Full-Length Infectious Clones of Two New Isolates of Tomato Mosaic Virus Induce Distinct Symptoms Associated with Two Differential Amino Acid Residues in 128-kDa Protein

In 2017, two new tomato mosaic virus (ToMV) isolates were collected from greenhouses in Buyeo, Chungcheongnam-do, South Korea. Full-length cDNAs of the new ToMV isolates were cloned into dual cauliflower mosaic virus 35S and T7 promoter-driven vectors, sequenced and their pathogenicities investigate...

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Autores principales: Choi, Go-Woon, Oh, June-Pyo, Cho, In-Sook, Ju, Hye-Kyoung, Hu, Wen-Xing, Kim, Boram, Seo, Eun-Young, Park, Jong-Seok, Domier, Leslie L, Hammond, John, Song, Kihak, Lim, Hyoun-Sub
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Plant Pathology 2019
Materias:
Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6788407/
https://www.ncbi.nlm.nih.gov/pubmed/31632228
http://dx.doi.org/10.5423/PPJ.NT.12.2018.0286
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author Choi, Go-Woon
Oh, June-Pyo
Cho, In-Sook
Ju, Hye-Kyoung
Hu, Wen-Xing
Kim, Boram
Seo, Eun-Young
Park, Jong-Seok
Domier, Leslie L
Hammond, John
Song, Kihak
Lim, Hyoun-Sub
author_facet Choi, Go-Woon
Oh, June-Pyo
Cho, In-Sook
Ju, Hye-Kyoung
Hu, Wen-Xing
Kim, Boram
Seo, Eun-Young
Park, Jong-Seok
Domier, Leslie L
Hammond, John
Song, Kihak
Lim, Hyoun-Sub
author_sort Choi, Go-Woon
collection PubMed
description In 2017, two new tomato mosaic virus (ToMV) isolates were collected from greenhouses in Buyeo, Chungcheongnam-do, South Korea. Full-length cDNAs of the new ToMV isolates were cloned into dual cauliflower mosaic virus 35S and T7 promoter-driven vectors, sequenced and their pathogenicities investigated. The nucleotide sequences of isolates GW1 (MH507165) and GW2 (MH507166) were 99% identical, resulting in only two amino acid differences in nonconserved region II and the helicase domain, Ile668Thr and Val834Ile. The two isolates were most closely related to a ToMV isolate from Taiwan (KJ207374). Isolate GW1 (Ile668, Val834) induced a systemic hypersensitive response in Nicotiana benthamiana compared with the isolate GW2, which a single residue substitution showed was due to Val834.
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spelling pubmed-67884072019-10-18 Full-Length Infectious Clones of Two New Isolates of Tomato Mosaic Virus Induce Distinct Symptoms Associated with Two Differential Amino Acid Residues in 128-kDa Protein Choi, Go-Woon Oh, June-Pyo Cho, In-Sook Ju, Hye-Kyoung Hu, Wen-Xing Kim, Boram Seo, Eun-Young Park, Jong-Seok Domier, Leslie L Hammond, John Song, Kihak Lim, Hyoun-Sub Plant Pathol J Note In 2017, two new tomato mosaic virus (ToMV) isolates were collected from greenhouses in Buyeo, Chungcheongnam-do, South Korea. Full-length cDNAs of the new ToMV isolates were cloned into dual cauliflower mosaic virus 35S and T7 promoter-driven vectors, sequenced and their pathogenicities investigated. The nucleotide sequences of isolates GW1 (MH507165) and GW2 (MH507166) were 99% identical, resulting in only two amino acid differences in nonconserved region II and the helicase domain, Ile668Thr and Val834Ile. The two isolates were most closely related to a ToMV isolate from Taiwan (KJ207374). Isolate GW1 (Ile668, Val834) induced a systemic hypersensitive response in Nicotiana benthamiana compared with the isolate GW2, which a single residue substitution showed was due to Val834. Korean Society of Plant Pathology 2019-10 2019-10-01 /pmc/articles/PMC6788407/ /pubmed/31632228 http://dx.doi.org/10.5423/PPJ.NT.12.2018.0286 Text en © The Korean Society of Plant Pathology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Note
Choi, Go-Woon
Oh, June-Pyo
Cho, In-Sook
Ju, Hye-Kyoung
Hu, Wen-Xing
Kim, Boram
Seo, Eun-Young
Park, Jong-Seok
Domier, Leslie L
Hammond, John
Song, Kihak
Lim, Hyoun-Sub
Full-Length Infectious Clones of Two New Isolates of Tomato Mosaic Virus Induce Distinct Symptoms Associated with Two Differential Amino Acid Residues in 128-kDa Protein
title Full-Length Infectious Clones of Two New Isolates of Tomato Mosaic Virus Induce Distinct Symptoms Associated with Two Differential Amino Acid Residues in 128-kDa Protein
title_full Full-Length Infectious Clones of Two New Isolates of Tomato Mosaic Virus Induce Distinct Symptoms Associated with Two Differential Amino Acid Residues in 128-kDa Protein
title_fullStr Full-Length Infectious Clones of Two New Isolates of Tomato Mosaic Virus Induce Distinct Symptoms Associated with Two Differential Amino Acid Residues in 128-kDa Protein
title_full_unstemmed Full-Length Infectious Clones of Two New Isolates of Tomato Mosaic Virus Induce Distinct Symptoms Associated with Two Differential Amino Acid Residues in 128-kDa Protein
title_short Full-Length Infectious Clones of Two New Isolates of Tomato Mosaic Virus Induce Distinct Symptoms Associated with Two Differential Amino Acid Residues in 128-kDa Protein
title_sort full-length infectious clones of two new isolates of tomato mosaic virus induce distinct symptoms associated with two differential amino acid residues in 128-kda protein
topic Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6788407/
https://www.ncbi.nlm.nih.gov/pubmed/31632228
http://dx.doi.org/10.5423/PPJ.NT.12.2018.0286
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