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Large Field Alpha Irradiation Setup for Radiobiological Experiments
The use of alpha particles irradiation in clinical practice has gained interest in the past years, for example with the advance of radionuclide therapy. The lack of affordable and easily accessible irradiation systems to study the cell biological impact of alpha particles hampers broad investigation...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6789741/ https://www.ncbi.nlm.nih.gov/pubmed/31466405 http://dx.doi.org/10.3390/mps2030075 |
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author | Roobol, Stefan J. Kouwenberg, Jasper J.M. Denkova, Antonia G. Kanaar, Roland Essers, Jeroen |
author_facet | Roobol, Stefan J. Kouwenberg, Jasper J.M. Denkova, Antonia G. Kanaar, Roland Essers, Jeroen |
author_sort | Roobol, Stefan J. |
collection | PubMed |
description | The use of alpha particles irradiation in clinical practice has gained interest in the past years, for example with the advance of radionuclide therapy. The lack of affordable and easily accessible irradiation systems to study the cell biological impact of alpha particles hampers broad investigation. Here we present a novel alpha particle irradiation set-up for uniform irradiation of cell cultures. By combining a small alpha emitting source and a computer-directed movement stage, we established a new alpha particle irradiation method allowing more advanced biological assays, including large-field local alpha particle irradiation and cell survival assays. In addition, this protocol uses cell culture on glass cover-slips which allows more advanced microscopy, such as super-resolution imaging, for in-depth analysis of the DNA damage caused by alpha particles. This novel irradiation set-up provides the possibility to perform reproducible, uniform and directed alpha particle irradiation to investigate the impact of alpha radiation on the cellular level. |
format | Online Article Text |
id | pubmed-6789741 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67897412019-10-16 Large Field Alpha Irradiation Setup for Radiobiological Experiments Roobol, Stefan J. Kouwenberg, Jasper J.M. Denkova, Antonia G. Kanaar, Roland Essers, Jeroen Methods Protoc Protocol The use of alpha particles irradiation in clinical practice has gained interest in the past years, for example with the advance of radionuclide therapy. The lack of affordable and easily accessible irradiation systems to study the cell biological impact of alpha particles hampers broad investigation. Here we present a novel alpha particle irradiation set-up for uniform irradiation of cell cultures. By combining a small alpha emitting source and a computer-directed movement stage, we established a new alpha particle irradiation method allowing more advanced biological assays, including large-field local alpha particle irradiation and cell survival assays. In addition, this protocol uses cell culture on glass cover-slips which allows more advanced microscopy, such as super-resolution imaging, for in-depth analysis of the DNA damage caused by alpha particles. This novel irradiation set-up provides the possibility to perform reproducible, uniform and directed alpha particle irradiation to investigate the impact of alpha radiation on the cellular level. MDPI 2019-08-28 /pmc/articles/PMC6789741/ /pubmed/31466405 http://dx.doi.org/10.3390/mps2030075 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Protocol Roobol, Stefan J. Kouwenberg, Jasper J.M. Denkova, Antonia G. Kanaar, Roland Essers, Jeroen Large Field Alpha Irradiation Setup for Radiobiological Experiments |
title | Large Field Alpha Irradiation Setup for Radiobiological Experiments |
title_full | Large Field Alpha Irradiation Setup for Radiobiological Experiments |
title_fullStr | Large Field Alpha Irradiation Setup for Radiobiological Experiments |
title_full_unstemmed | Large Field Alpha Irradiation Setup for Radiobiological Experiments |
title_short | Large Field Alpha Irradiation Setup for Radiobiological Experiments |
title_sort | large field alpha irradiation setup for radiobiological experiments |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6789741/ https://www.ncbi.nlm.nih.gov/pubmed/31466405 http://dx.doi.org/10.3390/mps2030075 |
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