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Geographical Distribution of β-Lactam Resistance among Klebsiella spp. from Selected Health Facilities in Ghana

β-Lactam-resistant Klebsiella isolates continue to cause multidrug resistance infections worldwide. This study aimed to describe the geographical distribution of extended spectrum β-lactamase (ESBL), AmpC β-lactamase (AmpC), and carbapenemase production among 139 Klebsiella isolates recovered from p...

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Autores principales: Quansah, Elvis, Amoah Barnie, Prince, Omane Acheampong, Desmond, Obiri-Yeboah, Dorcas, Odarkor Mills, Richael, Asmah, Ebenezer, Cudjoe, Obed, Dadzie, Isaac
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6789473/
https://www.ncbi.nlm.nih.gov/pubmed/31484298
http://dx.doi.org/10.3390/tropicalmed4030117
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author Quansah, Elvis
Amoah Barnie, Prince
Omane Acheampong, Desmond
Obiri-Yeboah, Dorcas
Odarkor Mills, Richael
Asmah, Ebenezer
Cudjoe, Obed
Dadzie, Isaac
author_facet Quansah, Elvis
Amoah Barnie, Prince
Omane Acheampong, Desmond
Obiri-Yeboah, Dorcas
Odarkor Mills, Richael
Asmah, Ebenezer
Cudjoe, Obed
Dadzie, Isaac
author_sort Quansah, Elvis
collection PubMed
description β-Lactam-resistant Klebsiella isolates continue to cause multidrug resistance infections worldwide. This study aimed to describe the geographical distribution of extended spectrum β-lactamase (ESBL), AmpC β-lactamase (AmpC), and carbapenemase production among 139 Klebsiella isolates recovered from patients at major referral health facilities in Ghana. The phenotypic methods of combined disc diffusion test, modified three-dimensional test, modified Hodge test (MHT), and combined disc test were performed for each isolate to detect ESBL, AmpC, carbapenemase, and metallo-β-lactamase (MBL) producers, respectively. Except for MBL, all other β-lactam resistance mechanisms were highest in the healthcare facilities situated in the northern belt of Ghana. Significant regional difference of ESBL producers was observed between the northern and middle belts as well as the northern and southern belts. Genotypic detection with polymerase chain reaction (PCR) revealed the presence of bla TEM 36/139 (25.9%), bla SHV 40/139 (28.8%), bla CTX-M 37/139 (26.6%), bla OXA-48 3/139 (2.16%), and bla NDM 1/139 (0.72%) genotypes. In conclusion, there were variations in β-lactam resistance among Klebsiella spp. from health facilities situated in the northern, middle, and southern belts of Ghana. The study provides preliminary evidence that emphasizes the need to direct more attention to antimicrobial resistance control, especially in the northern belt of Ghana. Findings from this study may be critical for creating and fine-tuning effective antimicrobial resistance control strategies and for informing accurate antibiotic prescription by practitioners.
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spelling pubmed-67894732019-10-16 Geographical Distribution of β-Lactam Resistance among Klebsiella spp. from Selected Health Facilities in Ghana Quansah, Elvis Amoah Barnie, Prince Omane Acheampong, Desmond Obiri-Yeboah, Dorcas Odarkor Mills, Richael Asmah, Ebenezer Cudjoe, Obed Dadzie, Isaac Trop Med Infect Dis Article β-Lactam-resistant Klebsiella isolates continue to cause multidrug resistance infections worldwide. This study aimed to describe the geographical distribution of extended spectrum β-lactamase (ESBL), AmpC β-lactamase (AmpC), and carbapenemase production among 139 Klebsiella isolates recovered from patients at major referral health facilities in Ghana. The phenotypic methods of combined disc diffusion test, modified three-dimensional test, modified Hodge test (MHT), and combined disc test were performed for each isolate to detect ESBL, AmpC, carbapenemase, and metallo-β-lactamase (MBL) producers, respectively. Except for MBL, all other β-lactam resistance mechanisms were highest in the healthcare facilities situated in the northern belt of Ghana. Significant regional difference of ESBL producers was observed between the northern and middle belts as well as the northern and southern belts. Genotypic detection with polymerase chain reaction (PCR) revealed the presence of bla TEM 36/139 (25.9%), bla SHV 40/139 (28.8%), bla CTX-M 37/139 (26.6%), bla OXA-48 3/139 (2.16%), and bla NDM 1/139 (0.72%) genotypes. In conclusion, there were variations in β-lactam resistance among Klebsiella spp. from health facilities situated in the northern, middle, and southern belts of Ghana. The study provides preliminary evidence that emphasizes the need to direct more attention to antimicrobial resistance control, especially in the northern belt of Ghana. Findings from this study may be critical for creating and fine-tuning effective antimicrobial resistance control strategies and for informing accurate antibiotic prescription by practitioners. MDPI 2019-09-03 /pmc/articles/PMC6789473/ /pubmed/31484298 http://dx.doi.org/10.3390/tropicalmed4030117 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Quansah, Elvis
Amoah Barnie, Prince
Omane Acheampong, Desmond
Obiri-Yeboah, Dorcas
Odarkor Mills, Richael
Asmah, Ebenezer
Cudjoe, Obed
Dadzie, Isaac
Geographical Distribution of β-Lactam Resistance among Klebsiella spp. from Selected Health Facilities in Ghana
title Geographical Distribution of β-Lactam Resistance among Klebsiella spp. from Selected Health Facilities in Ghana
title_full Geographical Distribution of β-Lactam Resistance among Klebsiella spp. from Selected Health Facilities in Ghana
title_fullStr Geographical Distribution of β-Lactam Resistance among Klebsiella spp. from Selected Health Facilities in Ghana
title_full_unstemmed Geographical Distribution of β-Lactam Resistance among Klebsiella spp. from Selected Health Facilities in Ghana
title_short Geographical Distribution of β-Lactam Resistance among Klebsiella spp. from Selected Health Facilities in Ghana
title_sort geographical distribution of β-lactam resistance among klebsiella spp. from selected health facilities in ghana
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6789473/
https://www.ncbi.nlm.nih.gov/pubmed/31484298
http://dx.doi.org/10.3390/tropicalmed4030117
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