Cargando…
Evaluation of the immunogenicity and efficacy of BCG and MTBVAC vaccines using a natural transmission model of tuberculosis
Effective vaccines against tuberculosis (TB) are needed in order to prevent TB transmission in human and animal populations. Evaluation of TB vaccines may be facilitated by using reliable animal models that mimic host pathophysiology and natural transmission of the disease as closely as possible. In...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6792192/ https://www.ncbi.nlm.nih.gov/pubmed/31615555 http://dx.doi.org/10.1186/s13567-019-0702-7 |
_version_ | 1783459095882235904 |
---|---|
author | Roy, Alvaro Tomé, Irene Romero, Beatriz Lorente-Leal, Víctor Infantes-Lorenzo, José A. Domínguez, Mercedes Martín, Carlos Aguiló, Nacho Puentes, Eugenia Rodríguez, Esteban de Juan, Lucía Risalde, María A. Gortázar, Christian Domínguez, Lucas Bezos, Javier |
author_facet | Roy, Alvaro Tomé, Irene Romero, Beatriz Lorente-Leal, Víctor Infantes-Lorenzo, José A. Domínguez, Mercedes Martín, Carlos Aguiló, Nacho Puentes, Eugenia Rodríguez, Esteban de Juan, Lucía Risalde, María A. Gortázar, Christian Domínguez, Lucas Bezos, Javier |
author_sort | Roy, Alvaro |
collection | PubMed |
description | Effective vaccines against tuberculosis (TB) are needed in order to prevent TB transmission in human and animal populations. Evaluation of TB vaccines may be facilitated by using reliable animal models that mimic host pathophysiology and natural transmission of the disease as closely as possible. In this study, we evaluated the immunogenicity and efficacy of two attenuated vaccines, BCG and MTBVAC, after each was given to 17 goats (2 months old) and then exposed for 9 months to goats infected with M. caprae. In general, MTBVAC-vaccinated goats showed higher interferon-gamma release than BCG vaccinated goats in response to bovine protein purified derivative and ESAT-6/CFP-10 antigens and the response was significantly higher than that observed in the control group until challenge. All animals showed lesions consistent with TB at the end of the study. Goats that received either vaccine showed significantly lower scores for pulmonary lymph nodes and total lesions than unvaccinated controls. Both MTBVAC and BCG vaccines proved to be immunogenic and effective in reducing severity of TB pathology caused by M. caprae. Our model system of natural TB transmission may be useful for evaluating and optimizing vaccines. |
format | Online Article Text |
id | pubmed-6792192 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-67921922019-10-21 Evaluation of the immunogenicity and efficacy of BCG and MTBVAC vaccines using a natural transmission model of tuberculosis Roy, Alvaro Tomé, Irene Romero, Beatriz Lorente-Leal, Víctor Infantes-Lorenzo, José A. Domínguez, Mercedes Martín, Carlos Aguiló, Nacho Puentes, Eugenia Rodríguez, Esteban de Juan, Lucía Risalde, María A. Gortázar, Christian Domínguez, Lucas Bezos, Javier Vet Res Research Article Effective vaccines against tuberculosis (TB) are needed in order to prevent TB transmission in human and animal populations. Evaluation of TB vaccines may be facilitated by using reliable animal models that mimic host pathophysiology and natural transmission of the disease as closely as possible. In this study, we evaluated the immunogenicity and efficacy of two attenuated vaccines, BCG and MTBVAC, after each was given to 17 goats (2 months old) and then exposed for 9 months to goats infected with M. caprae. In general, MTBVAC-vaccinated goats showed higher interferon-gamma release than BCG vaccinated goats in response to bovine protein purified derivative and ESAT-6/CFP-10 antigens and the response was significantly higher than that observed in the control group until challenge. All animals showed lesions consistent with TB at the end of the study. Goats that received either vaccine showed significantly lower scores for pulmonary lymph nodes and total lesions than unvaccinated controls. Both MTBVAC and BCG vaccines proved to be immunogenic and effective in reducing severity of TB pathology caused by M. caprae. Our model system of natural TB transmission may be useful for evaluating and optimizing vaccines. BioMed Central 2019-10-15 2019 /pmc/articles/PMC6792192/ /pubmed/31615555 http://dx.doi.org/10.1186/s13567-019-0702-7 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Roy, Alvaro Tomé, Irene Romero, Beatriz Lorente-Leal, Víctor Infantes-Lorenzo, José A. Domínguez, Mercedes Martín, Carlos Aguiló, Nacho Puentes, Eugenia Rodríguez, Esteban de Juan, Lucía Risalde, María A. Gortázar, Christian Domínguez, Lucas Bezos, Javier Evaluation of the immunogenicity and efficacy of BCG and MTBVAC vaccines using a natural transmission model of tuberculosis |
title | Evaluation of the immunogenicity and efficacy of BCG and MTBVAC vaccines using a natural transmission model of tuberculosis |
title_full | Evaluation of the immunogenicity and efficacy of BCG and MTBVAC vaccines using a natural transmission model of tuberculosis |
title_fullStr | Evaluation of the immunogenicity and efficacy of BCG and MTBVAC vaccines using a natural transmission model of tuberculosis |
title_full_unstemmed | Evaluation of the immunogenicity and efficacy of BCG and MTBVAC vaccines using a natural transmission model of tuberculosis |
title_short | Evaluation of the immunogenicity and efficacy of BCG and MTBVAC vaccines using a natural transmission model of tuberculosis |
title_sort | evaluation of the immunogenicity and efficacy of bcg and mtbvac vaccines using a natural transmission model of tuberculosis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6792192/ https://www.ncbi.nlm.nih.gov/pubmed/31615555 http://dx.doi.org/10.1186/s13567-019-0702-7 |
work_keys_str_mv | AT royalvaro evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis AT tomeirene evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis AT romerobeatriz evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis AT lorentelealvictor evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis AT infanteslorenzojosea evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis AT dominguezmercedes evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis AT martincarlos evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis AT aguilonacho evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis AT puenteseugenia evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis AT rodriguezesteban evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis AT dejuanlucia evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis AT risaldemariaa evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis AT gortazarchristian evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis AT dominguezlucas evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis AT bezosjavier evaluationoftheimmunogenicityandefficacyofbcgandmtbvacvaccinesusinganaturaltransmissionmodeloftuberculosis |