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Role of Atg8 in the regulation of vacuolar membrane invagination

Cellular heat stress can cause damage, and significant changes, to a variety of cellular structures. When exposed to chronically high temperatures, yeast cells invaginate vacuolar membranes. In this study, we found that the expression of Atg8, an essential autophagy factor, is induced after chronic...

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Autores principales: Ishii, Ayane, Kurokawa, Kazuo, Hotta, Miyuu, Yoshizaki, Suzuka, Kurita, Maki, Koyama, Aya, Nakano, Akihiko, Kimura, Yoko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6794316/
https://www.ncbi.nlm.nih.gov/pubmed/31616012
http://dx.doi.org/10.1038/s41598-019-51254-1
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author Ishii, Ayane
Kurokawa, Kazuo
Hotta, Miyuu
Yoshizaki, Suzuka
Kurita, Maki
Koyama, Aya
Nakano, Akihiko
Kimura, Yoko
author_facet Ishii, Ayane
Kurokawa, Kazuo
Hotta, Miyuu
Yoshizaki, Suzuka
Kurita, Maki
Koyama, Aya
Nakano, Akihiko
Kimura, Yoko
author_sort Ishii, Ayane
collection PubMed
description Cellular heat stress can cause damage, and significant changes, to a variety of cellular structures. When exposed to chronically high temperatures, yeast cells invaginate vacuolar membranes. In this study, we found that the expression of Atg8, an essential autophagy factor, is induced after chronic heat stress. In addition, without Atg8, vacuolar invaginations are induced conspicuously, beginning earlier and invaginating vacuoles more frequently after heat stress. Our results indicate that Atg8’s invagination-suppressing functions do not require Atg8 lipidation, in contrast with autophagy, which requires Atg8 lipidation. Genetic analyses of vps24 and vps23 further suggest that full ESCRT machinery is necessary to form vacuolar invaginations irrespective of Atg8. In contrast, through a combined mutation with the vacuole BAR domain protein Ivy1, vacuoles show constitutively enhanced invaginated structures. Finally, we found that the atg8Δivy1Δ mutant is sensitive against agents targeting functions of the vacuole and/or plasma membrane (cell wall). Collectively, our findings revealed that Atg8 maintains vacuolar membrane homeostasis in an autophagy-independent function by coordinating with other cellular factors.
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spelling pubmed-67943162019-10-25 Role of Atg8 in the regulation of vacuolar membrane invagination Ishii, Ayane Kurokawa, Kazuo Hotta, Miyuu Yoshizaki, Suzuka Kurita, Maki Koyama, Aya Nakano, Akihiko Kimura, Yoko Sci Rep Article Cellular heat stress can cause damage, and significant changes, to a variety of cellular structures. When exposed to chronically high temperatures, yeast cells invaginate vacuolar membranes. In this study, we found that the expression of Atg8, an essential autophagy factor, is induced after chronic heat stress. In addition, without Atg8, vacuolar invaginations are induced conspicuously, beginning earlier and invaginating vacuoles more frequently after heat stress. Our results indicate that Atg8’s invagination-suppressing functions do not require Atg8 lipidation, in contrast with autophagy, which requires Atg8 lipidation. Genetic analyses of vps24 and vps23 further suggest that full ESCRT machinery is necessary to form vacuolar invaginations irrespective of Atg8. In contrast, through a combined mutation with the vacuole BAR domain protein Ivy1, vacuoles show constitutively enhanced invaginated structures. Finally, we found that the atg8Δivy1Δ mutant is sensitive against agents targeting functions of the vacuole and/or plasma membrane (cell wall). Collectively, our findings revealed that Atg8 maintains vacuolar membrane homeostasis in an autophagy-independent function by coordinating with other cellular factors. Nature Publishing Group UK 2019-10-15 /pmc/articles/PMC6794316/ /pubmed/31616012 http://dx.doi.org/10.1038/s41598-019-51254-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ishii, Ayane
Kurokawa, Kazuo
Hotta, Miyuu
Yoshizaki, Suzuka
Kurita, Maki
Koyama, Aya
Nakano, Akihiko
Kimura, Yoko
Role of Atg8 in the regulation of vacuolar membrane invagination
title Role of Atg8 in the regulation of vacuolar membrane invagination
title_full Role of Atg8 in the regulation of vacuolar membrane invagination
title_fullStr Role of Atg8 in the regulation of vacuolar membrane invagination
title_full_unstemmed Role of Atg8 in the regulation of vacuolar membrane invagination
title_short Role of Atg8 in the regulation of vacuolar membrane invagination
title_sort role of atg8 in the regulation of vacuolar membrane invagination
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6794316/
https://www.ncbi.nlm.nih.gov/pubmed/31616012
http://dx.doi.org/10.1038/s41598-019-51254-1
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