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Optimized Protocol for the Detection of Multifunctional Epitope-Specific CD4(+) T Cells Combining MHC-II Tetramer and Intracellular Cytokine Staining Technologies
Analysis of multifunctional CD4(+) T cells is fundamental for characterizing the immune responses to vaccination or infection. Major histocompatibility complex (MHC)/peptide tetramers represent a powerful technology for the detection of antigen-specific T cells by specific binding to their T-cell re...
Autores principales: | Pastore, Gabiria, Carraro, Monica, Pettini, Elena, Nolfi, Emanuele, Medaglini, Donata, Ciabattini, Annalisa |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6794358/ https://www.ncbi.nlm.nih.gov/pubmed/31649661 http://dx.doi.org/10.3389/fimmu.2019.02304 |
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