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INPP4B As A Prognostic And Diagnostic Marker Regulates Cell Growth Of Pancreatic Cancer Via Activating AKT

BACKGROUND: Inositol polyphosphate 4-phosphatase type II (INPP4B), a member of the PI3K/Akt signaling pathway, plays a vital role in the initiation and progression of cancers. However, its biological role in pancreatic cancer remains largely undiscovered. Our study aimed to investigate the effects o...

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Autores principales: Zhai, Shuyu, Liu, Yuanbin, Lu, Xiongxiong, Qian, Hao, Tang, Xiaomei, Cheng, Xi, Wang, Yue, Shi, Yusheng, Deng, Xiaxing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6790406/
https://www.ncbi.nlm.nih.gov/pubmed/31632078
http://dx.doi.org/10.2147/OTT.S223221
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author Zhai, Shuyu
Liu, Yuanbin
Lu, Xiongxiong
Qian, Hao
Tang, Xiaomei
Cheng, Xi
Wang, Yue
Shi, Yusheng
Deng, Xiaxing
author_facet Zhai, Shuyu
Liu, Yuanbin
Lu, Xiongxiong
Qian, Hao
Tang, Xiaomei
Cheng, Xi
Wang, Yue
Shi, Yusheng
Deng, Xiaxing
author_sort Zhai, Shuyu
collection PubMed
description BACKGROUND: Inositol polyphosphate 4-phosphatase type II (INPP4B), a member of the PI3K/Akt signaling pathway, plays a vital role in the initiation and progression of cancers. However, its biological role in pancreatic cancer remains largely undiscovered. Our study aimed to investigate the effects of INPP4B on proliferation in pancreatic cancer and its clinical relevance. MATERIALS AND METHODS: INPP4B expression data were obtained from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Clinicopathological and survival data were retrieved from the TCGA database. CCK8 and colony formation assays were performed to measure the proliferative capacity of pancreatic cancer. Tumor xenograft models were established to measure cancer proliferative abilities in vivo. RESULTS: INPP4B was upregulated in pancreatic cancer tissue compared with normal tissue. INPP4B knockdown inhibited cell proliferation and promoted apoptosis in pancreatic cancer in vitro and in vivo. INPP4B knockdown also reduced AKT phosphorylation. Moreover, INPP4B was associated with poor overall and disease-free survival, with Cox regression analysis showing that INPP4B could serve as an independent prognostic marker. ROC curve analysis showed that INPP4B possessed moderate diagnostic value. CONCLUSION: Collectively, INPP4B is an oncogenic gene in pancreatic cancer and could serve as a potential diagnostic marker and an independent prognostic marker, suggesting that it could be a novel therapeutic target for pancreatic cancer.
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spelling pubmed-67904062019-10-18 INPP4B As A Prognostic And Diagnostic Marker Regulates Cell Growth Of Pancreatic Cancer Via Activating AKT Zhai, Shuyu Liu, Yuanbin Lu, Xiongxiong Qian, Hao Tang, Xiaomei Cheng, Xi Wang, Yue Shi, Yusheng Deng, Xiaxing Onco Targets Ther Original Research BACKGROUND: Inositol polyphosphate 4-phosphatase type II (INPP4B), a member of the PI3K/Akt signaling pathway, plays a vital role in the initiation and progression of cancers. However, its biological role in pancreatic cancer remains largely undiscovered. Our study aimed to investigate the effects of INPP4B on proliferation in pancreatic cancer and its clinical relevance. MATERIALS AND METHODS: INPP4B expression data were obtained from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Clinicopathological and survival data were retrieved from the TCGA database. CCK8 and colony formation assays were performed to measure the proliferative capacity of pancreatic cancer. Tumor xenograft models were established to measure cancer proliferative abilities in vivo. RESULTS: INPP4B was upregulated in pancreatic cancer tissue compared with normal tissue. INPP4B knockdown inhibited cell proliferation and promoted apoptosis in pancreatic cancer in vitro and in vivo. INPP4B knockdown also reduced AKT phosphorylation. Moreover, INPP4B was associated with poor overall and disease-free survival, with Cox regression analysis showing that INPP4B could serve as an independent prognostic marker. ROC curve analysis showed that INPP4B possessed moderate diagnostic value. CONCLUSION: Collectively, INPP4B is an oncogenic gene in pancreatic cancer and could serve as a potential diagnostic marker and an independent prognostic marker, suggesting that it could be a novel therapeutic target for pancreatic cancer. Dove 2019-10-09 /pmc/articles/PMC6790406/ /pubmed/31632078 http://dx.doi.org/10.2147/OTT.S223221 Text en © 2019 Zhai et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Zhai, Shuyu
Liu, Yuanbin
Lu, Xiongxiong
Qian, Hao
Tang, Xiaomei
Cheng, Xi
Wang, Yue
Shi, Yusheng
Deng, Xiaxing
INPP4B As A Prognostic And Diagnostic Marker Regulates Cell Growth Of Pancreatic Cancer Via Activating AKT
title INPP4B As A Prognostic And Diagnostic Marker Regulates Cell Growth Of Pancreatic Cancer Via Activating AKT
title_full INPP4B As A Prognostic And Diagnostic Marker Regulates Cell Growth Of Pancreatic Cancer Via Activating AKT
title_fullStr INPP4B As A Prognostic And Diagnostic Marker Regulates Cell Growth Of Pancreatic Cancer Via Activating AKT
title_full_unstemmed INPP4B As A Prognostic And Diagnostic Marker Regulates Cell Growth Of Pancreatic Cancer Via Activating AKT
title_short INPP4B As A Prognostic And Diagnostic Marker Regulates Cell Growth Of Pancreatic Cancer Via Activating AKT
title_sort inpp4b as a prognostic and diagnostic marker regulates cell growth of pancreatic cancer via activating akt
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6790406/
https://www.ncbi.nlm.nih.gov/pubmed/31632078
http://dx.doi.org/10.2147/OTT.S223221
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