Cargando…

Regulation of Hormone-Related Genes in Ericerus pela (Hemiptera: Coccidae) for Dimorphic Metamorphosis

Insect hormones regulate metamorphosis including that leading to sexual dimorphism. Using RNA-Seq, we discovered that the second-instar male larva (SM) of the white wax insect, Ericerus pela, have 5,968 and 8,620 differentially expressed transcripts compared with the second-instar female larva (SF)...

Descripción completa

Detalles Bibliográficos
Autores principales: Pengfei, Liu, Weiwei, Wang, Xiaofei, Ling, Qin, Lu, Jinwen, Zhang, Rui, He, Hang, Chen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6792091/
https://www.ncbi.nlm.nih.gov/pubmed/31612946
http://dx.doi.org/10.1093/jisesa/iez092
_version_ 1783459080802664448
author Pengfei, Liu
Weiwei, Wang
Xiaofei, Ling
Qin, Lu
Jinwen, Zhang
Rui, He
Hang, Chen
author_facet Pengfei, Liu
Weiwei, Wang
Xiaofei, Ling
Qin, Lu
Jinwen, Zhang
Rui, He
Hang, Chen
author_sort Pengfei, Liu
collection PubMed
description Insect hormones regulate metamorphosis including that leading to sexual dimorphism. Using RNA-Seq, we discovered that the second-instar male larva (SM) of the white wax insect, Ericerus pela, have 5,968 and 8,620 differentially expressed transcripts compared with the second-instar female larva (SF) and the first-instar male larva (FM), respectively. The expression levels of genes involved in the apoptosis of old tissues and the reconstruction of new ones in the SM significantly enhanced, while the SF mainly has enhanced expression levels of anabolic genes such as chitin. We predicted that the second-instar larvae are the developmental origin of sexual dimorphic metamorphosis. Meanwhile, in the juvenile hormone (JH) metabolic pathway, CYP15A1 and JH esterase (JHE) are differentially expressed; and in the 20-hydroxyecdysone (20E) metabolic pathway, CYP307A1, CYP314A1, and CYP18A1 are differentially expressed. In the SM, the expression levels of CYP307A1 and CYP314A1 are significantly increased, whereas the expression level of CYP18A1 is significantly decreased; in the SF, the expression levels of the above genes are opposite to that of the SM. Expression trends of RNA-seq is consistent with the expression level of qRT–PCR, and seven of them are highly correlated (R ≥ 0.610) and four are moderately correlated (0.588 ≥ R ≥ 0.542).
format Online
Article
Text
id pubmed-6792091
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-67920912019-10-18 Regulation of Hormone-Related Genes in Ericerus pela (Hemiptera: Coccidae) for Dimorphic Metamorphosis Pengfei, Liu Weiwei, Wang Xiaofei, Ling Qin, Lu Jinwen, Zhang Rui, He Hang, Chen J Insect Sci Molecular Entomological Genetics Insect hormones regulate metamorphosis including that leading to sexual dimorphism. Using RNA-Seq, we discovered that the second-instar male larva (SM) of the white wax insect, Ericerus pela, have 5,968 and 8,620 differentially expressed transcripts compared with the second-instar female larva (SF) and the first-instar male larva (FM), respectively. The expression levels of genes involved in the apoptosis of old tissues and the reconstruction of new ones in the SM significantly enhanced, while the SF mainly has enhanced expression levels of anabolic genes such as chitin. We predicted that the second-instar larvae are the developmental origin of sexual dimorphic metamorphosis. Meanwhile, in the juvenile hormone (JH) metabolic pathway, CYP15A1 and JH esterase (JHE) are differentially expressed; and in the 20-hydroxyecdysone (20E) metabolic pathway, CYP307A1, CYP314A1, and CYP18A1 are differentially expressed. In the SM, the expression levels of CYP307A1 and CYP314A1 are significantly increased, whereas the expression level of CYP18A1 is significantly decreased; in the SF, the expression levels of the above genes are opposite to that of the SM. Expression trends of RNA-seq is consistent with the expression level of qRT–PCR, and seven of them are highly correlated (R ≥ 0.610) and four are moderately correlated (0.588 ≥ R ≥ 0.542). Oxford University Press 2019-10-14 /pmc/articles/PMC6792091/ /pubmed/31612946 http://dx.doi.org/10.1093/jisesa/iez092 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Entomological Society of America. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Molecular Entomological Genetics
Pengfei, Liu
Weiwei, Wang
Xiaofei, Ling
Qin, Lu
Jinwen, Zhang
Rui, He
Hang, Chen
Regulation of Hormone-Related Genes in Ericerus pela (Hemiptera: Coccidae) for Dimorphic Metamorphosis
title Regulation of Hormone-Related Genes in Ericerus pela (Hemiptera: Coccidae) for Dimorphic Metamorphosis
title_full Regulation of Hormone-Related Genes in Ericerus pela (Hemiptera: Coccidae) for Dimorphic Metamorphosis
title_fullStr Regulation of Hormone-Related Genes in Ericerus pela (Hemiptera: Coccidae) for Dimorphic Metamorphosis
title_full_unstemmed Regulation of Hormone-Related Genes in Ericerus pela (Hemiptera: Coccidae) for Dimorphic Metamorphosis
title_short Regulation of Hormone-Related Genes in Ericerus pela (Hemiptera: Coccidae) for Dimorphic Metamorphosis
title_sort regulation of hormone-related genes in ericerus pela (hemiptera: coccidae) for dimorphic metamorphosis
topic Molecular Entomological Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6792091/
https://www.ncbi.nlm.nih.gov/pubmed/31612946
http://dx.doi.org/10.1093/jisesa/iez092
work_keys_str_mv AT pengfeiliu regulationofhormonerelatedgenesinericeruspelahemipteracoccidaefordimorphicmetamorphosis
AT weiweiwang regulationofhormonerelatedgenesinericeruspelahemipteracoccidaefordimorphicmetamorphosis
AT xiaofeiling regulationofhormonerelatedgenesinericeruspelahemipteracoccidaefordimorphicmetamorphosis
AT qinlu regulationofhormonerelatedgenesinericeruspelahemipteracoccidaefordimorphicmetamorphosis
AT jinwenzhang regulationofhormonerelatedgenesinericeruspelahemipteracoccidaefordimorphicmetamorphosis
AT ruihe regulationofhormonerelatedgenesinericeruspelahemipteracoccidaefordimorphicmetamorphosis
AT hangchen regulationofhormonerelatedgenesinericeruspelahemipteracoccidaefordimorphicmetamorphosis