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Protease-associated import systems are widespread in Gram-negative bacteria

Bacteria have evolved sophisticated uptake machineries in order to obtain the nutrients required for growth. Gram-negative plant pathogens of the genus Pectobacterium obtain iron from the protein ferredoxin, which is produced by their plant hosts. This iron-piracy is mediated by the ferredoxin uptak...

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Autores principales: Grinter, Rhys, Leung, Pok Man, Wijeyewickrema, Lakshmi C., Littler, Dene, Beckham, Simone, Pike, Robert N., Walker, Daniel, Greening, Chris, Lithgow, Trevor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6793856/
https://www.ncbi.nlm.nih.gov/pubmed/31613892
http://dx.doi.org/10.1371/journal.pgen.1008435
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author Grinter, Rhys
Leung, Pok Man
Wijeyewickrema, Lakshmi C.
Littler, Dene
Beckham, Simone
Pike, Robert N.
Walker, Daniel
Greening, Chris
Lithgow, Trevor
author_facet Grinter, Rhys
Leung, Pok Man
Wijeyewickrema, Lakshmi C.
Littler, Dene
Beckham, Simone
Pike, Robert N.
Walker, Daniel
Greening, Chris
Lithgow, Trevor
author_sort Grinter, Rhys
collection PubMed
description Bacteria have evolved sophisticated uptake machineries in order to obtain the nutrients required for growth. Gram-negative plant pathogens of the genus Pectobacterium obtain iron from the protein ferredoxin, which is produced by their plant hosts. This iron-piracy is mediated by the ferredoxin uptake system (Fus), a gene cluster encoding proteins that transport ferredoxin into the bacterial cell and process it proteolytically. In this work we show that gene clusters related to the Fus are widespread in bacterial species. Through structural and biochemical characterisation of the distantly related Fus homologues YddB and PqqL from Escherichia coli, we show that these proteins are analogous to components of the Fus from Pectobacterium. The membrane protein YddB shares common structural features with the outer membrane ferredoxin transporter FusA, including a large extracellular substrate binding site. PqqL is an active protease with an analogous periplasmic localisation and iron-dependent expression to the ferredoxin processing protease FusC. Structural analysis demonstrates that PqqL and FusC share specific features that distinguish them from other members of the M16 protease family. Taken together, these data provide evidence that protease associated import systems analogous to the Fus are widespread in Gram-negative bacteria.
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spelling pubmed-67938562019-10-25 Protease-associated import systems are widespread in Gram-negative bacteria Grinter, Rhys Leung, Pok Man Wijeyewickrema, Lakshmi C. Littler, Dene Beckham, Simone Pike, Robert N. Walker, Daniel Greening, Chris Lithgow, Trevor PLoS Genet Research Article Bacteria have evolved sophisticated uptake machineries in order to obtain the nutrients required for growth. Gram-negative plant pathogens of the genus Pectobacterium obtain iron from the protein ferredoxin, which is produced by their plant hosts. This iron-piracy is mediated by the ferredoxin uptake system (Fus), a gene cluster encoding proteins that transport ferredoxin into the bacterial cell and process it proteolytically. In this work we show that gene clusters related to the Fus are widespread in bacterial species. Through structural and biochemical characterisation of the distantly related Fus homologues YddB and PqqL from Escherichia coli, we show that these proteins are analogous to components of the Fus from Pectobacterium. The membrane protein YddB shares common structural features with the outer membrane ferredoxin transporter FusA, including a large extracellular substrate binding site. PqqL is an active protease with an analogous periplasmic localisation and iron-dependent expression to the ferredoxin processing protease FusC. Structural analysis demonstrates that PqqL and FusC share specific features that distinguish them from other members of the M16 protease family. Taken together, these data provide evidence that protease associated import systems analogous to the Fus are widespread in Gram-negative bacteria. Public Library of Science 2019-10-15 /pmc/articles/PMC6793856/ /pubmed/31613892 http://dx.doi.org/10.1371/journal.pgen.1008435 Text en © 2019 Grinter et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Grinter, Rhys
Leung, Pok Man
Wijeyewickrema, Lakshmi C.
Littler, Dene
Beckham, Simone
Pike, Robert N.
Walker, Daniel
Greening, Chris
Lithgow, Trevor
Protease-associated import systems are widespread in Gram-negative bacteria
title Protease-associated import systems are widespread in Gram-negative bacteria
title_full Protease-associated import systems are widespread in Gram-negative bacteria
title_fullStr Protease-associated import systems are widespread in Gram-negative bacteria
title_full_unstemmed Protease-associated import systems are widespread in Gram-negative bacteria
title_short Protease-associated import systems are widespread in Gram-negative bacteria
title_sort protease-associated import systems are widespread in gram-negative bacteria
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6793856/
https://www.ncbi.nlm.nih.gov/pubmed/31613892
http://dx.doi.org/10.1371/journal.pgen.1008435
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