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Characterization of biological variation of peripheral blood immune cytome in an Indian cohort

Immune parameters show characteristic normal baseline levels and variance in the population. We characterised the degree of inter-individual and within-individual variation over one-year time period in 33 immune cell subsets by flow cytometry in peripheral blood mononuclear cells from 43 healthy you...

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Autores principales: Kanodia, Parna, Kaur, Gurvinder, Coshic, Poonam, Chatterjee, Kabita, Neeman, Teresa, George, Anna, Rath, Satyajit, Bal, Vineeta, Prabhu, Savit B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6791881/
https://www.ncbi.nlm.nih.gov/pubmed/31611580
http://dx.doi.org/10.1038/s41598-019-51294-7
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author Kanodia, Parna
Kaur, Gurvinder
Coshic, Poonam
Chatterjee, Kabita
Neeman, Teresa
George, Anna
Rath, Satyajit
Bal, Vineeta
Prabhu, Savit B.
author_facet Kanodia, Parna
Kaur, Gurvinder
Coshic, Poonam
Chatterjee, Kabita
Neeman, Teresa
George, Anna
Rath, Satyajit
Bal, Vineeta
Prabhu, Savit B.
author_sort Kanodia, Parna
collection PubMed
description Immune parameters show characteristic normal baseline levels and variance in the population. We characterised the degree of inter-individual and within-individual variation over one-year time period in 33 immune cell subsets by flow cytometry in peripheral blood mononuclear cells from 43 healthy young adult volunteers. Our analysis revealed that immune subsets that showed low variability between individuals also showed low short-term fluctuations within-individuals, as well as concordance in siblings. However, when baseline levels and degree of fluctuation were considered together, individuals failed to cluster into discreet groups. Together, the data reveal complex inter-relationships between immune subsets in individuals, and provide insights into the observed heterogeneity between individuals and between multiple immune subsets.
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spelling pubmed-67918812019-10-21 Characterization of biological variation of peripheral blood immune cytome in an Indian cohort Kanodia, Parna Kaur, Gurvinder Coshic, Poonam Chatterjee, Kabita Neeman, Teresa George, Anna Rath, Satyajit Bal, Vineeta Prabhu, Savit B. Sci Rep Article Immune parameters show characteristic normal baseline levels and variance in the population. We characterised the degree of inter-individual and within-individual variation over one-year time period in 33 immune cell subsets by flow cytometry in peripheral blood mononuclear cells from 43 healthy young adult volunteers. Our analysis revealed that immune subsets that showed low variability between individuals also showed low short-term fluctuations within-individuals, as well as concordance in siblings. However, when baseline levels and degree of fluctuation were considered together, individuals failed to cluster into discreet groups. Together, the data reveal complex inter-relationships between immune subsets in individuals, and provide insights into the observed heterogeneity between individuals and between multiple immune subsets. Nature Publishing Group UK 2019-10-14 /pmc/articles/PMC6791881/ /pubmed/31611580 http://dx.doi.org/10.1038/s41598-019-51294-7 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kanodia, Parna
Kaur, Gurvinder
Coshic, Poonam
Chatterjee, Kabita
Neeman, Teresa
George, Anna
Rath, Satyajit
Bal, Vineeta
Prabhu, Savit B.
Characterization of biological variation of peripheral blood immune cytome in an Indian cohort
title Characterization of biological variation of peripheral blood immune cytome in an Indian cohort
title_full Characterization of biological variation of peripheral blood immune cytome in an Indian cohort
title_fullStr Characterization of biological variation of peripheral blood immune cytome in an Indian cohort
title_full_unstemmed Characterization of biological variation of peripheral blood immune cytome in an Indian cohort
title_short Characterization of biological variation of peripheral blood immune cytome in an Indian cohort
title_sort characterization of biological variation of peripheral blood immune cytome in an indian cohort
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6791881/
https://www.ncbi.nlm.nih.gov/pubmed/31611580
http://dx.doi.org/10.1038/s41598-019-51294-7
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