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Selection of Bacterial Mutants in Late Infections: When Vector Transmission Trades Off against Growth Advantage in Stationary Phase

Bacterial infections are often composed of cells with distinct phenotypes that can be produced by genetic or epigenetic mechanisms. This phenotypic heterogeneity has proved to be important in many pathogens, because it can alter both pathogenicity and transmission. We studied how and why it can emer...

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Autores principales: Cambon, Marine C., Parthuisot, Nathalie, Pagès, Sylvie, Lanois, Anne, Givaudan, Alain, Ferdy, Jean-Baptiste
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6786866/
https://www.ncbi.nlm.nih.gov/pubmed/31594811
http://dx.doi.org/10.1128/mBio.01437-19
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author Cambon, Marine C.
Parthuisot, Nathalie
Pagès, Sylvie
Lanois, Anne
Givaudan, Alain
Ferdy, Jean-Baptiste
author_facet Cambon, Marine C.
Parthuisot, Nathalie
Pagès, Sylvie
Lanois, Anne
Givaudan, Alain
Ferdy, Jean-Baptiste
author_sort Cambon, Marine C.
collection PubMed
description Bacterial infections are often composed of cells with distinct phenotypes that can be produced by genetic or epigenetic mechanisms. This phenotypic heterogeneity has proved to be important in many pathogens, because it can alter both pathogenicity and transmission. We studied how and why it can emerge during infection in the bacterium Xenorhabdus nematophila, a pathogen that kills insects and multiplies in the cadaver before being transmitted by the soil nematode vector Steinernema carpocapsae. We found that phenotypic variants cluster in three groups, one of which is composed of lrp defective mutants. These mutants, together with variants of another group, have in common that they maintain high survival during late stationary phase. This probably explains why they increase in frequency: variants of X. nematophila with a growth advantage in stationary phase (GASP) are under strong positive selection both in prolonged culture and in late infections. We also found that the within-host advantage of these variants seems to trade off against transmission by nematode vectors: the variants that reach the highest load in insects are those that are the least transmitted.
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spelling pubmed-67868662019-10-15 Selection of Bacterial Mutants in Late Infections: When Vector Transmission Trades Off against Growth Advantage in Stationary Phase Cambon, Marine C. Parthuisot, Nathalie Pagès, Sylvie Lanois, Anne Givaudan, Alain Ferdy, Jean-Baptiste mBio Research Article Bacterial infections are often composed of cells with distinct phenotypes that can be produced by genetic or epigenetic mechanisms. This phenotypic heterogeneity has proved to be important in many pathogens, because it can alter both pathogenicity and transmission. We studied how and why it can emerge during infection in the bacterium Xenorhabdus nematophila, a pathogen that kills insects and multiplies in the cadaver before being transmitted by the soil nematode vector Steinernema carpocapsae. We found that phenotypic variants cluster in three groups, one of which is composed of lrp defective mutants. These mutants, together with variants of another group, have in common that they maintain high survival during late stationary phase. This probably explains why they increase in frequency: variants of X. nematophila with a growth advantage in stationary phase (GASP) are under strong positive selection both in prolonged culture and in late infections. We also found that the within-host advantage of these variants seems to trade off against transmission by nematode vectors: the variants that reach the highest load in insects are those that are the least transmitted. American Society for Microbiology 2019-10-08 /pmc/articles/PMC6786866/ /pubmed/31594811 http://dx.doi.org/10.1128/mBio.01437-19 Text en Copyright © 2019 Cambon et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Cambon, Marine C.
Parthuisot, Nathalie
Pagès, Sylvie
Lanois, Anne
Givaudan, Alain
Ferdy, Jean-Baptiste
Selection of Bacterial Mutants in Late Infections: When Vector Transmission Trades Off against Growth Advantage in Stationary Phase
title Selection of Bacterial Mutants in Late Infections: When Vector Transmission Trades Off against Growth Advantage in Stationary Phase
title_full Selection of Bacterial Mutants in Late Infections: When Vector Transmission Trades Off against Growth Advantage in Stationary Phase
title_fullStr Selection of Bacterial Mutants in Late Infections: When Vector Transmission Trades Off against Growth Advantage in Stationary Phase
title_full_unstemmed Selection of Bacterial Mutants in Late Infections: When Vector Transmission Trades Off against Growth Advantage in Stationary Phase
title_short Selection of Bacterial Mutants in Late Infections: When Vector Transmission Trades Off against Growth Advantage in Stationary Phase
title_sort selection of bacterial mutants in late infections: when vector transmission trades off against growth advantage in stationary phase
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6786866/
https://www.ncbi.nlm.nih.gov/pubmed/31594811
http://dx.doi.org/10.1128/mBio.01437-19
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