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miRNA-339-5p Plays an Important Role in Invasion and Migration of Pancreatic Cancer Cells

BACKGROUND: This study aimed to investigate the role of miRNA-339-5p in pancreatic cancer cell invasion and migration. MATERIAL/METHODS: The differences between exosomal miRNAs of PANC02 and PANC02-H7 were studied by microarray analysis. We measured miRNA-339-5p expression in different groups; diffe...

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Autores principales: Yu, Zeqian, Zhao, Susu, Wang, Lishan, Wang, Junying, Zhou, Jiahua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6792519/
https://www.ncbi.nlm.nih.gov/pubmed/31588120
http://dx.doi.org/10.12659/MSM.917038
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author Yu, Zeqian
Zhao, Susu
Wang, Lishan
Wang, Junying
Zhou, Jiahua
author_facet Yu, Zeqian
Zhao, Susu
Wang, Lishan
Wang, Junying
Zhou, Jiahua
author_sort Yu, Zeqian
collection PubMed
description BACKGROUND: This study aimed to investigate the role of miRNA-339-5p in pancreatic cancer cell invasion and migration. MATERIAL/METHODS: The differences between exosomal miRNAs of PANC02 and PANC02-H7 were studied by microarray analysis. We measured miRNA-339-5p expression in different groups; differences in cell invasion and migration were evaluated using the Transwell and wound healing assays and expression of relative proteins (E-cadherin, vimentin and ZNF689) was measured by WB assay. The correlation between miRNA-339-5p and ZNF689 expression was evaluated by luciferase reporter gene assay. RESULTS: Compared with PANC02 exosome, microarray analysis indicated that miRNA-339-5p mRNA expression was significantly suppressed (P<0.001) in the PANC02-H7 exosome. Supplementation with miR-339-5p mimics led to a significant decrease in the invasion cell number and wound healing rate (P<0.001), with significantly enhanced E-cadherin expression and suppressed vimentin expression (P<0.001). However, transfection of a miR-339-5p inhibitor led to a significant increase in the invasion cell number and wound healing rate (P<0.001), with significantly suppressed E-cadherin expression and increased vimentin expression (P<0.001). Luciferase reporter gene assay demonstrated ZNF689 gene to be the target of miR-339-5p in the PANC02-H7 cell. With miR-339-5p and ZNF689 transfection, the invasion cell number and wound healing rate were significantly increased compared with those in the miR-339-5p group (P<0.001), with significantly increased expression of ZNF689 and vimentin and suppressed E-cadherin expression (P<0.001). CONCLUSIONS: miR-339-5p suppresses the invasion and migration of pancreatic cancer cells via direct regulation of ZNF689 in vitro.
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spelling pubmed-67925192019-10-31 miRNA-339-5p Plays an Important Role in Invasion and Migration of Pancreatic Cancer Cells Yu, Zeqian Zhao, Susu Wang, Lishan Wang, Junying Zhou, Jiahua Med Sci Monit Lab/In Vitro Research BACKGROUND: This study aimed to investigate the role of miRNA-339-5p in pancreatic cancer cell invasion and migration. MATERIAL/METHODS: The differences between exosomal miRNAs of PANC02 and PANC02-H7 were studied by microarray analysis. We measured miRNA-339-5p expression in different groups; differences in cell invasion and migration were evaluated using the Transwell and wound healing assays and expression of relative proteins (E-cadherin, vimentin and ZNF689) was measured by WB assay. The correlation between miRNA-339-5p and ZNF689 expression was evaluated by luciferase reporter gene assay. RESULTS: Compared with PANC02 exosome, microarray analysis indicated that miRNA-339-5p mRNA expression was significantly suppressed (P<0.001) in the PANC02-H7 exosome. Supplementation with miR-339-5p mimics led to a significant decrease in the invasion cell number and wound healing rate (P<0.001), with significantly enhanced E-cadherin expression and suppressed vimentin expression (P<0.001). However, transfection of a miR-339-5p inhibitor led to a significant increase in the invasion cell number and wound healing rate (P<0.001), with significantly suppressed E-cadherin expression and increased vimentin expression (P<0.001). Luciferase reporter gene assay demonstrated ZNF689 gene to be the target of miR-339-5p in the PANC02-H7 cell. With miR-339-5p and ZNF689 transfection, the invasion cell number and wound healing rate were significantly increased compared with those in the miR-339-5p group (P<0.001), with significantly increased expression of ZNF689 and vimentin and suppressed E-cadherin expression (P<0.001). CONCLUSIONS: miR-339-5p suppresses the invasion and migration of pancreatic cancer cells via direct regulation of ZNF689 in vitro. International Scientific Literature, Inc. 2019-10-07 /pmc/articles/PMC6792519/ /pubmed/31588120 http://dx.doi.org/10.12659/MSM.917038 Text en © Med Sci Monit, 2019 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) )
spellingShingle Lab/In Vitro Research
Yu, Zeqian
Zhao, Susu
Wang, Lishan
Wang, Junying
Zhou, Jiahua
miRNA-339-5p Plays an Important Role in Invasion and Migration of Pancreatic Cancer Cells
title miRNA-339-5p Plays an Important Role in Invasion and Migration of Pancreatic Cancer Cells
title_full miRNA-339-5p Plays an Important Role in Invasion and Migration of Pancreatic Cancer Cells
title_fullStr miRNA-339-5p Plays an Important Role in Invasion and Migration of Pancreatic Cancer Cells
title_full_unstemmed miRNA-339-5p Plays an Important Role in Invasion and Migration of Pancreatic Cancer Cells
title_short miRNA-339-5p Plays an Important Role in Invasion and Migration of Pancreatic Cancer Cells
title_sort mirna-339-5p plays an important role in invasion and migration of pancreatic cancer cells
topic Lab/In Vitro Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6792519/
https://www.ncbi.nlm.nih.gov/pubmed/31588120
http://dx.doi.org/10.12659/MSM.917038
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