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Simulated microgravity with floating environment promotes migration of non-small cell lung cancers
A migration of cancer is one of the most important factors affecting cancer therapy. Particularly, a cancer migration study in a microgravity environment has gained attention as a tool for developing cancer therapy. In this study, we evaluated the proliferation and migration of two types (adenocarci...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6787256/ https://www.ncbi.nlm.nih.gov/pubmed/31601869 http://dx.doi.org/10.1038/s41598-019-50736-6 |
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author | Ahn, Chi Bum Lee, Ji-Hyun Han, Dae Geun Kang, Hyun-Wook Lee, Sung-Ho Lee, Jae-Ik Son, Kuk Hui Lee, Jin Woo |
author_facet | Ahn, Chi Bum Lee, Ji-Hyun Han, Dae Geun Kang, Hyun-Wook Lee, Sung-Ho Lee, Jae-Ik Son, Kuk Hui Lee, Jin Woo |
author_sort | Ahn, Chi Bum |
collection | PubMed |
description | A migration of cancer is one of the most important factors affecting cancer therapy. Particularly, a cancer migration study in a microgravity environment has gained attention as a tool for developing cancer therapy. In this study, we evaluated the proliferation and migration of two types (adenocarcinoma A549, squamous cell carcinoma H1703) of non-small cell lung cancers (NSCLC) in a floating environment with microgravity. When we measured proliferation of two NSCLCs in the microgravity (MG) and ground-gravity (CONT), although initial cell adhesion in MG was low, a normalized proliferation rate of A549 in MG was higher than that in CONT. Wound healing results of A549 and H1703 showed rapid recovery in MG; particularly, the migration rate of A549 was faster than that of H1703 both the normal and low proliferating conditions. Gene expression results showed that the microgravity accelerated the migration of NSCLC. Both A549 and H1703 in MG highly expressed the migration-related genes MMP-2, MMP-9, TIMP-1, and TIMP-2 compared to CONT at 24 h. Furthermore, analysis of MMP-2 protein synthesis revealed weaker metastatic performance of H1703 than that of A549. Therefore, the simulated microgravity based cancer culture environment will be a potential for migration and metastasis studies of lung cancers. |
format | Online Article Text |
id | pubmed-6787256 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-67872562019-10-17 Simulated microgravity with floating environment promotes migration of non-small cell lung cancers Ahn, Chi Bum Lee, Ji-Hyun Han, Dae Geun Kang, Hyun-Wook Lee, Sung-Ho Lee, Jae-Ik Son, Kuk Hui Lee, Jin Woo Sci Rep Article A migration of cancer is one of the most important factors affecting cancer therapy. Particularly, a cancer migration study in a microgravity environment has gained attention as a tool for developing cancer therapy. In this study, we evaluated the proliferation and migration of two types (adenocarcinoma A549, squamous cell carcinoma H1703) of non-small cell lung cancers (NSCLC) in a floating environment with microgravity. When we measured proliferation of two NSCLCs in the microgravity (MG) and ground-gravity (CONT), although initial cell adhesion in MG was low, a normalized proliferation rate of A549 in MG was higher than that in CONT. Wound healing results of A549 and H1703 showed rapid recovery in MG; particularly, the migration rate of A549 was faster than that of H1703 both the normal and low proliferating conditions. Gene expression results showed that the microgravity accelerated the migration of NSCLC. Both A549 and H1703 in MG highly expressed the migration-related genes MMP-2, MMP-9, TIMP-1, and TIMP-2 compared to CONT at 24 h. Furthermore, analysis of MMP-2 protein synthesis revealed weaker metastatic performance of H1703 than that of A549. Therefore, the simulated microgravity based cancer culture environment will be a potential for migration and metastasis studies of lung cancers. Nature Publishing Group UK 2019-10-10 /pmc/articles/PMC6787256/ /pubmed/31601869 http://dx.doi.org/10.1038/s41598-019-50736-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Ahn, Chi Bum Lee, Ji-Hyun Han, Dae Geun Kang, Hyun-Wook Lee, Sung-Ho Lee, Jae-Ik Son, Kuk Hui Lee, Jin Woo Simulated microgravity with floating environment promotes migration of non-small cell lung cancers |
title | Simulated microgravity with floating environment promotes migration of non-small cell lung cancers |
title_full | Simulated microgravity with floating environment promotes migration of non-small cell lung cancers |
title_fullStr | Simulated microgravity with floating environment promotes migration of non-small cell lung cancers |
title_full_unstemmed | Simulated microgravity with floating environment promotes migration of non-small cell lung cancers |
title_short | Simulated microgravity with floating environment promotes migration of non-small cell lung cancers |
title_sort | simulated microgravity with floating environment promotes migration of non-small cell lung cancers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6787256/ https://www.ncbi.nlm.nih.gov/pubmed/31601869 http://dx.doi.org/10.1038/s41598-019-50736-6 |
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