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Surface Study of Fe(3)O(4) Nanoparticles Functionalized With Biocompatible Adsorbed Molecules

Surfaces of iron oxide of ferrimagnetic magnetite (Fe(3)O(4)) nanoparticles (MNPs) prepared by Massart's method and their functionalized form (f-MNPs) with succinic acid, L-arginine, oxalic acid, citric acid, and glutamic acid were studied by dynamic light scattering (DLS), Fourier transform in...

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Autores principales: Lesiak, Beata, Rangam, N., Jiricek, P., Gordeev, I., Tóth, J., Kövér, L., Mohai, M., Borowicz, P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6787174/
https://www.ncbi.nlm.nih.gov/pubmed/31637230
http://dx.doi.org/10.3389/fchem.2019.00642
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author Lesiak, Beata
Rangam, N.
Jiricek, P.
Gordeev, I.
Tóth, J.
Kövér, L.
Mohai, M.
Borowicz, P.
author_facet Lesiak, Beata
Rangam, N.
Jiricek, P.
Gordeev, I.
Tóth, J.
Kövér, L.
Mohai, M.
Borowicz, P.
author_sort Lesiak, Beata
collection PubMed
description Surfaces of iron oxide of ferrimagnetic magnetite (Fe(3)O(4)) nanoparticles (MNPs) prepared by Massart's method and their functionalized form (f-MNPs) with succinic acid, L-arginine, oxalic acid, citric acid, and glutamic acid were studied by dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR-S), UV-vis, thermogravimetric analysis (TGA)/differential scanning calorimetry (DSC), X-ray photoelectron spectroscopy (XPS), and reflection electron energy loss spectroscopy (REELS). The XPS analysis of elements and their chemical states at the surface of MNPs and f-MNPs revealed differences in chemical bonding of atoms, content of carbon–oxygen groups, iron oxide forms, iron oxide magnetic properties, adsorbed molecules, surface coverage, and overlayer thickness, whereas the Auger parameters (derived from XPS and Auger spectra) and elastic and inelastic scattering probabilities of electrons on atoms and valence band electrons (derived from REELS spectra) indicated modification of surface charge redistribution, electronic, and optical properties. These modified properties of f-MNPs influenced their biological properties. The surfaces biocompatible for L929 cells showed various cytotoxicity for HeLa cells (10.8–5.3% of cell death), the highest for MNPs functionalized with oxalic acid. The samples exhibiting the largest efficiency possessed smaller surface coverage and thickness of adsorbed molecules layers, the highest content of oxygen and carbon–oxygen functionalizing groups, the highest ratio of lattice O(2−) and OH(−) to C sp(2) hybridizations on MNP surface, the highest ratio of adsorbed O(−) and OH(−) to C sp(2) hybridizations on adsorbed molecule layers, the closest electronic and optical properties to Fe(3)O(4), and the lowest degree of admolecule polymerization. This high cytotoxicity was attributed to interaction of cells with a surface, where increased content of oxygen groups, adsorbed O(−), and OH(−) may play the role of additional adsorption and catalytic sites and a large content of adsorbed molecule layers of carboxylic groups facilitating Fenton reaction kinetics leading to cell damage.
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spelling pubmed-67871742019-10-21 Surface Study of Fe(3)O(4) Nanoparticles Functionalized With Biocompatible Adsorbed Molecules Lesiak, Beata Rangam, N. Jiricek, P. Gordeev, I. Tóth, J. Kövér, L. Mohai, M. Borowicz, P. Front Chem Chemistry Surfaces of iron oxide of ferrimagnetic magnetite (Fe(3)O(4)) nanoparticles (MNPs) prepared by Massart's method and their functionalized form (f-MNPs) with succinic acid, L-arginine, oxalic acid, citric acid, and glutamic acid were studied by dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR-S), UV-vis, thermogravimetric analysis (TGA)/differential scanning calorimetry (DSC), X-ray photoelectron spectroscopy (XPS), and reflection electron energy loss spectroscopy (REELS). The XPS analysis of elements and their chemical states at the surface of MNPs and f-MNPs revealed differences in chemical bonding of atoms, content of carbon–oxygen groups, iron oxide forms, iron oxide magnetic properties, adsorbed molecules, surface coverage, and overlayer thickness, whereas the Auger parameters (derived from XPS and Auger spectra) and elastic and inelastic scattering probabilities of electrons on atoms and valence band electrons (derived from REELS spectra) indicated modification of surface charge redistribution, electronic, and optical properties. These modified properties of f-MNPs influenced their biological properties. The surfaces biocompatible for L929 cells showed various cytotoxicity for HeLa cells (10.8–5.3% of cell death), the highest for MNPs functionalized with oxalic acid. The samples exhibiting the largest efficiency possessed smaller surface coverage and thickness of adsorbed molecules layers, the highest content of oxygen and carbon–oxygen functionalizing groups, the highest ratio of lattice O(2−) and OH(−) to C sp(2) hybridizations on MNP surface, the highest ratio of adsorbed O(−) and OH(−) to C sp(2) hybridizations on adsorbed molecule layers, the closest electronic and optical properties to Fe(3)O(4), and the lowest degree of admolecule polymerization. This high cytotoxicity was attributed to interaction of cells with a surface, where increased content of oxygen groups, adsorbed O(−), and OH(−) may play the role of additional adsorption and catalytic sites and a large content of adsorbed molecule layers of carboxylic groups facilitating Fenton reaction kinetics leading to cell damage. Frontiers Media S.A. 2019-10-04 /pmc/articles/PMC6787174/ /pubmed/31637230 http://dx.doi.org/10.3389/fchem.2019.00642 Text en Copyright © 2019 Lesiak, Rangam, Jiricek, Gordeev, Tóth, Kövér, Mohai and Borowicz. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Lesiak, Beata
Rangam, N.
Jiricek, P.
Gordeev, I.
Tóth, J.
Kövér, L.
Mohai, M.
Borowicz, P.
Surface Study of Fe(3)O(4) Nanoparticles Functionalized With Biocompatible Adsorbed Molecules
title Surface Study of Fe(3)O(4) Nanoparticles Functionalized With Biocompatible Adsorbed Molecules
title_full Surface Study of Fe(3)O(4) Nanoparticles Functionalized With Biocompatible Adsorbed Molecules
title_fullStr Surface Study of Fe(3)O(4) Nanoparticles Functionalized With Biocompatible Adsorbed Molecules
title_full_unstemmed Surface Study of Fe(3)O(4) Nanoparticles Functionalized With Biocompatible Adsorbed Molecules
title_short Surface Study of Fe(3)O(4) Nanoparticles Functionalized With Biocompatible Adsorbed Molecules
title_sort surface study of fe(3)o(4) nanoparticles functionalized with biocompatible adsorbed molecules
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6787174/
https://www.ncbi.nlm.nih.gov/pubmed/31637230
http://dx.doi.org/10.3389/fchem.2019.00642
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