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Comprehensive Transcriptomic Analysis of Mouse Gonadal Development Involving Sexual Differentiation, Meiosis and Gametogenesis

BACKGROUND: Mammalian gonadal development is crucial for fertility. Sexual differentiation, meiosis and gametogenesis are critical events in the process of gonadal development. Abnormalities in any of these events may cause infertility. However, owing to the complexity of these developmental events,...

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Autores principales: Wang, Jian, Tian, Geng G., Zheng, Zhuxia, Li, Bo, Xing, Qinghe, Wu, Ji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6794783/
https://www.ncbi.nlm.nih.gov/pubmed/31636514
http://dx.doi.org/10.1186/s12575-019-0108-y
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author Wang, Jian
Tian, Geng G.
Zheng, Zhuxia
Li, Bo
Xing, Qinghe
Wu, Ji
author_facet Wang, Jian
Tian, Geng G.
Zheng, Zhuxia
Li, Bo
Xing, Qinghe
Wu, Ji
author_sort Wang, Jian
collection PubMed
description BACKGROUND: Mammalian gonadal development is crucial for fertility. Sexual differentiation, meiosis and gametogenesis are critical events in the process of gonadal development. Abnormalities in any of these events may cause infertility. However, owing to the complexity of these developmental events, the underlying molecular mechanisms are not fully understood and require further research. RESULTS: In this study, we employed RNA sequencing to examine transcriptome profiles of murine female and male gonads at crucial stages of these developmental events. By bioinformatics analysis, we identified a group of candidate genes that may participate in sexual differentiation, including Erbb3, Erbb4, and Prkg2. One hundred and two and 134 candidate genes that may be important for female and male gonadal development, respectively, were screened by analyzing the global gene expression patterns of developing female and male gonads. Weighted gene co-expression network analysis was performed on developing female gonads, and we identified a gene co-expression module related to meiosis. By alternative splicing analysis, we found that cassette-type exon and alternative start sites were the main forms of alternative splicing in developing gonads. A considerable portion of differentially expressed and alternatively spliced genes were involved in meiosis. CONCLUSION: Taken together, our findings have enriched the gonadal transcriptome database and provided novel candidate genes and avenues to research the molecular mechanisms of sexual differentiation, meiosis, and gametogenesis. SUPPLEMENTARY INFORMATION: Supplementary information accompanies this paper at 10.1186/s12575-019-0108-y.
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spelling pubmed-67947832019-10-21 Comprehensive Transcriptomic Analysis of Mouse Gonadal Development Involving Sexual Differentiation, Meiosis and Gametogenesis Wang, Jian Tian, Geng G. Zheng, Zhuxia Li, Bo Xing, Qinghe Wu, Ji Biol Proced Online Research BACKGROUND: Mammalian gonadal development is crucial for fertility. Sexual differentiation, meiosis and gametogenesis are critical events in the process of gonadal development. Abnormalities in any of these events may cause infertility. However, owing to the complexity of these developmental events, the underlying molecular mechanisms are not fully understood and require further research. RESULTS: In this study, we employed RNA sequencing to examine transcriptome profiles of murine female and male gonads at crucial stages of these developmental events. By bioinformatics analysis, we identified a group of candidate genes that may participate in sexual differentiation, including Erbb3, Erbb4, and Prkg2. One hundred and two and 134 candidate genes that may be important for female and male gonadal development, respectively, were screened by analyzing the global gene expression patterns of developing female and male gonads. Weighted gene co-expression network analysis was performed on developing female gonads, and we identified a gene co-expression module related to meiosis. By alternative splicing analysis, we found that cassette-type exon and alternative start sites were the main forms of alternative splicing in developing gonads. A considerable portion of differentially expressed and alternatively spliced genes were involved in meiosis. CONCLUSION: Taken together, our findings have enriched the gonadal transcriptome database and provided novel candidate genes and avenues to research the molecular mechanisms of sexual differentiation, meiosis, and gametogenesis. SUPPLEMENTARY INFORMATION: Supplementary information accompanies this paper at 10.1186/s12575-019-0108-y. BioMed Central 2019-10-15 /pmc/articles/PMC6794783/ /pubmed/31636514 http://dx.doi.org/10.1186/s12575-019-0108-y Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Wang, Jian
Tian, Geng G.
Zheng, Zhuxia
Li, Bo
Xing, Qinghe
Wu, Ji
Comprehensive Transcriptomic Analysis of Mouse Gonadal Development Involving Sexual Differentiation, Meiosis and Gametogenesis
title Comprehensive Transcriptomic Analysis of Mouse Gonadal Development Involving Sexual Differentiation, Meiosis and Gametogenesis
title_full Comprehensive Transcriptomic Analysis of Mouse Gonadal Development Involving Sexual Differentiation, Meiosis and Gametogenesis
title_fullStr Comprehensive Transcriptomic Analysis of Mouse Gonadal Development Involving Sexual Differentiation, Meiosis and Gametogenesis
title_full_unstemmed Comprehensive Transcriptomic Analysis of Mouse Gonadal Development Involving Sexual Differentiation, Meiosis and Gametogenesis
title_short Comprehensive Transcriptomic Analysis of Mouse Gonadal Development Involving Sexual Differentiation, Meiosis and Gametogenesis
title_sort comprehensive transcriptomic analysis of mouse gonadal development involving sexual differentiation, meiosis and gametogenesis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6794783/
https://www.ncbi.nlm.nih.gov/pubmed/31636514
http://dx.doi.org/10.1186/s12575-019-0108-y
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