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Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicity
Animal circadian rhythms persist in constant darkness and are driven by intracellular transcription-translation feedback loops. Although these cellular oscillators communicate, isolated mammalian cellular clocks continue to tick away in darkness without intercellular communication. To investigate th...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6794074/ https://www.ncbi.nlm.nih.gov/pubmed/31613223 http://dx.doi.org/10.7554/eLife.48301 |
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author | Schlichting, Matthias Díaz, Madelen M Xin, Jason Rosbash, Michael |
author_facet | Schlichting, Matthias Díaz, Madelen M Xin, Jason Rosbash, Michael |
author_sort | Schlichting, Matthias |
collection | PubMed |
description | Animal circadian rhythms persist in constant darkness and are driven by intracellular transcription-translation feedback loops. Although these cellular oscillators communicate, isolated mammalian cellular clocks continue to tick away in darkness without intercellular communication. To investigate these issues in Drosophila, we assayed behavior as well as molecular rhythms within individual brain clock neurons while blocking communication within the ca. 150 neuron clock network. We also generated CRISPR-mediated neuron-specific circadian clock knockouts. The results point to two key clock neuron groups: loss of the clock within both regions but neither one alone has a strong behavioral phenotype in darkness; communication between these regions also contributes to circadian period determination. Under these dark conditions, the clock within one region persists without network communication. The clock within the famous PDF-expressing s-LNv neurons however was strongly dependent on network communication, likely because clock gene expression within these vulnerable sLNvs depends on neuronal firing or light. |
format | Online Article Text |
id | pubmed-6794074 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-67940742019-10-16 Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicity Schlichting, Matthias Díaz, Madelen M Xin, Jason Rosbash, Michael eLife Neuroscience Animal circadian rhythms persist in constant darkness and are driven by intracellular transcription-translation feedback loops. Although these cellular oscillators communicate, isolated mammalian cellular clocks continue to tick away in darkness without intercellular communication. To investigate these issues in Drosophila, we assayed behavior as well as molecular rhythms within individual brain clock neurons while blocking communication within the ca. 150 neuron clock network. We also generated CRISPR-mediated neuron-specific circadian clock knockouts. The results point to two key clock neuron groups: loss of the clock within both regions but neither one alone has a strong behavioral phenotype in darkness; communication between these regions also contributes to circadian period determination. Under these dark conditions, the clock within one region persists without network communication. The clock within the famous PDF-expressing s-LNv neurons however was strongly dependent on network communication, likely because clock gene expression within these vulnerable sLNvs depends on neuronal firing or light. eLife Sciences Publications, Ltd 2019-10-15 /pmc/articles/PMC6794074/ /pubmed/31613223 http://dx.doi.org/10.7554/eLife.48301 Text en © 2019, Schlichting et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Schlichting, Matthias Díaz, Madelen M Xin, Jason Rosbash, Michael Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicity |
title | Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicity |
title_full | Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicity |
title_fullStr | Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicity |
title_full_unstemmed | Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicity |
title_short | Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicity |
title_sort | neuron-specific knockouts indicate the importance of network communication to drosophila rhythmicity |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6794074/ https://www.ncbi.nlm.nih.gov/pubmed/31613223 http://dx.doi.org/10.7554/eLife.48301 |
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