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Selection of Bacterial Mutants in Late Infections: When Vector Transmission Trades Off against Growth Advantage in Stationary Phase
Bacterial infections are often composed of cells with distinct phenotypes that can be produced by genetic or epigenetic mechanisms. This phenotypic heterogeneity has proved to be important in many pathogens, because it can alter both pathogenicity and transmission. We studied how and why it can emer...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6786866/ https://www.ncbi.nlm.nih.gov/pubmed/31594811 http://dx.doi.org/10.1128/mBio.01437-19 |
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author | Cambon, Marine C. Parthuisot, Nathalie Pagès, Sylvie Lanois, Anne Givaudan, Alain Ferdy, Jean-Baptiste |
author_facet | Cambon, Marine C. Parthuisot, Nathalie Pagès, Sylvie Lanois, Anne Givaudan, Alain Ferdy, Jean-Baptiste |
author_sort | Cambon, Marine C. |
collection | PubMed |
description | Bacterial infections are often composed of cells with distinct phenotypes that can be produced by genetic or epigenetic mechanisms. This phenotypic heterogeneity has proved to be important in many pathogens, because it can alter both pathogenicity and transmission. We studied how and why it can emerge during infection in the bacterium Xenorhabdus nematophila, a pathogen that kills insects and multiplies in the cadaver before being transmitted by the soil nematode vector Steinernema carpocapsae. We found that phenotypic variants cluster in three groups, one of which is composed of lrp defective mutants. These mutants, together with variants of another group, have in common that they maintain high survival during late stationary phase. This probably explains why they increase in frequency: variants of X. nematophila with a growth advantage in stationary phase (GASP) are under strong positive selection both in prolonged culture and in late infections. We also found that the within-host advantage of these variants seems to trade off against transmission by nematode vectors: the variants that reach the highest load in insects are those that are the least transmitted. |
format | Online Article Text |
id | pubmed-6786866 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-67868662019-10-15 Selection of Bacterial Mutants in Late Infections: When Vector Transmission Trades Off against Growth Advantage in Stationary Phase Cambon, Marine C. Parthuisot, Nathalie Pagès, Sylvie Lanois, Anne Givaudan, Alain Ferdy, Jean-Baptiste mBio Research Article Bacterial infections are often composed of cells with distinct phenotypes that can be produced by genetic or epigenetic mechanisms. This phenotypic heterogeneity has proved to be important in many pathogens, because it can alter both pathogenicity and transmission. We studied how and why it can emerge during infection in the bacterium Xenorhabdus nematophila, a pathogen that kills insects and multiplies in the cadaver before being transmitted by the soil nematode vector Steinernema carpocapsae. We found that phenotypic variants cluster in three groups, one of which is composed of lrp defective mutants. These mutants, together with variants of another group, have in common that they maintain high survival during late stationary phase. This probably explains why they increase in frequency: variants of X. nematophila with a growth advantage in stationary phase (GASP) are under strong positive selection both in prolonged culture and in late infections. We also found that the within-host advantage of these variants seems to trade off against transmission by nematode vectors: the variants that reach the highest load in insects are those that are the least transmitted. American Society for Microbiology 2019-10-08 /pmc/articles/PMC6786866/ /pubmed/31594811 http://dx.doi.org/10.1128/mBio.01437-19 Text en Copyright © 2019 Cambon et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Cambon, Marine C. Parthuisot, Nathalie Pagès, Sylvie Lanois, Anne Givaudan, Alain Ferdy, Jean-Baptiste Selection of Bacterial Mutants in Late Infections: When Vector Transmission Trades Off against Growth Advantage in Stationary Phase |
title | Selection of Bacterial Mutants in Late Infections: When Vector Transmission Trades Off against Growth Advantage in Stationary Phase |
title_full | Selection of Bacterial Mutants in Late Infections: When Vector Transmission Trades Off against Growth Advantage in Stationary Phase |
title_fullStr | Selection of Bacterial Mutants in Late Infections: When Vector Transmission Trades Off against Growth Advantage in Stationary Phase |
title_full_unstemmed | Selection of Bacterial Mutants in Late Infections: When Vector Transmission Trades Off against Growth Advantage in Stationary Phase |
title_short | Selection of Bacterial Mutants in Late Infections: When Vector Transmission Trades Off against Growth Advantage in Stationary Phase |
title_sort | selection of bacterial mutants in late infections: when vector transmission trades off against growth advantage in stationary phase |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6786866/ https://www.ncbi.nlm.nih.gov/pubmed/31594811 http://dx.doi.org/10.1128/mBio.01437-19 |
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