A New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction Enzymes

A series of new Escherichia coli entry vectors (pIIS18-SapI, pIIS18-BsmBI, pIIS18-BsaI, pIIS18-BfuAI-1, and pIIS18-BfuAI-2) was constructed based on a modified pUC18 backbone, which carried newly designed multiple cloning sites, consisting of two facing type IIS enzyme cleavage sites and one blunt-e...

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Detalles Bibliográficos
Autores principales: Altaib, Hend, Ozaki, Yuka, Kozakai, Tomoya, Badr, Yassien, Nomura, Izumi, Suzuki, Tohru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2019
Materias:
Other Genetic Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6787310/
https://www.ncbi.nlm.nih.gov/pubmed/31601653
http://dx.doi.org/10.1128/MRA.00323-19
Descripción
Sumario:A series of new Escherichia coli entry vectors (pIIS18-SapI, pIIS18-BsmBI, pIIS18-BsaI, pIIS18-BfuAI-1, and pIIS18-BfuAI-2) was constructed based on a modified pUC18 backbone, which carried newly designed multiple cloning sites, consisting of two facing type IIS enzyme cleavage sites and one blunt-end enzyme cleavage site. These vectors are useful for seamless gene cloning.

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