A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals

Mammals can play a role as an intermediate host in the emergence of mammalian-adapted reassortants or mutants of avian influenza A viruses, with pandemic potential. Therefore, detecting viral infection in animals followed by assessment of the hemagglutinin (HA) subtype of the agent is an indispensab...

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Detalles Bibliográficos
Autores principales: Okumura, Miki, Takenaka-Uema, Akiko, Murakami, Shin, Horimoto, Taisuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6789898/
https://www.ncbi.nlm.nih.gov/pubmed/31336638
http://dx.doi.org/10.3390/vetsci6030064
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author Okumura, Miki
Takenaka-Uema, Akiko
Murakami, Shin
Horimoto, Taisuke
author_facet Okumura, Miki
Takenaka-Uema, Akiko
Murakami, Shin
Horimoto, Taisuke
author_sort Okumura, Miki
collection PubMed
description Mammals can play a role as an intermediate host in the emergence of mammalian-adapted reassortants or mutants of avian influenza A viruses, with pandemic potential. Therefore, detecting viral infection in animals followed by assessment of the hemagglutinin (HA) subtype of the agent is an indispensable process for risk assessment in pandemic preparedness. In this study, we tested the potential of an enzyme-linked immunosorbent assay as a rapid diagnosis method, using a panel of HA subtype antigens. By analyzing reference immune sera, we found that this novel assay could detect HA subtype-specific antibodies without considerable inter-subtypic cross-reactivities, contributing to diagnosis of influenza virus infection.
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spelling pubmed-67898982019-10-16 A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals Okumura, Miki Takenaka-Uema, Akiko Murakami, Shin Horimoto, Taisuke Vet Sci Communication Mammals can play a role as an intermediate host in the emergence of mammalian-adapted reassortants or mutants of avian influenza A viruses, with pandemic potential. Therefore, detecting viral infection in animals followed by assessment of the hemagglutinin (HA) subtype of the agent is an indispensable process for risk assessment in pandemic preparedness. In this study, we tested the potential of an enzyme-linked immunosorbent assay as a rapid diagnosis method, using a panel of HA subtype antigens. By analyzing reference immune sera, we found that this novel assay could detect HA subtype-specific antibodies without considerable inter-subtypic cross-reactivities, contributing to diagnosis of influenza virus infection. MDPI 2019-07-22 /pmc/articles/PMC6789898/ /pubmed/31336638 http://dx.doi.org/10.3390/vetsci6030064 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Okumura, Miki
Takenaka-Uema, Akiko
Murakami, Shin
Horimoto, Taisuke
A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals
title A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals
title_full A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals
title_fullStr A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals
title_full_unstemmed A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals
title_short A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals
title_sort multi-hemagglutinin-based enzyme-linked immunosorbent assay to serologically detect influenza a virus infection in animals
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6789898/
https://www.ncbi.nlm.nih.gov/pubmed/31336638
http://dx.doi.org/10.3390/vetsci6030064
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