Cargando…
Heterologous overexpression of bacterial hemoglobin VHb improves erythritol biosynthesis by yeast Yarrowia lipolytica
BACKGROUND: Yarrowia lipolytica is an unconventional yeast with a huge industrial potential. Despite many advantages for biotechnological applications, it possesses enormous demand for oxygen, which is a bottleneck in large scale production. In this study a codon optimized bacterial hemoglobin from...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6794898/ https://www.ncbi.nlm.nih.gov/pubmed/31615519 http://dx.doi.org/10.1186/s12934-019-1231-9 |
_version_ | 1783459390003609600 |
---|---|
author | Mirończuk, Aleksandra M. Kosiorowska, Katarzyna E. Biegalska, Anna Rakicka-Pustułka, Magdalena Szczepańczyk, Mateusz Dobrowolski, Adam |
author_facet | Mirończuk, Aleksandra M. Kosiorowska, Katarzyna E. Biegalska, Anna Rakicka-Pustułka, Magdalena Szczepańczyk, Mateusz Dobrowolski, Adam |
author_sort | Mirończuk, Aleksandra M. |
collection | PubMed |
description | BACKGROUND: Yarrowia lipolytica is an unconventional yeast with a huge industrial potential. Despite many advantages for biotechnological applications, it possesses enormous demand for oxygen, which is a bottleneck in large scale production. In this study a codon optimized bacterial hemoglobin from Vitreoscilla stercoraria (VHb) was overexpressed in Y. lipolytica for efficient growth and erythritol synthesis from glycerol in low-oxygen conditions. Erythritol is a natural sweetener produced by Y. lipolytica under high osmotic pressure and at low pH, and this process requires high oxygen demand. RESULTS: Under these conditions the VHb overexpressing strain showed mostly yeast-type cells resulting in 83% higher erythritol titer in shake-flask experiments. During a bioreactor study the engineered strain showed higher erythritol productivity (Q(ERY) = 0.38 g/l h) and yield (Y(ERY) = 0.37 g/g) in comparison to the control strain (Q(ERY) = 0.30 g/l h, Y(ERY) = 0.29 g/g). Moreover, low stirring during the fermentation process resulted in modest foam formation. CONCLUSIONS: This study showed that overexpression of VHb in Y. lipolytica allows for dynamic growth and efficient production of a value-added product from a low-value substrate. |
format | Online Article Text |
id | pubmed-6794898 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-67948982019-10-21 Heterologous overexpression of bacterial hemoglobin VHb improves erythritol biosynthesis by yeast Yarrowia lipolytica Mirończuk, Aleksandra M. Kosiorowska, Katarzyna E. Biegalska, Anna Rakicka-Pustułka, Magdalena Szczepańczyk, Mateusz Dobrowolski, Adam Microb Cell Fact Research BACKGROUND: Yarrowia lipolytica is an unconventional yeast with a huge industrial potential. Despite many advantages for biotechnological applications, it possesses enormous demand for oxygen, which is a bottleneck in large scale production. In this study a codon optimized bacterial hemoglobin from Vitreoscilla stercoraria (VHb) was overexpressed in Y. lipolytica for efficient growth and erythritol synthesis from glycerol in low-oxygen conditions. Erythritol is a natural sweetener produced by Y. lipolytica under high osmotic pressure and at low pH, and this process requires high oxygen demand. RESULTS: Under these conditions the VHb overexpressing strain showed mostly yeast-type cells resulting in 83% higher erythritol titer in shake-flask experiments. During a bioreactor study the engineered strain showed higher erythritol productivity (Q(ERY) = 0.38 g/l h) and yield (Y(ERY) = 0.37 g/g) in comparison to the control strain (Q(ERY) = 0.30 g/l h, Y(ERY) = 0.29 g/g). Moreover, low stirring during the fermentation process resulted in modest foam formation. CONCLUSIONS: This study showed that overexpression of VHb in Y. lipolytica allows for dynamic growth and efficient production of a value-added product from a low-value substrate. BioMed Central 2019-10-15 /pmc/articles/PMC6794898/ /pubmed/31615519 http://dx.doi.org/10.1186/s12934-019-1231-9 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Mirończuk, Aleksandra M. Kosiorowska, Katarzyna E. Biegalska, Anna Rakicka-Pustułka, Magdalena Szczepańczyk, Mateusz Dobrowolski, Adam Heterologous overexpression of bacterial hemoglobin VHb improves erythritol biosynthesis by yeast Yarrowia lipolytica |
title | Heterologous overexpression of bacterial hemoglobin VHb improves erythritol biosynthesis by yeast Yarrowia lipolytica |
title_full | Heterologous overexpression of bacterial hemoglobin VHb improves erythritol biosynthesis by yeast Yarrowia lipolytica |
title_fullStr | Heterologous overexpression of bacterial hemoglobin VHb improves erythritol biosynthesis by yeast Yarrowia lipolytica |
title_full_unstemmed | Heterologous overexpression of bacterial hemoglobin VHb improves erythritol biosynthesis by yeast Yarrowia lipolytica |
title_short | Heterologous overexpression of bacterial hemoglobin VHb improves erythritol biosynthesis by yeast Yarrowia lipolytica |
title_sort | heterologous overexpression of bacterial hemoglobin vhb improves erythritol biosynthesis by yeast yarrowia lipolytica |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6794898/ https://www.ncbi.nlm.nih.gov/pubmed/31615519 http://dx.doi.org/10.1186/s12934-019-1231-9 |
work_keys_str_mv | AT mironczukaleksandram heterologousoverexpressionofbacterialhemoglobinvhbimproveserythritolbiosynthesisbyyeastyarrowialipolytica AT kosiorowskakatarzynae heterologousoverexpressionofbacterialhemoglobinvhbimproveserythritolbiosynthesisbyyeastyarrowialipolytica AT biegalskaanna heterologousoverexpressionofbacterialhemoglobinvhbimproveserythritolbiosynthesisbyyeastyarrowialipolytica AT rakickapustułkamagdalena heterologousoverexpressionofbacterialhemoglobinvhbimproveserythritolbiosynthesisbyyeastyarrowialipolytica AT szczepanczykmateusz heterologousoverexpressionofbacterialhemoglobinvhbimproveserythritolbiosynthesisbyyeastyarrowialipolytica AT dobrowolskiadam heterologousoverexpressionofbacterialhemoglobinvhbimproveserythritolbiosynthesisbyyeastyarrowialipolytica |