A New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction Enzymes

A series of new Escherichia coli entry vectors (pIIS18-SapI, pIIS18-BsmBI, pIIS18-BsaI, pIIS18-BfuAI-1, and pIIS18-BfuAI-2) was constructed based on a modified pUC18 backbone, which carried newly designed multiple cloning sites, consisting of two facing type IIS enzyme cleavage sites and one blunt-e...

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Autores principales: Altaib, Hend, Ozaki, Yuka, Kozakai, Tomoya, Badr, Yassien, Nomura, Izumi, Suzuki, Tohru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2019
Materias:
Other Genetic Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6787310/
https://www.ncbi.nlm.nih.gov/pubmed/31601653
http://dx.doi.org/10.1128/MRA.00323-19
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author Altaib, Hend
Ozaki, Yuka
Kozakai, Tomoya
Badr, Yassien
Nomura, Izumi
Suzuki, Tohru
author_facet Altaib, Hend
Ozaki, Yuka
Kozakai, Tomoya
Badr, Yassien
Nomura, Izumi
Suzuki, Tohru
author_sort Altaib, Hend
collection PubMed
description A series of new Escherichia coli entry vectors (pIIS18-SapI, pIIS18-BsmBI, pIIS18-BsaI, pIIS18-BfuAI-1, and pIIS18-BfuAI-2) was constructed based on a modified pUC18 backbone, which carried newly designed multiple cloning sites, consisting of two facing type IIS enzyme cleavage sites and one blunt-end enzyme cleavage site. These vectors are useful for seamless gene cloning.
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spelling pubmed-67873102019-10-21 A New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction Enzymes Altaib, Hend Ozaki, Yuka Kozakai, Tomoya Badr, Yassien Nomura, Izumi Suzuki, Tohru Microbiol Resour Announc Other Genetic Resources A series of new Escherichia coli entry vectors (pIIS18-SapI, pIIS18-BsmBI, pIIS18-BsaI, pIIS18-BfuAI-1, and pIIS18-BfuAI-2) was constructed based on a modified pUC18 backbone, which carried newly designed multiple cloning sites, consisting of two facing type IIS enzyme cleavage sites and one blunt-end enzyme cleavage site. These vectors are useful for seamless gene cloning. American Society for Microbiology 2019-10-10 /pmc/articles/PMC6787310/ /pubmed/31601653 http://dx.doi.org/10.1128/MRA.00323-19 Text en Copyright © 2019 Altaib et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Other Genetic Resources
Altaib, Hend
Ozaki, Yuka
Kozakai, Tomoya
Badr, Yassien
Nomura, Izumi
Suzuki, Tohru
A New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction Enzymes
title A New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction Enzymes
title_full A New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction Enzymes
title_fullStr A New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction Enzymes
title_full_unstemmed A New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction Enzymes
title_short A New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction Enzymes
title_sort new escherichia coli entry vector series (piis18) for seamless gene cloning using type iis restriction enzymes
topic Other Genetic Resources
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6787310/
https://www.ncbi.nlm.nih.gov/pubmed/31601653
http://dx.doi.org/10.1128/MRA.00323-19
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