(2+)-doped fluorapatite, presented high osteoconductivity leading to the differentiation of the DPSCs into osteoblasts. Similar results were obtained for the Ce(3+)-doped material, since both the concentration of osteocalcin and the RUNX2 gene expression were considerably higher than that for the un-doped mineral. Overall, it was shown that doping with Ce(3+) retains the good antibacterial profile of fluorapatite and enhances its regenerative potential, which makes it a promising option for dealing with conditions where healing of hard tissues is compromised by bacterial contamination. Nature Publishing Group UK 2019-10-09 /pmc/articles/PMC6785562/ /pubmed/31597949 http://dx.doi.org/10.1038/s41598-019-50916-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Anastasiou, A. D.
Nerantzaki, M.
Gounari, E.
Duggal, M. S.
Giannoudis, P. V.
Jha, A.
Bikiaris, D.
Antibacterial properties and regenerative potential of Sr(2+) and Ce(3+) doped fluorapatites; a potential solution for peri-implantitis
title Antibacterial properties and regenerative potential of Sr(2+) and Ce(3+) doped fluorapatites; a potential solution for peri-implantitis
title_full Antibacterial properties and regenerative potential of Sr(2+) and Ce(3+) doped fluorapatites; a potential solution for peri-implantitis
title_fullStr Antibacterial properties and regenerative potential of Sr(2+) and Ce(3+) doped fluorapatites; a potential solution for peri-implantitis
title_full_unstemmed Antibacterial properties and regenerative potential of Sr(2+) and Ce(3+) doped fluorapatites; a potential solution for peri-implantitis
title_short Antibacterial properties and regenerative potential of Sr(2+) and Ce(3+) doped fluorapatites; a potential solution for peri-implantitis
title_sort antibacterial properties and regenerative potential of sr(2+) and ce(3+) doped fluorapatites; a potential solution for peri-implantitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6785562/
https://www.ncbi.nlm.nih.gov/pubmed/31597949
http://dx.doi.org/10.1038/s41598-019-50916-4
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