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A one-step tRNA-CRISPR system for genome-wide genetic interaction mapping in mammalian cells

Mapping genetic interactions in mammalian cells is limited due to technical obstacles. Here we describe a method called TCGI (tRNA-CRISPR for genetic interactions) to generate a high-efficient, barcode-free and scalable pairwise CRISPR libraries in mammalian cells for identifying genetic interaction...

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Detalles Bibliográficos
Autores principales:	Zhao, Yulei, Tyrishkin, Kathrin, Sjaarda, Calvin, Khanal, Prem, Stafford, Jeff, Rauh, Michael, Liu, Xudong, Babak, Tomas, Yang, Xiaolong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6787096/ https://www.ncbi.nlm.nih.gov/pubmed/31601883 http://dx.doi.org/10.1038/s41598-019-51090-3

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