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Rice bran oil ameliorates inflammatory responses by enhancing mitochondrial respiration in murine macrophages

Previous studies have revealed the anti-inflammatory properties of rice bran oil (RBO), but the detailed mechanisms are poorly understood. Recent studies on the molecular/cellular anti-inflammatory mechanisms of dietary components have demonstrated that mitochondrial respiration plays a key role in...

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Autores principales: Lee, Sojung, Yu, Seungmin, Park, Hye Jeong, Jung, Jiyeon, Go, Gwang-woong, Kim, Wooki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6788716/
https://www.ncbi.nlm.nih.gov/pubmed/31603952
http://dx.doi.org/10.1371/journal.pone.0222857
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author Lee, Sojung
Yu, Seungmin
Park, Hye Jeong
Jung, Jiyeon
Go, Gwang-woong
Kim, Wooki
author_facet Lee, Sojung
Yu, Seungmin
Park, Hye Jeong
Jung, Jiyeon
Go, Gwang-woong
Kim, Wooki
author_sort Lee, Sojung
collection PubMed
description Previous studies have revealed the anti-inflammatory properties of rice bran oil (RBO), but the detailed mechanisms are poorly understood. Recent studies on the molecular/cellular anti-inflammatory mechanisms of dietary components have demonstrated that mitochondrial respiration plays a key role in macrophage functioning. Since dietary lipids are major substrates for mitochondrial respiration through β-oxidation, the current study examined whether RBO regulates inflammatory responses by modulating mitochondrial energy metabolism. Palm oil (PO), enriched with palmitic acid which are known to be effectively taken up by cells and used for oxidative phosphorylation, served as a positive control. In the in vitro model of LPS-stimulated RAW 264.7 murine cells, the levels of pro-inflammatory cytokines (IL-6 and TNF-α) in the culture supernatant were significantly reduced by RBO treatment. In contrast, secretion of the anti-inflammatory cytokine IL-10 was upregulated by RBO. Transcription of genes encoding inflammatory mediator molecules (COX-2 and iNOS) and expression of activation markers (CD80, CD86, and MHC-II) in LPS-stimulated RAW 264.7 cells were suppressed by RBO. Mitochondrial respiration (as assessed by an extracellular flux analyzer) increased upon RBO treatment, as the basal respiration, maximal respiration, ATP production, and spare respiratory capacity were upregulated. In an in vivo study, C57BL/6 mice were fed a negative control diet containing corn oil (CO), PO, or RBO for 4 weeks, and bone marrow-derived macrophages (BMDM) were isolated from their tibias and femurs. In pro-inflammatory M1-polarized BMDM (M1-BMDM), the RBO-induced suppression of IL-6 and TNF-α was recapitulated in vivo. Mitochondrial respiration in M1-BMDM also increased following the RBO intervention and the PO control treatment as compared to CO fed negative control. Overall, the current study for the first time demonstrates that RBO regulates inflammatory responses in murine macrophages by upregulating mitochondrial respiration. Further clinical studies are required to validate the animal study.
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spelling pubmed-67887162019-10-25 Rice bran oil ameliorates inflammatory responses by enhancing mitochondrial respiration in murine macrophages Lee, Sojung Yu, Seungmin Park, Hye Jeong Jung, Jiyeon Go, Gwang-woong Kim, Wooki PLoS One Research Article Previous studies have revealed the anti-inflammatory properties of rice bran oil (RBO), but the detailed mechanisms are poorly understood. Recent studies on the molecular/cellular anti-inflammatory mechanisms of dietary components have demonstrated that mitochondrial respiration plays a key role in macrophage functioning. Since dietary lipids are major substrates for mitochondrial respiration through β-oxidation, the current study examined whether RBO regulates inflammatory responses by modulating mitochondrial energy metabolism. Palm oil (PO), enriched with palmitic acid which are known to be effectively taken up by cells and used for oxidative phosphorylation, served as a positive control. In the in vitro model of LPS-stimulated RAW 264.7 murine cells, the levels of pro-inflammatory cytokines (IL-6 and TNF-α) in the culture supernatant were significantly reduced by RBO treatment. In contrast, secretion of the anti-inflammatory cytokine IL-10 was upregulated by RBO. Transcription of genes encoding inflammatory mediator molecules (COX-2 and iNOS) and expression of activation markers (CD80, CD86, and MHC-II) in LPS-stimulated RAW 264.7 cells were suppressed by RBO. Mitochondrial respiration (as assessed by an extracellular flux analyzer) increased upon RBO treatment, as the basal respiration, maximal respiration, ATP production, and spare respiratory capacity were upregulated. In an in vivo study, C57BL/6 mice were fed a negative control diet containing corn oil (CO), PO, or RBO for 4 weeks, and bone marrow-derived macrophages (BMDM) were isolated from their tibias and femurs. In pro-inflammatory M1-polarized BMDM (M1-BMDM), the RBO-induced suppression of IL-6 and TNF-α was recapitulated in vivo. Mitochondrial respiration in M1-BMDM also increased following the RBO intervention and the PO control treatment as compared to CO fed negative control. Overall, the current study for the first time demonstrates that RBO regulates inflammatory responses in murine macrophages by upregulating mitochondrial respiration. Further clinical studies are required to validate the animal study. Public Library of Science 2019-10-11 /pmc/articles/PMC6788716/ /pubmed/31603952 http://dx.doi.org/10.1371/journal.pone.0222857 Text en © 2019 Lee et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Lee, Sojung
Yu, Seungmin
Park, Hye Jeong
Jung, Jiyeon
Go, Gwang-woong
Kim, Wooki
Rice bran oil ameliorates inflammatory responses by enhancing mitochondrial respiration in murine macrophages
title Rice bran oil ameliorates inflammatory responses by enhancing mitochondrial respiration in murine macrophages
title_full Rice bran oil ameliorates inflammatory responses by enhancing mitochondrial respiration in murine macrophages
title_fullStr Rice bran oil ameliorates inflammatory responses by enhancing mitochondrial respiration in murine macrophages
title_full_unstemmed Rice bran oil ameliorates inflammatory responses by enhancing mitochondrial respiration in murine macrophages
title_short Rice bran oil ameliorates inflammatory responses by enhancing mitochondrial respiration in murine macrophages
title_sort rice bran oil ameliorates inflammatory responses by enhancing mitochondrial respiration in murine macrophages
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6788716/
https://www.ncbi.nlm.nih.gov/pubmed/31603952
http://dx.doi.org/10.1371/journal.pone.0222857
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