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Purification, kinetic characterization, and site-directed mutagenesis of Methanothermobacter thermautotrophicus RFAP Synthase Produced in Escherichia coli

Methane-producing archaea are among a select group of microorganisms that utilize tetrahydromethanopterin (H(4)MPT) as a one-carbon carrier instead of tetrahydrofolate. In H(4)MPT biosynthesis, β-ribofuranosylaminobenzene 5′-phosphate (RFAP) synthase catalyzes the production of RFAP, CO(2), and pyro...

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Detalles Bibliográficos
Autores principales:	Bechard, Matthew E., Farahani, Payam, Greene, Dina, Pham, Anna, Orry, Andrew, Rasche, Madeline E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	AIMS Press 2019
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6787355/ https://www.ncbi.nlm.nih.gov/pubmed/31663056 http://dx.doi.org/10.3934/microbiol.2019.3.186

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