Cargando…
Highly efficient transgene‐free targeted mutagenesis and single‐stranded oligodeoxynucleotide‐mediated precise knock‐in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2019
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6790356/ https://www.ncbi.nlm.nih.gov/pubmed/31131534 http://dx.doi.org/10.1111/pbi.13174 |
| _version_ | 1783458782182899712 |
|---|---|
| author | Nomura, Toshihisa Inoue, Komaki Uehara‐Yamaguchi, Yukiko Yamada, Koji Iwata, Osamu Suzuki, Kengo Mochida, Keiichi |
| author_facet | Nomura, Toshihisa Inoue, Komaki Uehara‐Yamaguchi, Yukiko Yamada, Koji Iwata, Osamu Suzuki, Kengo Mochida, Keiichi |
| author_sort | Nomura, Toshihisa |
| collection | PubMed |
| description | |
| format | Online Article Text |
| id | pubmed-6790356 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2019 |
| publisher | John Wiley and Sons Inc. |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-67903562019-10-18 Highly efficient transgene‐free targeted mutagenesis and single‐stranded oligodeoxynucleotide‐mediated precise knock‐in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins Nomura, Toshihisa Inoue, Komaki Uehara‐Yamaguchi, Yukiko Yamada, Koji Iwata, Osamu Suzuki, Kengo Mochida, Keiichi Plant Biotechnol J Editorials John Wiley and Sons Inc. 2019-06-11 2019-11 /pmc/articles/PMC6790356/ /pubmed/31131534 http://dx.doi.org/10.1111/pbi.13174 Text en © 2019 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Editorials Nomura, Toshihisa Inoue, Komaki Uehara‐Yamaguchi, Yukiko Yamada, Koji Iwata, Osamu Suzuki, Kengo Mochida, Keiichi Highly efficient transgene‐free targeted mutagenesis and single‐stranded oligodeoxynucleotide‐mediated precise knock‐in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins |
| title | Highly efficient transgene‐free targeted mutagenesis and single‐stranded oligodeoxynucleotide‐mediated precise knock‐in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins |
| title_full | Highly efficient transgene‐free targeted mutagenesis and single‐stranded oligodeoxynucleotide‐mediated precise knock‐in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins |
| title_fullStr | Highly efficient transgene‐free targeted mutagenesis and single‐stranded oligodeoxynucleotide‐mediated precise knock‐in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins |
| title_full_unstemmed | Highly efficient transgene‐free targeted mutagenesis and single‐stranded oligodeoxynucleotide‐mediated precise knock‐in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins |
| title_short | Highly efficient transgene‐free targeted mutagenesis and single‐stranded oligodeoxynucleotide‐mediated precise knock‐in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins |
| title_sort | highly efficient transgene‐free targeted mutagenesis and single‐stranded oligodeoxynucleotide‐mediated precise knock‐in in the industrial microalga euglena gracilis using cas9 ribonucleoproteins |
| topic | Editorials |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6790356/ https://www.ncbi.nlm.nih.gov/pubmed/31131534 http://dx.doi.org/10.1111/pbi.13174 |
| work_keys_str_mv | AT nomuratoshihisa highlyefficienttransgenefreetargetedmutagenesisandsinglestrandedoligodeoxynucleotidemediatedpreciseknockinintheindustrialmicroalgaeuglenagracilisusingcas9ribonucleoproteins AT inouekomaki highlyefficienttransgenefreetargetedmutagenesisandsinglestrandedoligodeoxynucleotidemediatedpreciseknockinintheindustrialmicroalgaeuglenagracilisusingcas9ribonucleoproteins AT ueharayamaguchiyukiko highlyefficienttransgenefreetargetedmutagenesisandsinglestrandedoligodeoxynucleotidemediatedpreciseknockinintheindustrialmicroalgaeuglenagracilisusingcas9ribonucleoproteins AT yamadakoji highlyefficienttransgenefreetargetedmutagenesisandsinglestrandedoligodeoxynucleotidemediatedpreciseknockinintheindustrialmicroalgaeuglenagracilisusingcas9ribonucleoproteins AT iwataosamu highlyefficienttransgenefreetargetedmutagenesisandsinglestrandedoligodeoxynucleotidemediatedpreciseknockinintheindustrialmicroalgaeuglenagracilisusingcas9ribonucleoproteins AT suzukikengo highlyefficienttransgenefreetargetedmutagenesisandsinglestrandedoligodeoxynucleotidemediatedpreciseknockinintheindustrialmicroalgaeuglenagracilisusingcas9ribonucleoproteins AT mochidakeiichi highlyefficienttransgenefreetargetedmutagenesisandsinglestrandedoligodeoxynucleotidemediatedpreciseknockinintheindustrialmicroalgaeuglenagracilisusingcas9ribonucleoproteins |