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Phosphorus-mediated alleviation of aluminum toxicity revealed by the iTRAQ technique in Citrus grandis roots

Citrus grandis seedlings were irrigated with nutrient solutions with four Al-P combinations [two Al levels (0 mM and 1.2 mM AlCl(3)·6H(2)O) × two P levels (0 μM and 200 μM KH(2)PO(4))] for 18 weeks. Al dramatically inhibited the growth of C. grandis seedlings, as revealed by a decreased dry weight o...

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Autores principales: Yang, Lin-Tong, Zhou, Yang-Fei, Wang, Yan-Yu, Wu, Yan-Mei, Qian, Bing, Wang, Heng, Chen, Li-Song
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6793874/
https://www.ncbi.nlm.nih.gov/pubmed/31613915
http://dx.doi.org/10.1371/journal.pone.0223516
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author Yang, Lin-Tong
Zhou, Yang-Fei
Wang, Yan-Yu
Wu, Yan-Mei
Qian, Bing
Wang, Heng
Chen, Li-Song
author_facet Yang, Lin-Tong
Zhou, Yang-Fei
Wang, Yan-Yu
Wu, Yan-Mei
Qian, Bing
Wang, Heng
Chen, Li-Song
author_sort Yang, Lin-Tong
collection PubMed
description Citrus grandis seedlings were irrigated with nutrient solutions with four Al-P combinations [two Al levels (0 mM and 1.2 mM AlCl(3)·6H(2)O) × two P levels (0 μM and 200 μM KH(2)PO(4))] for 18 weeks. Al dramatically inhibited the growth of C. grandis seedlings, as revealed by a decreased dry weight of roots and shoots. Elevating P level could ameliorate the Al-induced growth inhibition and organic acid (malate and citrate) secretion in C. grandis. Using a comparative proteomic approach revealed by the isobaric tags for relative and absolute quantification (iTRAQ) technique, 318 differentially abundant proteins (DAPs) were successfully identified and quantified in this study. The possible mechanisms underlying P-induced alleviation of Al toxicity in C. grandis were proposed. Furthermore, some DAPs, such as GLN phosphoribosyl pyrophosphate amidotransferase 2, ATP-dependent caseinolytic (Clp) protease/crotonase family protein, methionine-S-oxide reductase B2, ABC transporter I family member 17 and pyridoxal phosphate phosphatase, were reported for the first time to respond to Al stress in Citrus plants. Our study provides some proteomic details about the alleviative effects of P on Al toxicity in C. grandis, however, the exact function of the DAPs identified herein in response to Al tolerance in plants must be further investigated.
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spelling pubmed-67938742019-10-25 Phosphorus-mediated alleviation of aluminum toxicity revealed by the iTRAQ technique in Citrus grandis roots Yang, Lin-Tong Zhou, Yang-Fei Wang, Yan-Yu Wu, Yan-Mei Qian, Bing Wang, Heng Chen, Li-Song PLoS One Research Article Citrus grandis seedlings were irrigated with nutrient solutions with four Al-P combinations [two Al levels (0 mM and 1.2 mM AlCl(3)·6H(2)O) × two P levels (0 μM and 200 μM KH(2)PO(4))] for 18 weeks. Al dramatically inhibited the growth of C. grandis seedlings, as revealed by a decreased dry weight of roots and shoots. Elevating P level could ameliorate the Al-induced growth inhibition and organic acid (malate and citrate) secretion in C. grandis. Using a comparative proteomic approach revealed by the isobaric tags for relative and absolute quantification (iTRAQ) technique, 318 differentially abundant proteins (DAPs) were successfully identified and quantified in this study. The possible mechanisms underlying P-induced alleviation of Al toxicity in C. grandis were proposed. Furthermore, some DAPs, such as GLN phosphoribosyl pyrophosphate amidotransferase 2, ATP-dependent caseinolytic (Clp) protease/crotonase family protein, methionine-S-oxide reductase B2, ABC transporter I family member 17 and pyridoxal phosphate phosphatase, were reported for the first time to respond to Al stress in Citrus plants. Our study provides some proteomic details about the alleviative effects of P on Al toxicity in C. grandis, however, the exact function of the DAPs identified herein in response to Al tolerance in plants must be further investigated. Public Library of Science 2019-10-15 /pmc/articles/PMC6793874/ /pubmed/31613915 http://dx.doi.org/10.1371/journal.pone.0223516 Text en © 2019 Yang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Yang, Lin-Tong
Zhou, Yang-Fei
Wang, Yan-Yu
Wu, Yan-Mei
Qian, Bing
Wang, Heng
Chen, Li-Song
Phosphorus-mediated alleviation of aluminum toxicity revealed by the iTRAQ technique in Citrus grandis roots
title Phosphorus-mediated alleviation of aluminum toxicity revealed by the iTRAQ technique in Citrus grandis roots
title_full Phosphorus-mediated alleviation of aluminum toxicity revealed by the iTRAQ technique in Citrus grandis roots
title_fullStr Phosphorus-mediated alleviation of aluminum toxicity revealed by the iTRAQ technique in Citrus grandis roots
title_full_unstemmed Phosphorus-mediated alleviation of aluminum toxicity revealed by the iTRAQ technique in Citrus grandis roots
title_short Phosphorus-mediated alleviation of aluminum toxicity revealed by the iTRAQ technique in Citrus grandis roots
title_sort phosphorus-mediated alleviation of aluminum toxicity revealed by the itraq technique in citrus grandis roots
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6793874/
https://www.ncbi.nlm.nih.gov/pubmed/31613915
http://dx.doi.org/10.1371/journal.pone.0223516
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