Rational development of transformation in Clostridium thermocellum ATCC 27405 via complete methylome analysis and evasion of native restriction–modification systems

A major barrier to both metabolic engineering and fundamental biological studies is the lack of genetic tools in most microorganisms. One example is Clostridium thermocellum ATCC 27405(T), where genetic tools are not available to help validate decades of hypotheses. A significant barrier to DNA tran...

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Detalles Bibliográficos
Autores principales: Riley, Lauren A., Ji, Lexiang, Schmitz, Robert J., Westpheling, Janet, Guss, Adam M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2019
Materias:
Genetics and Molecular Biology of Industrial Organisms - Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6791906/
https://www.ncbi.nlm.nih.gov/pubmed/31342224
http://dx.doi.org/10.1007/s10295-019-02218-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6791906/
https://www.ncbi.nlm.nih.gov/pubmed/31342224
http://dx.doi.org/10.1007/s10295-019-02218-x

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