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A New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction Enzymes

A series of new Escherichia coli entry vectors (pIIS18-SapI, pIIS18-BsmBI, pIIS18-BsaI, pIIS18-BfuAI-1, and pIIS18-BfuAI-2) was constructed based on a modified pUC18 backbone, which carried newly designed multiple cloning sites, consisting of two facing type IIS enzyme cleavage sites and one blunt-e...

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Detalles Bibliográficos
Autores principales:	Altaib, Hend, Ozaki, Yuka, Kozakai, Tomoya, Badr, Yassien, Nomura, Izumi, Suzuki, Tohru
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2019
Materias:	Other Genetic Resources
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6787310/ https://www.ncbi.nlm.nih.gov/pubmed/31601653 http://dx.doi.org/10.1128/MRA.00323-19

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