Cargando…

Evaluation of the immunogenicity and efficacy of BCG and MTBVAC vaccines using a natural transmission model of tuberculosis

Effective vaccines against tuberculosis (TB) are needed in order to prevent TB transmission in human and animal populations. Evaluation of TB vaccines may be facilitated by using reliable animal models that mimic host pathophysiology and natural transmission of the disease as closely as possible. In...

Descripción completa

Detalles Bibliográficos
Autores principales: Roy, Alvaro, Tomé, Irene, Romero, Beatriz, Lorente-Leal, Víctor, Infantes-Lorenzo, José A., Domínguez, Mercedes, Martín, Carlos, Aguiló, Nacho, Puentes, Eugenia, Rodríguez, Esteban, de Juan, Lucía, Risalde, María A., Gortázar, Christian, Domínguez, Lucas, Bezos, Javier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6792192/
https://www.ncbi.nlm.nih.gov/pubmed/31615555
http://dx.doi.org/10.1186/s13567-019-0702-7
_version_ 1783459095882235904
author Roy, Alvaro
Tomé, Irene
Romero, Beatriz
Lorente-Leal, Víctor
Infantes-Lorenzo, José A.
Domínguez, Mercedes
Martín, Carlos
Aguiló, Nacho
Puentes, Eugenia
Rodríguez, Esteban
de Juan, Lucía
Risalde, María A.
Gortázar, Christian
Domínguez, Lucas
Bezos, Javier
author_facet Roy, Alvaro
Tomé, Irene
Romero, Beatriz
Lorente-Leal, Víctor
Infantes-Lorenzo, José A.
Domínguez, Mercedes
Martín, Carlos
Aguiló, Nacho
Puentes, Eugenia
Rodríguez, Esteban
de Juan, Lucía
Risalde, María A.
Gortázar, Christian
Domínguez, Lucas
Bezos, Javier
author_sort Roy, Alvaro
collection PubMed
description Effective vaccines against tuberculosis (TB) are needed in order to prevent TB transmission in human and animal populations. Evaluation of TB vaccines may be facilitated by using reliable animal models that mimic host pathophysiology and natural transmission of the disease as closely as possible. In this study, we evaluated the immunogenicity and efficacy of two attenuated vaccines, BCG and MTBVAC, after each was given to 17 goats (2 months old) and then exposed for 9 months to goats infected with M. caprae. In general, MTBVAC-vaccinated goats showed higher interferon-gamma release than BCG vaccinated goats in response to bovine protein purified derivative and ESAT-6/CFP-10 antigens and the response was significantly higher than that observed in the control group until challenge. All animals showed lesions consistent with TB at the end of the study. Goats that received either vaccine showed significantly lower scores for pulmonary lymph nodes and total lesions than unvaccinated controls. Both MTBVAC and BCG vaccines proved to be immunogenic and effective in reducing severity of TB pathology caused by M. caprae. Our model system of natural TB transmission may be useful for evaluating and optimizing vaccines.
format Online
Article
Text
id pubmed-6792192
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-67921922019-10-21 Evaluation of the immunogenicity and efficacy of BCG and MTBVAC vaccines using a natural transmission model of tuberculosis Roy, Alvaro Tomé, Irene Romero, Beatriz Lorente-Leal, Víctor Infantes-Lorenzo, José A. Domínguez, Mercedes Martín, Carlos Aguiló, Nacho Puentes, Eugenia Rodríguez, Esteban de Juan, Lucía Risalde, María A. Gortázar, Christian Domínguez, Lucas Bezos, Javier Vet Res Research Article Effective vaccines against tuberculosis (TB) are needed in order to prevent TB transmission in human and animal populations. Evaluation of TB vaccines may be facilitated by using reliable animal models that mimic host pathophysiology and natural transmission of the disease as closely as possible. In this study, we evaluated the immunogenicity and efficacy of two attenuated vaccines, BCG and MTBVAC, after each was given to 17 goats (2 months old) and then exposed for 9 months to goats infected with M. caprae. In general, MTBVAC-vaccinated goats showed higher interferon-gamma release than BCG vaccinated goats in response to bovine protein purified derivative and ESAT-6/CFP-10 antigens and the response was significantly higher than that observed in the control group until challenge. All animals showed lesions consistent with TB at the end of the study. Goats that received either vaccine showed significantly lower scores for pulmonary lymph nodes and total lesions than unvaccinated controls. Both MTBVAC and BCG vaccines proved to be immunogenic and effective in reducing severity of TB pathology caused by M. caprae. Our model system of natural TB transmission may be useful for evaluating and optimizing vaccines. BioMed Central 2019-10-15 2019 /pmc/articles/PMC6792192/ /pubmed/31615555 http://dx.doi.org/10.1186/s13567-019-0702-7 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Roy, Alvaro
Tomé, Irene
Romero, Beatriz
Lorente-Leal, Víctor
Infantes-Lorenzo, José A.
Domínguez, Mercedes
Martín, Carlos
Aguiló, Nacho
Puentes, Eugenia
Rodríguez, Esteban
de Juan, Lucía
Risalde, María A.
Gortázar, Christian
Domínguez, Lucas
Bezos, Javier
Evaluation of the immunogenicity and efficacy of BCG and MTBVAC vaccines using a natural transmission model of tuberculosis
title Evaluation of the immunogenicity and efficacy of BCG and MTBVAC vaccines using a natural transmission model of tuberculosis
title_full Evaluation of the immunogenicity and efficacy of BCG and MTBVAC vaccines using a natural transmission model of tuberculosis
title_fullStr Evaluation of the immunogenicity and efficacy of BCG and MTBVAC vaccines using a natural transmission model of tuberculosis
title_full_unstemmed Evaluation of the immunogenicity and efficacy of BCG and MTBVAC vaccines using a natural transmission model of tuberculosis
title_short Evaluation of the immunogenicity and efficacy of BCG and MTBVAC vaccines using a natural transmission model of tuberculosis
title_sort evaluation of the immunogenicity and efficacy of bcg and mtbvac vaccines using a natural transmission model of tuberculosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6792192/
https://www.ncbi.nlm.nih.gov/pubmed/31615555
http://dx.doi.org/10.1186/s13567-019-0702-7
work_keys_str_mv AT royalvaro evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis
AT tomeirene evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis
AT romerobeatriz evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis
AT lorentelealvictor evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis
AT infanteslorenzojosea evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis
AT dominguezmercedes evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis
AT martincarlos evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis
AT aguilonacho evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis
AT puenteseugenia evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis
AT rodriguezesteban evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis
AT dejuanlucia evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis
AT risaldemariaa evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis
AT gortazarchristian evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis
AT dominguezlucas evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis
AT bezosjavier evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis