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Meiotic crossovers characterized by haplotype-specific chromosome painting in maize

Meiotic crossovers (COs) play a critical role in generating genetic variation and maintaining faithful segregation of homologous chromosomes during meiosis. We develop a haplotype-specific fluorescence in situ hybridization (FISH) technique that allows visualization of COs directly on metaphase chro...

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Autores principales: do Vale Martins, Lívia, Yu, Fan, Zhao, Hainan, Dennison, Tesia, Lauter, Nick, Wang, Haiyan, Deng, Zuhu, Thompson, Addie, Semrau, Kassandra, Rouillard, Jean-Marie, Birchler, James A., Jiang, Jiming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6787048/
https://www.ncbi.nlm.nih.gov/pubmed/31601818
http://dx.doi.org/10.1038/s41467-019-12646-z
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author do Vale Martins, Lívia
Yu, Fan
Zhao, Hainan
Dennison, Tesia
Lauter, Nick
Wang, Haiyan
Deng, Zuhu
Thompson, Addie
Semrau, Kassandra
Rouillard, Jean-Marie
Birchler, James A.
Jiang, Jiming
author_facet do Vale Martins, Lívia
Yu, Fan
Zhao, Hainan
Dennison, Tesia
Lauter, Nick
Wang, Haiyan
Deng, Zuhu
Thompson, Addie
Semrau, Kassandra
Rouillard, Jean-Marie
Birchler, James A.
Jiang, Jiming
author_sort do Vale Martins, Lívia
collection PubMed
description Meiotic crossovers (COs) play a critical role in generating genetic variation and maintaining faithful segregation of homologous chromosomes during meiosis. We develop a haplotype-specific fluorescence in situ hybridization (FISH) technique that allows visualization of COs directly on metaphase chromosomes. Oligonucleotides (oligos) specific to chromosome 10 of maize inbreds B73 and Mo17, respectively, are synthesized and labeled as FISH probes. The parental and recombinant chromosome 10 in B73 x Mo17 F(1) hybrids and F(2) progenies can be unambiguously identified by haplotype-specific FISH. Analysis of 58 F(2) plants reveals lack of COs in the entire proximal half of chromosome 10. However, we detect COs located in regions very close to the centromere in recombinant inbred lines from an intermated B73 x Mo17 population, suggesting effective accumulation of COs in recombination-suppressed chromosomal regions through intermating and the potential to generate favorable allelic combinations of genes residing in these regions.
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spelling pubmed-67870482019-10-15 Meiotic crossovers characterized by haplotype-specific chromosome painting in maize do Vale Martins, Lívia Yu, Fan Zhao, Hainan Dennison, Tesia Lauter, Nick Wang, Haiyan Deng, Zuhu Thompson, Addie Semrau, Kassandra Rouillard, Jean-Marie Birchler, James A. Jiang, Jiming Nat Commun Article Meiotic crossovers (COs) play a critical role in generating genetic variation and maintaining faithful segregation of homologous chromosomes during meiosis. We develop a haplotype-specific fluorescence in situ hybridization (FISH) technique that allows visualization of COs directly on metaphase chromosomes. Oligonucleotides (oligos) specific to chromosome 10 of maize inbreds B73 and Mo17, respectively, are synthesized and labeled as FISH probes. The parental and recombinant chromosome 10 in B73 x Mo17 F(1) hybrids and F(2) progenies can be unambiguously identified by haplotype-specific FISH. Analysis of 58 F(2) plants reveals lack of COs in the entire proximal half of chromosome 10. However, we detect COs located in regions very close to the centromere in recombinant inbred lines from an intermated B73 x Mo17 population, suggesting effective accumulation of COs in recombination-suppressed chromosomal regions through intermating and the potential to generate favorable allelic combinations of genes residing in these regions. Nature Publishing Group UK 2019-10-10 /pmc/articles/PMC6787048/ /pubmed/31601818 http://dx.doi.org/10.1038/s41467-019-12646-z Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
do Vale Martins, Lívia
Yu, Fan
Zhao, Hainan
Dennison, Tesia
Lauter, Nick
Wang, Haiyan
Deng, Zuhu
Thompson, Addie
Semrau, Kassandra
Rouillard, Jean-Marie
Birchler, James A.
Jiang, Jiming
Meiotic crossovers characterized by haplotype-specific chromosome painting in maize
title Meiotic crossovers characterized by haplotype-specific chromosome painting in maize
title_full Meiotic crossovers characterized by haplotype-specific chromosome painting in maize
title_fullStr Meiotic crossovers characterized by haplotype-specific chromosome painting in maize
title_full_unstemmed Meiotic crossovers characterized by haplotype-specific chromosome painting in maize
title_short Meiotic crossovers characterized by haplotype-specific chromosome painting in maize
title_sort meiotic crossovers characterized by haplotype-specific chromosome painting in maize
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6787048/
https://www.ncbi.nlm.nih.gov/pubmed/31601818
http://dx.doi.org/10.1038/s41467-019-12646-z
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