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Establishment and Characterization of Two Novel Cholangiocarcinoma Cell Lines

BACKGROUND: Cholangiocarcinoma (CCA) is a rare, aggressive and highly lethal tumor. The disease is very difficult to diagnose and multi-modality treatments are ineffective. To improve our understanding of the biological characteristics of CCA, and facilitate the identification of valid treatments, a...

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Autores principales: Zhang, Yanhua, Luo, Jingfeng, Dong, Xue, Yang, Fang, Zhang, Miaofeng, Zhao, Juanjuan, Wang, Qiangfeng, Zhou, Fei, Sun, Jihong, Yang, Xiaoming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6787115/
https://www.ncbi.nlm.nih.gov/pubmed/31359275
http://dx.doi.org/10.1245/s10434-019-07649-5
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author Zhang, Yanhua
Luo, Jingfeng
Dong, Xue
Yang, Fang
Zhang, Miaofeng
Zhao, Juanjuan
Wang, Qiangfeng
Zhou, Fei
Sun, Jihong
Yang, Xiaoming
author_facet Zhang, Yanhua
Luo, Jingfeng
Dong, Xue
Yang, Fang
Zhang, Miaofeng
Zhao, Juanjuan
Wang, Qiangfeng
Zhou, Fei
Sun, Jihong
Yang, Xiaoming
author_sort Zhang, Yanhua
collection PubMed
description BACKGROUND: Cholangiocarcinoma (CCA) is a rare, aggressive and highly lethal tumor. The disease is very difficult to diagnose and multi-modality treatments are ineffective. To improve our understanding of the biological characteristics of CCA, and facilitate the identification of valid treatments, an in-depth characterization of two novel Chinese patient-derived primary CCA cell lines was performed. METHODS: Two CCA cell lines were developed and labelled ZJU-0826 and -1125. The two cell lines were characterized with respect to phenotypic, molecular, biomarker, functional and histological properties. RESULTS: Two novel cell lines were cultured for 2 years, and maintained for more than 100 passages. They retained their typical biliary epithelial morphology and ultrastructure. The population doubling times of ZJU-0826, and -1125 were 63.84 h and 44.73 h, respectively. The cells exhibited near-triploid karyotypes with complex structural aberrations. ZJU-1125 cells had mutations in TP53 exons. Short tandem repeats genotyping confirmed the human origin and difference between lines. An immunophenotype analysis showed that ZJU-0826 is positive for CD44, CD29, Pdx1, CD236, FoxA1, FoxA2, and Nanog, and ZJU-1125 positive for CD44, CD29, CD133, Pdx1, FoxA1, FoxA2, and Nanog. ZJU-1125 had greater invasion ability in vitro and tumorigenicity than those of ZJU-0826. CONCLUSIONS: Our results confirm the validity of the ZJU-0826 and -1125 as representative models for the elucidation of the molecular pathogenesis of perihilar CCA, and intrahepatic CCA in both in vitro and in vivo studies, respectively. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1245/s10434-019-07649-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-67871152019-10-17 Establishment and Characterization of Two Novel Cholangiocarcinoma Cell Lines Zhang, Yanhua Luo, Jingfeng Dong, Xue Yang, Fang Zhang, Miaofeng Zhao, Juanjuan Wang, Qiangfeng Zhou, Fei Sun, Jihong Yang, Xiaoming Ann Surg Oncol Translational Research and Biomarkers BACKGROUND: Cholangiocarcinoma (CCA) is a rare, aggressive and highly lethal tumor. The disease is very difficult to diagnose and multi-modality treatments are ineffective. To improve our understanding of the biological characteristics of CCA, and facilitate the identification of valid treatments, an in-depth characterization of two novel Chinese patient-derived primary CCA cell lines was performed. METHODS: Two CCA cell lines were developed and labelled ZJU-0826 and -1125. The two cell lines were characterized with respect to phenotypic, molecular, biomarker, functional and histological properties. RESULTS: Two novel cell lines were cultured for 2 years, and maintained for more than 100 passages. They retained their typical biliary epithelial morphology and ultrastructure. The population doubling times of ZJU-0826, and -1125 were 63.84 h and 44.73 h, respectively. The cells exhibited near-triploid karyotypes with complex structural aberrations. ZJU-1125 cells had mutations in TP53 exons. Short tandem repeats genotyping confirmed the human origin and difference between lines. An immunophenotype analysis showed that ZJU-0826 is positive for CD44, CD29, Pdx1, CD236, FoxA1, FoxA2, and Nanog, and ZJU-1125 positive for CD44, CD29, CD133, Pdx1, FoxA1, FoxA2, and Nanog. ZJU-1125 had greater invasion ability in vitro and tumorigenicity than those of ZJU-0826. CONCLUSIONS: Our results confirm the validity of the ZJU-0826 and -1125 as representative models for the elucidation of the molecular pathogenesis of perihilar CCA, and intrahepatic CCA in both in vitro and in vivo studies, respectively. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1245/s10434-019-07649-5) contains supplementary material, which is available to authorized users. Springer International Publishing 2019-07-29 2019 /pmc/articles/PMC6787115/ /pubmed/31359275 http://dx.doi.org/10.1245/s10434-019-07649-5 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Translational Research and Biomarkers
Zhang, Yanhua
Luo, Jingfeng
Dong, Xue
Yang, Fang
Zhang, Miaofeng
Zhao, Juanjuan
Wang, Qiangfeng
Zhou, Fei
Sun, Jihong
Yang, Xiaoming
Establishment and Characterization of Two Novel Cholangiocarcinoma Cell Lines
title Establishment and Characterization of Two Novel Cholangiocarcinoma Cell Lines
title_full Establishment and Characterization of Two Novel Cholangiocarcinoma Cell Lines
title_fullStr Establishment and Characterization of Two Novel Cholangiocarcinoma Cell Lines
title_full_unstemmed Establishment and Characterization of Two Novel Cholangiocarcinoma Cell Lines
title_short Establishment and Characterization of Two Novel Cholangiocarcinoma Cell Lines
title_sort establishment and characterization of two novel cholangiocarcinoma cell lines
topic Translational Research and Biomarkers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6787115/
https://www.ncbi.nlm.nih.gov/pubmed/31359275
http://dx.doi.org/10.1245/s10434-019-07649-5
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