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Impelling force and current challenges by chemicals in somatic cell reprogramming and expansion beyond hepatocytes
In the field of regenerative medicine, generating numerous transplantable functional cells in the laboratory setting on a large scale is a major challenge. However, the in vitro maintenance and expansion of terminally differentiated cells are challenging because of the lack of specific environmental...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Baishideng Publishing Group Inc
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6789182/ https://www.ncbi.nlm.nih.gov/pubmed/31616541 http://dx.doi.org/10.4252/wjsc.v11.i9.650 |
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author | Ge, Jian-Yun Zheng, Yun-Wen Liu, Li-Ping Isoda, Hiroko Oda, Tatsuya |
author_facet | Ge, Jian-Yun Zheng, Yun-Wen Liu, Li-Ping Isoda, Hiroko Oda, Tatsuya |
author_sort | Ge, Jian-Yun |
collection | PubMed |
description | In the field of regenerative medicine, generating numerous transplantable functional cells in the laboratory setting on a large scale is a major challenge. However, the in vitro maintenance and expansion of terminally differentiated cells are challenging because of the lack of specific environmental and intercellular signal stimulations, markedly hindering their therapeutic application. Remarkably, the generation of stem/progenitor cells or functional cells with effective proliferative potential is markedly in demand for disease modeling, cell-based transplantation, and drug discovery. Despite the potent genetic manipulation of transcription factors, integration-free chemically defined approaches for the conversion of somatic cell fate have garnered considerable attention in recent years. This review aims to summarize the progress thus far and discuss the advantages, limitations, and challenges of the impact of full chemicals on the stepwise reprogramming of pluripotency, direct lineage conversion, and direct lineage expansion on somatic cells. Owing to the current chemical-mediated induction, reprogrammed pluripotent stem cells with reproducibility difficulties, and direct lineage converted cells with marked functional deficiency, it is imperative to generate the desired cell types directly by chemically inducing their potent proliferation ability through a lineage-committed progenitor state, while upholding the maturation and engraftment capacity posttransplantation in vivo. Together with the comprehensive understanding of the mechanism of chemical drives, as well as the elucidation of specificity and commonalities, the precise manipulation of the expansion for diverse functional cell types could broaden the available cell sources and enhance the cellular function for clinical application in future. |
format | Online Article Text |
id | pubmed-6789182 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Baishideng Publishing Group Inc |
record_format | MEDLINE/PubMed |
spelling | pubmed-67891822019-10-15 Impelling force and current challenges by chemicals in somatic cell reprogramming and expansion beyond hepatocytes Ge, Jian-Yun Zheng, Yun-Wen Liu, Li-Ping Isoda, Hiroko Oda, Tatsuya World J Stem Cells Review In the field of regenerative medicine, generating numerous transplantable functional cells in the laboratory setting on a large scale is a major challenge. However, the in vitro maintenance and expansion of terminally differentiated cells are challenging because of the lack of specific environmental and intercellular signal stimulations, markedly hindering their therapeutic application. Remarkably, the generation of stem/progenitor cells or functional cells with effective proliferative potential is markedly in demand for disease modeling, cell-based transplantation, and drug discovery. Despite the potent genetic manipulation of transcription factors, integration-free chemically defined approaches for the conversion of somatic cell fate have garnered considerable attention in recent years. This review aims to summarize the progress thus far and discuss the advantages, limitations, and challenges of the impact of full chemicals on the stepwise reprogramming of pluripotency, direct lineage conversion, and direct lineage expansion on somatic cells. Owing to the current chemical-mediated induction, reprogrammed pluripotent stem cells with reproducibility difficulties, and direct lineage converted cells with marked functional deficiency, it is imperative to generate the desired cell types directly by chemically inducing their potent proliferation ability through a lineage-committed progenitor state, while upholding the maturation and engraftment capacity posttransplantation in vivo. Together with the comprehensive understanding of the mechanism of chemical drives, as well as the elucidation of specificity and commonalities, the precise manipulation of the expansion for diverse functional cell types could broaden the available cell sources and enhance the cellular function for clinical application in future. Baishideng Publishing Group Inc 2019-09-26 2019-09-26 /pmc/articles/PMC6789182/ /pubmed/31616541 http://dx.doi.org/10.4252/wjsc.v11.i9.650 Text en ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. |
spellingShingle | Review Ge, Jian-Yun Zheng, Yun-Wen Liu, Li-Ping Isoda, Hiroko Oda, Tatsuya Impelling force and current challenges by chemicals in somatic cell reprogramming and expansion beyond hepatocytes |
title | Impelling force and current challenges by chemicals in somatic cell reprogramming and expansion beyond hepatocytes |
title_full | Impelling force and current challenges by chemicals in somatic cell reprogramming and expansion beyond hepatocytes |
title_fullStr | Impelling force and current challenges by chemicals in somatic cell reprogramming and expansion beyond hepatocytes |
title_full_unstemmed | Impelling force and current challenges by chemicals in somatic cell reprogramming and expansion beyond hepatocytes |
title_short | Impelling force and current challenges by chemicals in somatic cell reprogramming and expansion beyond hepatocytes |
title_sort | impelling force and current challenges by chemicals in somatic cell reprogramming and expansion beyond hepatocytes |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6789182/ https://www.ncbi.nlm.nih.gov/pubmed/31616541 http://dx.doi.org/10.4252/wjsc.v11.i9.650 |
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